Expression of pigeonpea hybrid‐proline‐rich protein encoding gene (<i>CcHyPRP</i>) in yeast and <i>Arabidopsis</i> affords multiple abiotic stress tolerance

Priyanka, Bhyri; Sekhar, Kondapalli Venkata Gowri Chandra; Reddy, V. D.; Rao, K. V.

doi:10.1111/j.1467-7652.2009.00467.x

Cited by 86 publications

(73 citation statements)

References 60 publications

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“…However, a gap still exists between the results in the laboratory and the application of these techniques to the staple crops in the field. One of the problems is that the constitutive overexpression of stress-related genes often causes abnormal development and thus a loss in productivity (Kasuga et al, 1999;Priyanka et al, 2010;Dubouzet et al, 2003;Nakashima et al, 2007;Hsieh et al, 2002). The improvement of drought tolerance should be achieved without a parallel limitation of plant growth and yield potential (Cattivelli et al, 2008).…”

Section: Grain Yield Increase Of Atedt1/hdg11 Transgenic Rice With Enmentioning

confidence: 99%

Arabidopsis Enhanced Drought Tolerance1/HOMEODOMAIN GLABROUS11 Confers Drought Tolerance in Transgenic Rice without Yield Penalty

et al. 2013

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Enhancing drought tolerance without yield decrease has been a great challenge in crop improvement. Here, we report the Arabidopsis (Arabidopsis thaliana) homodomain-leucine zipper transcription factor Enhanced Drought Tolerance/HOMEODOMAIN GLABROUS11 (EDT1/HDG11) was able to confer drought tolerance and increase grain yield in transgenic rice (Oryza sativa) plants. The improved drought tolerance was associated with a more extensive root system, reduced stomatal density, and higher water use efficiency. The transgenic rice plants also had higher levels of abscisic acid, proline, soluble sugar, and reactive oxygen species-scavenging enzyme activities during stress treatments. The increased grain yield of the transgenic rice was contributed by improved seed setting, larger panicle, and more tillers as well as increased photosynthetic capacity. Digital gene expression analysis indicated that AtEDT1/HDG11 had a significant influence on gene expression profile in rice, which was consistent with the observed phenotypes of transgenic rice plants. Our study shows that AtEDT1/HDG11 can improve both stress tolerance and grain yield in rice, demonstrating the efficacy of AtEDT1/HDG11 in crop improvement.

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Section: Grain Yield Increase Of Atedt1/hdg11 Transgenic Rice With Enmentioning

confidence: 99%

Arabidopsis Enhanced Drought Tolerance1/HOMEODOMAIN GLABROUS11 Confers Drought Tolerance in Transgenic Rice without Yield Penalty

et al. 2013

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“…For instance, constitutive overexpression of Arabidopsis thaliana DREB1A or rice DREB1A confers freezing and dehydration tolerance to Arabidopsis, but results in severe growth retardation (Kasuga et al, 1999;Liu et al, 1998;Dubouzet et al, 2003). The constitutive expression of a pigeonpea proline-rich protein in Arabidopsis confers tolerance to osmotic, salt, and heat stress, but produces plants that are stunted in size (Priyanka et al, 2010). The cost to plants of defence is therefore likely to be reduced if specific genes and compounds have roles in several different stress responses, a potential explanation for the overlap between stress response pathways (Herms and Mattson, 1992;Bergelson and Purrington, 1996;Asselbergh et al, 2008b).…”

Section: The Importance Of Studying Plant Stresses In Combinationmentioning

confidence: 99%

“…Further transcriptome studies in various species have identified genes, hormones, and processes important in controlling plant response to multiple abiotic or multiple biotic stresses and provided targets for the improvement of stress tolerance (Voelckel and Baldwin, 2004;Hewezi et al, 2008;Priyanka et al, 2010;Wang et al, 2010;Grigorova et al, 2011). A combination of high light and high temperature activated a specific subset of differentially expressed genes in sunflower (Hewezi et al, 2008).…”

