Expression of p53, bcl-2 and growth hormone receptor in atrophic type of actinic keratosis

Stanimirović, Andrija; Čupić, Hrvoje; Bošnjak, Berislav; Tomas, Davor; Baličević, Drinko; Krušlin, Božo; Belicza, Mladen

doi:10.1016/j.jdermsci.2003.11.006

Cited by 5 publications

(14 citation statements)

References 10 publications

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“…We did not determine whether the immunopositivity was related to mutated TP53 or an accumulation of the wild‐type protein, but both possibilities are feasible and may even coexist. The positivity of TP53 protein in both types of analysed AK was located in nucleus and diffusely dispersed through lesions, which is consistent with literature data 34–39 …”

Section: Discussionsupporting

confidence: 92%

“…In the largest series, Shimitzu reported immunohistochemical positivity of TP53 in 58 (85%) from 68 specimens 39 . Our results of prominent TP53 immunopositivity in AK are consistent with reports in the literature 34–39 . In our study, statistical analysis found no significant differences between the expressions of TP53 protein in two investigated AK types.…”

Section: Discussionsupporting

confidence: 92%

“…Feinmesser analysed Bcl‐2 expression in 20 AK and revealed positivity in only 35% of cases 46 . In our study, all investigated AK showed Bcl‐2 expression but the majority was weakly positive, and only 41.7% of investigated AK had stronger immunoreaction than unaffected perilesional skin that is consistent with results in the literature 36,37,46 . Comparison of Bcl‐2 expression in atrophic and hypertrophic AK revealed statistically significant stronger expression in atrophic type.…”

Section: Discussionsupporting

confidence: 90%

“…Although there are many investigations of Tp53 expression in AK, there is no general agreement in the literature about the intensity of TP53‐positive staining of AK. The results vary markedly, from 0% up to 100%, but different authors reported positivity in approximately 63% to 100% of analysed AK specimens 34–39 . In the largest series, Shimitzu reported immunohistochemical positivity of TP53 in 58 (85%) from 68 specimens 39 .…”

Section: Discussionmentioning

confidence: 96%

“…The results vary markedly, from 0% up to 100%, but different authors reported positivity in approximately 63% to 100% of analysed AK specimens. [34][35][36][37][38][39] In the largest series, Shimitzu reported immunohistochemical positivity of TP53 in 58 (85%) from 68 specimens. 39 Our results of prominent TP53 immunopositivity in AK are consistent with reports in the literature.…”

Section: Discussionmentioning

confidence: 97%

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Correlation between Bcl‐2 and Bax in atrophic and hypertrophic type of actinic keratosis

Tomas¹,

Krušlin²,

Čupić³

et al. 2005

Acad Dermatol Venereol

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 97%

See 3 more Smart Citations

Correlation between Bcl‐2 and Bax in atrophic and hypertrophic type of actinic keratosis

Tomas¹,

Krušlin²,

Čupić³

et al. 2005

Acad Dermatol Venereol

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Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis

Çayirli¹,

Köse

Demiriz

2013

Arch Dermatol Res

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Actinic keratoses (AKs) is a keratinocytic neoplasm that typically develops on the face of elderly patients. Little is known regarding the clinical, dermatoscopic and immunohistochemical assessments of AK using topical diclofenac therapy. We sought to determine these assessments and evaluate the efficacy of topical diclofenac gel in AK. In this prospective, open-label study, 44 patients with 66 AKs were treated for 12 weeks with topically applied diclofenac (3% gel in 2.5% hyaluronic acid). Immunohistopathologic analyses were performed before and after diclofenac treatment using epidermal stem cell markers such as Cytokeratin 15 (CK15), Cytokeratin 19 (CK19) and p63, in addition to proliferation markers (Bcl-2, Ki-67). Diclofenac gel was found to be effective in AK, including the hyperkeratotic type. Surprisingly, complete remission was observed at a significantly higher rate in Grade 3 lesions (p = 0.017). However, imunohistochemical and histopathologic examinations revealed that 12-week treatment periods may not be sufficient to fully cure AK. The immunohistochemical analyses revealed no change in the expression levels of CK15, CK19 and Bcl-2 following diclofenac therapy. However, the expression of Ki-67 (p = 0.042) and p63 (p = 0.030) exhibited a significant decrease after therapy. Dermatoscopy is an effective method for diagnosis of AK, and topical diclofenac sodium gel was found as an effective additional treatment modality. Since positive histopathological findings were detected in some patients even with significant remission, a 12-week treatment period should be extended even in patients presenting with positive clinical response. Importantly, anti-proliferative effects of diclofenac were demonstrated by decreased Ki-67 and p63 expression levels.

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Clinical, Histologic, and Immunohistochemical Analyses of Feline Squamous Cell Carcinoma In Situ

et al. 2009

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Actinic keratosis (AK) and Bowenoid in situ carcinoma (BISC) are two distinct forms of in situ squamous cell carcinoma in felines. They usually occur on different locations and present with specific clinical and histologic features. However, in some cases, these diseases cannot be distinguished either clinically or histopathologically. The aim of the present study was to determine the accuracy of diagnosis based on clinical or histologic criteria alone, and whether immunohistochemistry for papillomavirus or p53 can improve the accuracy of diagnosis. A series of in situ squamous cell carcinoma cases (n = 45) were selected according to their location and initial histologic classification and subsequently classified as AK (n = 22) or BISC (n = 23) according to the clinical criteria and were reevaluated histologically by 2 dermatopathologists. All BISC cases and most of the AK cases (n = 15) were confirmed histologically. In 7 cases clinically classified as AK, this diagnosis was not unanimously confirmed histologically because of the presence of overlapping features. P53 immunoreactivity was observed in 11/14 (79%) confirmed AK cases and in 4/22 (18%) BISC cases, while papillomavirus antigen was not detected in any confirmed AK case but was detected in 11/23 (48%) BISC cases. It was concluded that BISC can usually be reliably diagnosed histologically. The histologic diagnosis of lesions clinically suggestive of AK might sometimes be difficult. Results of immunohistochemistry for p53 and papillomavirus antigen were supportive for a role of sun exposure and papillomavirus in the pathogenesis of AK and BISC, respectively.

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Expression of p53, bcl-2 and growth hormone receptor in atrophic type of actinic keratosis

Cited by 5 publications

References 10 publications

Correlation between Bcl‐2 and Bax in atrophic and hypertrophic type of actinic keratosis

Correlation between Bcl‐2 and Bax in atrophic and hypertrophic type of actinic keratosis

Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis

Clinical, Histologic, and Immunohistochemical Analyses of Feline Squamous Cell Carcinoma In Situ

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