Reviewing and Updating the Major Molecular Markers for Stem Cells

Calloni, Raquel; Cordero, Elvira Alicia Aparicio; Henriques, João Antônio Pêgas; Bonatto, Diego

doi:10.1089/scd.2012.0637

Cited by 151 publications

(130 citation statements)

References 231 publications

(251 reference statements)

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“…Our results clearly confirm that stemness markers such as C-KIT, OCT3/4, NANOG, and SOX2 are expressed not only in cultured endometrial epithelial and stromal cells but also in myometrial uterine cells, at the gene and protein level. These transcription factors indicate the pluripotency of the cells and are expressed in ESc, cancer stem cells, and also in cells residing in reproductive organs as well (Calloni et al 2013, Luo et al 2013, Stimpfel et al 2013. NANOG mRNA expression was elevated in stromal uterine cells in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, identification of two other nuclear transcription factors, NANOG and SOX2, allows for determining the presence of stem cells in the uterus. These markers, which are characteristic for pluripotent cells, are expressed in ESc (Calloni et al 2013) and, as recent studies have shown, also in MSc, settled in reproductive organs (Izadpanah et al 2005, Lee et al 2013, Stimpfel et al 2013. Therefore, we selected OCT3/4, NANOG, and SOX2 as the markers of non-differentiated state and pluripotency/multipotency of cells residing in the bovine uterus.…”

Section: Introductionmentioning

confidence: 96%

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Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

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“…Originally identified as a surface marker on myeloid cells (13), CD13 is a widely expressed membrane-bound metalloprotease involved in pleiotropic functions, including enzymatic cleavage of peptides, antigen presentation, and signal transduction that ultimately mediate downstream biological phenomena such as cell adhesion, proliferation, and motility (14). Diverse cell subpopulations (e.g., fibroblasts, pericytes, epithelial cells, tumor-initiating cells, and stem cells) express CD13 (15)(16)(17)(18)(19).…”

mentioning

confidence: 99%

CD13-positive bone marrow-derived myeloid cells promote angiogenesis, tumor growth, and metastasis

Dondossola

Rangel

Guzman-Rojas

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Angiogenesis is fundamental to tumorigenesis and an attractive target for therapeutic intervention against cancer. We have recently demonstrated that CD13 (aminopeptidase N) expressed by nonmalignant host cells of unspecified types regulate tumor blood vessel development. Here, we compare CD13 wild-type and null bone marrow-transplanted tumor-bearing mice to show that host CD13 + bone marrow-derived cells promote cancer progression via their effect on angiogenesis. Furthermore, we have identified CD11b + CD13 + myeloid cells as the immune subpopulation directly regulating tumor blood vessel development. Finally, we show that these cells are specifically localized within the tumor microenvironment and produce proangiogenic soluble factors. Thus, CD11b + CD13 + myeloid cells constitute a population of bone marrow-derived cells that promote tumor progression and metastasis and are potential candidates for the development of targeted antiangiogenic drugs. A ngiogenesis is a rate-limiting step in the development of many solid tumors, making it an attractive target for therapeutic intervention (1). Although pharmacologic modulation of angiogenesis has shown some clinical success, negative factors such as treatment-related plasticity, drug resistance, and tumor diversity have underscored the need for a better understanding of tumor-associated blood vessel formation at a mechanistic level (2). Tumor angiogenesis is a multifactorial process involving several different cell subtypes, especially tumor stromal endothelial cells, pericytes, carcinoma-associated fibroblasts (CAFs), and bone marrow-derived cells (BMDCs) (3, 4). A variety of immune cells directly support angiogenesis, including mast cells, tumor-associated macrophages (TAMs), and Tie2-expressing macrophages (TEMs) (5-7). Endothelial cells recruit inflammatory cells to the extravascular tissue by the expression of different leukocyte adhesion molecules. In turn, immune cells produce soluble factors, such as chemokines, cytokines, and proteases, that influence endothelial cell function and angiogenesis in a paracrine fashion (5). Other studies have shown that BMDCs have the ability to differentiate into endothelial cells, possibly by conversion first to endothelial progenitors (8-10).Proteases activate growth factors and inhibit suppressive factors within tumors and are central to the angiogenic process within the tumor microenvironment (11). Studies on the involvement of aminopeptidases in tumor progression and angiogenesis have revealed a role for aminopeptidase N (CD13) expressed by stromal cells (12). Originally identified as a surface marker on myeloid cells (13), CD13 is a widely expressed membrane-bound metalloprotease involved in pleiotropic functions, including enzymatic cleavage of peptides, antigen presentation, and signal transduction that ultimately mediate downstream biological phenomena such as cell adhesion, proliferation, and motility (14). Diverse cell subpopulations (e.g., fibroblasts, pericytes, epithelial cells, tumor-initiating cells, and st...

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“…[21][22][23] CD44 is a cell surface adhesion molecule that binds to extracellular ligand to regulate hepatic cancer proliferation, differentiation, metastasis and survival. 16,[24][25] Moreover, CD45-positive subpopulations often express other stem cell markers such as CD133 and CD90. 21,26 Yang and co-workers suggest that CD44-positive liver CSC subpopulations are more aggressive than CD44-negative subpopulations.…”

Section: Obtaining Hepatic Cscsmentioning

confidence: 99%

Hierarchical potential differentiation of liver cancer stem cells

Zhang¹,

Feng³

2017

Adv Clin Exp Med

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Hepatocellular carcinoma (HCC) is one of the most malignant tumors in Chinese people and offers poor prognosis. Tumor tissue, like normal tissue, is hierarchically differentiated. Thus, minor tumor cell populations able to differentiate, such as stem cells, sustain tumor self-renewal and proliferation. The fact that liver cancer stem cells (CSCs) with different surface markers appear heterogeneous with respect to oncogenesis and drug resistance indicates that subpopulations of surface markers preserve the hierarchical potential of differentiation during proliferation, deterioration and relapse. The epithelial to mesenchymal transition (EMT) is correlated to tumor malignancy and aggression, and hepatocytes bearing EMT have obvious hierarchical differentiation potential with respect to signaling pathways such as transforming growth factor β, Wnt/β-catenin and microRNA. Therefore, it may be more effective for early diagnosis to monitor HCC recurrence using peripherally circulating CSCs, and these may also offer potential for HCC immunotherapy or for targeting HCC treatment using these markers. Thus, we reviewed the generation, hierarchical differentiation and clinical application of hepatic CSCs.

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Reviewing and Updating the Major Molecular Markers for Stem Cells

Cited by 151 publications

References 231 publications

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

CD13-positive bone marrow-derived myeloid cells promote angiogenesis, tumor growth, and metastasis

Hierarchical potential differentiation of liver cancer stem cells

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