Expression of neutrophil surface markers in icteric neonates before and after phototherapy

Abstract

Background Jaundice due to indirect hyperbilirubinemia affects more than 60% of neonates and phototherapy is the treatment for severe types. There are no previous studies evaluating the effect of phototherapy on the function of neonates neutrophils. The aim of this study was to assess and compare the function of neutrophils by measuring the expression of neutrophils main surface markers in icteric neonates before and after phototherapy. Methods Neonates at a gestational age ≥35 weeks and birth weight ≥2,000 g who met the American Academy of Pediatrics criteria for phototherapy were included. Flow cytometry evaluation of the mean fluorescence intensities of CD10, CD11b, CD11c, CD15, CD16, CD18, CD62L, CD64, and CD66acde was performed before and 24 h after the initiation of phototherapy. Results Twenty‐five neonates at a mean age of 53 h of life were included in the study with a mean bilirubin level of 13.60 ± 2.85 mg/dL. There was no statistical difference in the expression of CD11b, CD15, CD18, CD62L, and CD64 or in the percentage of neutrophils before and after 24 h of phototherapy. There was an increase in the expression of CD10 and CD16 and a decrease in the expression of CD11c and CD66acde after 24 h of phototherapy. Conclusions Newborns submitted to phototherapy had an increase in the expression of CD10 and CD16 and a decreased in the expression of CD11c and CD66acde after 24 h of treatment, which may be related to an anti‐inflammatory effect of phototherapy. © 2018 International Clinical Cytometry Society

Issue Highlights – November 2018

“…Phototherapy is a frequently utilized method in case of icteric newborns and this therapy is usually regarded as a harmless procedure that reduces bilirubin levels and decreases the need for exchange transfusion. In the manuscript of Faulhaber et al it is described that there are several changes in the surface expression of leukocyte markers during phototherapy of newborns. The reason for such studies is substantiated by previous findings since several authors observed side effects associated with phototherapy .…”

Pelaksanaan Terapi Pijat Bayi pada Nenonatus dengan Hiperbilirubin

“…. Fototerapi merupakan penatalaksanaan utama pada kasus peningkatan bilirubin pada neonatus mengikuti kriteria yang direkomendasikan oleh American academy of Pediatrcs (Faulhaber 2018).…”