Expression of p21 WAF1/CIP1 in Bladder Cancer: Relation to Schistosomiasis

Abstract

SummaryCell cycle regulation is mediated in part through expression of the cyclin-dependent kinase inhibitor p21 WAF1/C IP1 . Loss of p21 WAF1/C IP1 expression may, therefore, contribute partially to schistosomal carcinogenesis in the urinary bladder. We compared p21 WAF1/C IP1 expression in schistosomal and nonschistosomal bladder cancer to explore possible differences in p21 WAF1/C IP1 expression between the two subtypes and the possible association between schistosomiasis and loss of p21 WAF1/C IP1 expression. Tumor specimens were obtained from 130 patients who underwent transurethral biopsy or cystectomy. p21 WAF1 /CIP1 was determined by immunodot blot, Western blot, and enzyme immunoassay (EIA). We validated a highly sensitive quantitative EIA assay for determination of p21 WAF1/C IP1 in cell lysates. Precision, analytical recovery, and linearity were all excellent. Our results did not show any correlation between p21 WAF1 /CIP1 expression and most clinicopathologic variables. Lower expression of p21 WAF1 /CIP1 was evident in squamous cell carcinoma (SCC) and schistosomal subtype than in transitional cell carcinoma and nonschistosomal tumors. Our data suggest a potential role for p21 WAF1/C IP1 alteration in schistosomal carcinogenesis.IUBMB Life, 48: 115± 119, 1999

Expression of cell cycle–related molecular markers in patients treated with radical cystectomy for squamous cell carcinoma of the bladder☆

“…p53 was expressed in 51.3% of SCC cases in our study. Lower expression of p21 in SCC (than UCB) was reported previously [19]. Despite the association between p21 expression and grade of SCC, we could not find an association between the oncological outcome and p21 alteration.…”

“…p53 remains the most frequently studied marker among these markers even in studies addressing SCC of the urinary bladder [7,8,15,[17][18][19][20]22,23]. In this study, p53 was an independent predictor of oncological outcome after RC for SCC.…”

“…Despite the association between p21 expression and grade of SCC, we could not find an association between the oncological outcome and p21 alteration. Eissa et al [19] previously reported that p21 may be independently altered in SCC and might be associated with unfavorable prognosis. In the present study, p27 was the most frequently altered marker among the panel studied, with 97.4% of patients showing p27 alterations.…”

“…However, their prognostic role in SCC of the bladder remains undetermined [7,8,15,[17][18][19][20]22,23]. Previous studies had conflicting reports about the association of these markers with conventional pathologic parameters as well as clinical outcome.…”

Is there a correlation between HPV and urinary bladder carcinoma?

“…In our results, schistosomal-associated tumor showed higher p21 expression compared to non-schistosomal-associated tumor contrary to results of Eissa et al [51].…”