Expression of phospholipase C β1 in olive flounder (Paralichthys olivaceus) following external stress stimulation

Abstract

In this study, to clarify the function of PoPLC-β1, in response to stress challenge, we examined the PoPLC-β1 expression pattern in response to external stress (pathogen-associated molecular pathogen challenge and environmental challenge including temperature and salinity). PoPLC-β1 expression analysis of tissue from olive flounder showed that the messenger RNA (mRNA) was predominantly expressed in the brain, heart, eye, liver, spleen, and stomach. We also tested the mRNA expression of the PoPLC-β1 in the spleen and kidney of olive flounder by RT-PCR and real-time PCR following stimulation with lipopolysaccharide (LPS), concanavalin A (ConA), or polyinosinic:polycytidylic acid (PolyI:C) and compared with the inflammatory cytokines IL-1b and IL-6 in the stimulated flounder tissues. Each of the spleen and kidney and mRNA transcripts of PoPLC-β1 were increased 30-and 10-fold than normal tissue at 1-6 h post injection (HPI) with PolyI:C when the expression of PoPLC-β1 transcript was similar to LPS and ConA. We also tested the expression of PoPLC-β1 in response to temperature and salinity stress. The expression of PoPLC-β1 also was affected by temperature and salinity stress. Our results provide clear evidence that the olive flounder PLC-β1 signal pathways may play a critical role in immune function at the cellular level and in inflammation reactions. In addition, PLC-β1 appears to act as an oxidative-stress suppressor to prevent cell damage in fish.

Effects of dietary lipid sources on the survival, growth, body composition, antioxidant capacity and expression of antioxidant and pro‐inflammatory genes in juvenile Chinese mitten crab ( Eriocheir sinensis ) reared under three salinities

“…High mortality and low growth rates were usually observed in the areas above possibly due to the salinity stress. It was previously revealed that the physiological state of crustaceans could be altered by high or low salinity stress (Long et al, 2018; Schmitz et al, 2016; Woo et al, 2016). Salinity stress is characterized by an increased inflammatory response and decreased antioxidant capacity in a variety of aquatic animals.…”