Expression of oxidative phosphorylation genes in renal tumors and tumoral cell lines

Faure, Hélène; Heddi, Abdelaziz; Giraud, Stéphane; Chautard, Denis; Stepien, Georges

doi:10.1002/(sici)1098-2744(199607)16:3<165::aid-mc7>3.0.co;2-g

Cited by 62 publications

(21 citation statements)

References 34 publications

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“…Mostly investigated in the thyroid gland, oncocytic changes are driven by several molecular defects in mitochondrial DNA, and alterations in mitochondrial metabolism have been detected in oncocytic cells and related tumours, including over-expression of regulatory proteins [13], mutations and/or deletions of mitochondrial DNA and Table 1). Histological aspect of the oncocytic tumour which is composed of monomorphic cells with mild nuclear atypia and abundant eosinophilic cytoplasm (a).…”

Section: Discussionmentioning

confidence: 99%

Clinico-pathological features of a series of 11 oncocytic endocrine tumours of the pancreas

et al. 2006

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Oncocytic changes may occur very infrequently in neuroendocrine tumours. To estimate the prevalence, pathological features and clinical behaviour of oncocytic endocrine tumours of the pancreas, we reviewed a series of 227 lesions from two institutions. Eleven cases with predominant oncocytic features were selected, representing 4.85% of the whole series. The morphological features and immunophenotype of such tumours did not differ from conventional endocrine pancreatic tumours, except for the presence of abundant eosinophilic and granular cytoplasm. Anti-mitochondrial antigen was positive in all cases tested, and by electron microscopy, numerous mitochondria were observed in the cytoplasm of tumour cells. The majority of cases were nonfunctioning, and in most cases, pathologic signs of malignancy, leading to a diagnosis of endocrine carcinoma, were observed. In addition, the three nonmalignant cases matched the criteria of well-differentiated tumours of uncertain malignant potential. Nearly 50% of the cases were clinically aggressive, and lymph node and liver metastases were present at the time of diagnosis in a minority of cases. Therefore, oncocytic endocrine pancreatic tumours represent a peculiar morphological and clinical variant characterised by frequent hormonal inactivity and malignant behaviour, which should be considered in the differential diagnosis, especially when dealing with a metastatic lesion.

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Section: Discussionmentioning

confidence: 99%

Clinico-pathological features of a series of 11 oncocytic endocrine tumours of the pancreas

et al. 2006

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“…Decrease in COX activity was found also when comparing the specific activity of the enzyme in biopsies of human colonic adenocarcinoma versus normal colon mucosa [25], in cultured rat HC252 hepatoma cells versus nonneoplastic liver [26], and in cultured carcinoma cell lines MCF-7 (breast), T47D (breast) and DU-145 (prostate) versus normal epithelial cells [27]. FaureVigny et al [28] detected the lower expression of several mitochondrial genes encoding the proteins involved in energy metabolism of cancer cells in renal tumors of different types: a clear cell carcinoma, an oncocytoma, and urothelial tumors. The mean level of expression of COX subunit III gene was found to be lower in carcinoma versus normal mucosa samples [29].…”

Section: Discussionmentioning

confidence: 99%

“…Differential hybridization of one such plasmid DNA blot is shown in Fig. 1 where is possible to see that some cDNA inserts (lanes 10, 26) hybridized more intensive with a GB cDNA probe while the majority (lanes 1,5,9,15,16,19,24,25,28) hybridized more intensive with the normal adult human brain cDNA probe.…”

Section: Identification Of Genes With Altered Expression In Gbs By DImentioning

confidence: 99%

Reduction of the transcription level of the mitochondrial genome in human glioblastoma

et al. 2005

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Screening of human fetal brain cDNA library by glioblastoma (GB) and normal human brain total cDNA probes revealed 80 differentially hybridized clones. Hybridization of the DNA from selected clones and the same cDNA probes confirmed this difference for 38 clones, of which eight clones contained Alu-repeat inserts with increased levels in GB. Thirty clones contained cDNAs corresponding to mitochondrial genes for ATP synthase subunit 6 (ATP6), cytochrome c oxidase subunit II (COXII), cytochrome c oxidase subunit III (COXIII), NADH dehydrogenase subunit 1 (ND1), NADH dehydrogenase subunit 4 (ND4), and mitochondrial 12S rRNA. The levels of all these mitochondrial transcripts were decreased in glioblastomas as compared to tumor-adjacent histologically normal brain. Earlier we found the same for cytochrome c oxidase subunit I (COXI) Serial Analysis of Gene Expression (SAGE) showed lower content of the tags for all mitochondrial genes in GB SAGE libraries and together with our experimental data could serve as evidence of general inactivation of the mitochondrial genome in glioblastoma-the most malignant and abundant form of human brain tumor. q 2004 Published by Elsevier Ireland Ltd.

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“…A peculiar loss of genetic material in 10q in oxyphilic thyroid tumors and renal oncocytomas (Tallini et al 1994), as well as monosomy of chromosome 2 in a subset of Hurthle cell tumors (Tallini et al 1999), has been described. More interestingly, several molecular defects in mitochondrial DNA or alterations in mitochondrial metabolism have been detected in oxyphilic cells and related tumors, including overexpression of regulatory proteins (Faure-Vigny et al 1996), mutations and/or deletions of mitochondrial DNA, and cytochrome C oxidase deficiency (Muller-Hocker 1992;Maximo et al 1998;Muller-Hocker et al 1998). …”

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confidence: 99%

Extensive DNA Fragmentation in Oxyphilic Cell Lesions of the Thyroid

Volante

Papott

Gugliotta

et al. 2001

J Histochem Cytochem.

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S U M M A R YThe in situ end-labeling (ISEL) method demonstrates DNA fragmentation, commonly regarded as a marker of apoptosis. We investigated by the ISEL procedure a series of 52 thyroid lesions, including 24 lesions of mitochondrion-rich oxyphilic cells, both benign and malignant, and 28 non-oxyphilic control tumors. A high percentage of nuclear ISEL staining (approximating to 100% in most cases) was observed in the vast majority of oxyphilic cells from both adenomas and carcinomas, in the absence of morphological apoptotic changes and with no immunocytochemical evidence of caspase activation. This pattern of DNA fragmentation was not observed in non-oxyphilic lesions and was confirmed in total extracted DNA. Moreover, a peculiar cytoplasmic staining was also observed in oxyphilic cells from both benign and malignant lesions, probably related to abnormal fragmentation of mitochondrial DNA. Similar staining patterns were detected in oxyphilic cell tumors of other organs (parathyroids, salivary glands, and kidneys). These findings are consistent with an extensive DNA fragmentation peculiar to oxyphilic cells, which is not directly related to apoptosis and whose origin and biological significance are presently unknown.

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Expression of oxidative phosphorylation genes in renal tumors and tumoral cell lines

Cited by 62 publications

References 34 publications

Clinico-pathological features of a series of 11 oncocytic endocrine tumours of the pancreas

Clinico-pathological features of a series of 11 oncocytic endocrine tumours of the pancreas

Reduction of the transcription level of the mitochondrial genome in human glioblastoma

Extensive DNA Fragmentation in Oxyphilic Cell Lesions of the Thyroid

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