Expression of Pancreatic Endocrine Markers by Mesenchymal Stem Cells From Human Umbilical Cord Vein

Santos, Tiago Marcon dos; Percegona, L.S.; Gonzalez, Phillip; Calil, A.; Corradi-Perini, Carla; Faucz, Fábio R.; Câmara, Niels Olsen Saraiva; Aita, Carlos Alberto Mayora

doi:10.1016/j.transproceed.2010.01.032

Cited by 15 publications

(8 citation statements)

References 3 publications

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“…MSCs are multipotent, adherent SCs capable of differentiating into osteoblasts, adipocytes, and chondroblasts [104] and also to nonmesenchymal lineages such as pancreatic islands [105], hepatocytes [106,107], and neuron-like cells [108]. MSCs were first isolated from bone marrow by A.J.…”

Section: Molecular Markers For Msc Characterizationmentioning

confidence: 99%

Reviewing and Updating the Major Molecular Markers for Stem Cells

Calloni

Cordero

Henriques

et al. 2013

Stem Cells and Development

151

116

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Section: Molecular Markers For Msc Characterizationmentioning

confidence: 99%

Reviewing and Updating the Major Molecular Markers for Stem Cells

Calloni

Cordero

Henriques

et al. 2013

Stem Cells and Development

151

116

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“…They are self-renewing cells that can differentiate into a variety of cell types including osteoblasts, chondrocytes, and adipocytes [7], [8] and possibly neuron-like cells [9], [10], hepatocytes [11], or pancreatic-like cells [12]. Due to their multi-lineage differentiation potential and their ability to migrate to injured tissues [13], hMSCs are considered as promising candidates for tissue engineering and regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

The Cell Surface Proteome of Human Mesenchymal Stromal Cells

et al. 2011

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BackgroundMultipotent human mesenchymal stromal cells (hMSCs) are considered as promising biological tools for regenerative medicine. Their antibody-based isolation relies on the identification of reliable cell surface markers.Methodology/Principal FindingsTo obtain a comprehensive view of the cell surface proteome of bone marrow-derived hMSCs, we have developed an analytical pipeline relying on cell surface biotinylation of intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin to enrich the plasma membrane proteins and mass spectrometry for identification with extremely high confidence. Among the 888 proteins identified, we found ≈200 bona fide plasma membrane proteins including 33 cell adhesion molecules and 26 signaling receptors. In total 41 CD markers including 5 novel ones (CD97, CD112, CD239, CD276, and CD316) were identified. The CD markers are distributed homogenously within plastic-adherent hMSC populations and their expression is modulated during the process of adipogenesis or osteogenesis. Moreover, our in silico analysis revealed a significant difference between the cell surface proteome of hMSCs and that of human embryonic stem cells reported previously.Conclusions/SignificanceCollectively, our analytical methods not only provide a basis for further studies of mechanisms maintaining the multipotency of hMSCs within their niches and triggering their differentiation after signaling, but also a toolbox for a refined antibody-based identification of hMSC populations from different tissues and their isolation for therapeutic intervention.

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“…MSCs are multipotent, adherent stem cells capable of differentiating into osteoblasts, adipocytes, and chondroblasts13, as well as endodermal lineages such as pancreatic islands1415 or hepatocytes1617, and ectodermal lineage such as neurons1418. An earlier study verified that human bone marrow-derived MSC (BMCs) are potential progenitors of endometrial stromal cells by demonstrating their ability to decidualize19.…”

mentioning

confidence: 99%

Derivation of human decidua-like cells from amnion and menstrual blood

Sugawara

Hamatani

Yamada

et al. 2014

Sci Rep

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We induced differentiation of human amnion-derived mesenchymal stem cells (AMCs) and menstrual blood-derived mesenchymal stem cells (MMCs) into endometrial stroma-like cells, which could be useful for cell therapy to support embryo implantation. Interestingly, the expression patterns of surface markers were similar among AMCs, MMCs, and endometrial stromal cells. In addition, whereas treatment with estrogen and progesterone was not very effective for decidualizing AMCs and MMCs, treatment with 8-Br-cAMP prompted remarkable morphological changes in these cells as well as increased expression of decidualization markers (prolactin and insulin-like growth factor binding protein-1) and attenuated expression of surface markers unique to mesenchymal stem cells. These results demonstrated that bone marrow-derived stem cells, which are considered a potential source of endometrial progenitor cells, as well as AMCs and MMCs show in vitro decidualization potential, which is characteristic of endometrial stromal cells.

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Expression of Pancreatic Endocrine Markers by Mesenchymal Stem Cells From Human Umbilical Cord Vein

Abstract: The MSC differentiation protocol used in the present study induced expression of some endocrine markers. Insulin was not produced by these cells, probably because of incomplete induction of differentiation.

Cited by 15 publications

References 3 publications

Reviewing and Updating the Major Molecular Markers for Stem Cells

Reviewing and Updating the Major Molecular Markers for Stem Cells

The Cell Surface Proteome of Human Mesenchymal Stromal Cells

Derivation of human decidua-like cells from amnion and menstrual blood

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