Section: Transcriptome Analysis Of Multiple Stress Responsesmentioning

confidence: 99%

“…As plants have finite resources which must be balanced between growth and defence against stresses, often both natural and induced stress tolerance comes with a growth or yield penalty, making it agriculturally disadvantageous (Herms and Mattson, 1992;Baldwin, 1998;Liu et al, 1998;Kasuga et al, 1999;Dubouzet et al, 2003;Tian et al, 2003;Priyanka et al, 2010). Rather than developing crops that can survive extreme stress events, it may therefore be more beneficial to focus on producing crops which are stress tolerant but which maintain high photosynthesis, growth rates, and yield (Condon et al, 2004;Morison et al, 2008;Bechtold et al, 2010;Mullineaux et al, 2011).…”

Section: Perspectives: Seeing Plant Stress Research In a New Lightmentioning

confidence: 99%

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The interaction of plant biotic and abiotic stresses: from genes to the field

Atkinson

Urwin

2012

Journal of Experimental Botany

1,537

976

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In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short-(6 h) and long-(2 d or 4 d) term and low (10 mM) and high (50 mM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants.

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“…Baroowa and Gogoi (2012) also reported that drought induced accumulation of proline in leaves of blackgram and greengram. Priyanka et al (2010) isolated a hybrid-proline-rich protein gene (CcHyPRP) from pigeonpea plants subjected to drought stress and validated its function in Arabidopsis. Srinath et al (2014) reported that abiotic stress tolerance through increased levels of antioxidant enzymes, proline, and reducing sugars in transgenic Arabidopsis with gene (CcCDR) isolated from drought stressed pigeonpea.…”

Section: Effect Of Drought Stress On Proline Contentmentioning

confidence: 99%

Genotypic variability in physiological, biomass and yield response to drought stress in pigeonpea

Vanaja

Maheswari

Sathish

et al. 2015

Physiol Mol Biol Plants

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Three pigeonpea (Cajanus cajan L. Millsp.) genotypes-GT-1, AKP-1 and PRG-158 with varying crop duration, growth habit and flowering pattern were evaluated for variability in their response for drought stress. Drought stress was imposed at initiation of flowering and the observations on biomass and seed yield parameters were recorded at harvest. The magnitude of response of individual component to drought stress was found to be genotype specific. Drought stress significantly decreased photosynthetic rate (P N ), transpiration rate (Tr) and relative water content (RWC) in all the genotypes, however the magnitude of reduction differed with genotype. With drought stress, the reduction of P N was highest in GT-1 while reduction in Tr was highest in PRG-158. The genotype AKP-1, accumulated significantly higher concentrations of osmotic solutes especially proline under water deficit stress, this facilitated it to maintain higher relative water content (RWC) and lower malondialdehyde (MDA) content as compared to other genotypes. Drought stress also impacted biomass production and their partitioning to vegetative and reproductive components at harvest. There was significant variability between the genotypes for seed yield under drought stress while it was non-significant under wellwatered condition. Drought stress enhanced flower drop and decreased flower to pod conversion resulting in reduced pod number and seed number in PRG-158 and GT-1. The genotype AKP-1 recorded superior performance for seed yield under stress environment due to its ability in maintaining pod and seed number as well as improved test weight (100 seed weight). Under drought stress, significant positive association of seed yield with proline, seed number, pod number and test weight clearly indicating their role in drought tolerance.

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Expression of pigeonpea hybrid‐proline‐rich protein encoding gene (CcHyPRP) in yeast and Arabidopsis affords multiple abiotic stress tolerance

Cited by 86 publications

References 60 publications

Arabidopsis Enhanced Drought Tolerance1/HOMEODOMAIN GLABROUS11 Confers Drought Tolerance in Transgenic Rice without Yield Penalty

Arabidopsis Enhanced Drought Tolerance1/HOMEODOMAIN GLABROUS11 Confers Drought Tolerance in Transgenic Rice without Yield Penalty

The interaction of plant biotic and abiotic stresses: from genes to the field

Genotypic variability in physiological, biomass and yield response to drought stress in pigeonpea

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