Expression of NCED gene in colored cotton genotypes subjected to water stress

Souza, Alexandre Muniz Siqueira de; Batista, Vandré Guevara Lyra; Pinheiro, Morganna Pollynne Nóbrega; Suassuna, Janivan Fernandes; Lima, Liziane Maria de; Fernandes, Pedro Dantas

doi:10.1590/1807-1929/agriambi.v20n8p692-696

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…Therefore, the genes that participate in the ABA biosynthetic pathway tend to increase their expression (Zhang et al, 2008;Pan et al, 2010), this is a natural plant response. Despite already having been described in the literature what the ERF8 and NCED genes are responsive to water stress in plants (Cheng et al, 2013;Souza et al, 2016), the mechanism by which these bacteria induce the expression of these genes is unknown.…”

Section: Resultsmentioning

confidence: 99%

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Brito¹,

Santos²,

Barbosa³

et al. 2019

Genet. Mol. Res.

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The peanut plant has high plasticity and great adaptability to adverse conditions, including drought. To mitigate the negative effects of drought on legumes, nitrogen-fixing microorganisms have been investigated in some plant species, such as soybeans and beans. We analyzed the role Bradyrhizobium strains in peanut genotypes subjected to water deficit by means of plant growth, physiological and gene expression analysis. The research was conducted under greenhouse conditions with the runner peanut genotypes (IAC Runner 886, 2012-33 and 2012-47) and two Bradyrhizobium strains (ESA 123 and SEMIA 6144). After 20 days of germination, the water supply was completely interrupted and gas exchange analysis were carried out using an infrared gas analyzer, up to the 10 th day of stress. Leaves were collected for the analysis of proline content and the expression of NCED and ERF8 genes. Analyses of plant height (PH), shoot dry mass (SDM), root dry mass (RDM), number of nodules (NN) and nodule weight (NW) also were ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (4): gmr18379 S.L. Brito et al. 2 performed. The peanut shoots inoculated with the Bradyrhizobium strains obtained the best results. The genotypes inoculated with the ESA 123 strain obtained superior responses compared to the nonstressed treatment inoculated with the same bacteria and the stressed control without bacterial inoculation. In the ESA 123 inoculated treatments, the water stressed plants had higher RDM (28.5% higher, on average), NN (two fold higher), and gene expression (approximately six and threefold higher for ERF8 and NCED genes, respectively). The increase in the expression of NCED and ERF8 genes, in the three genotypes inoculated with ESA 123, suggests a key role of this inoculant in the activation of metabolic cascades for plant protection under water deficit.

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Section: Resultsmentioning

confidence: 99%

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Brito¹,

Santos²,

Barbosa³

et al. 2019

Genet. Mol. Res.

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“…The catalysis of 9- cis -neoxanthin to Xanthoxin by NCED is a key step in the ABA metabolic pathway in plastids [52]. Therefore, a decrease in the expression of NCED seriously affects the expression of other genes in the ABA metabolic pathway, which in turn affects ABA accumulation in the cell [53]. Previous studies have shown that ABA is greatly affected by BR when regulating stomatal opening and guard cell physiological states [54].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Analysis of Masson Pine (Pinus massoniana) under High CO2 Stress

Sun

Zou

et al. 2019

Genes

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To explore the molecular mechanism of the response of Masson pine (Pinus massoniana), the main coniferous tree in southern China, to high CO2 stress, transcriptome sequencing was carried out to analyze the genome-wide responses of annual seedlings under different durations (0 h, 6 h, 12 h and 24 h) of high CO2 stress. The results showed that a total of 3080/1908, 3110/2115 and 2684/1483 genes were up-/down-regulated after 6 h, 12 h and 24 h of treatment, respectively, compared with control check group (CK, 0 h). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of these differentially expressed genes (DEGs) were enriched in energy metabolism, carbohydrate synthesis, cell wall precursor synthesis and hormone regulation pathways. For energy metabolism, the expression of most genes involved in photosynthesis (including the light reaction and Calvin cycle) was generally inhibited, while the expression of genes related glycolysis, the tricarboxylic acid (TCA) cycle and PPP pathway was up-regulated. In addition, the increase in the CO2 concentration induced the up-regulation of gene expression in the sucrose synthesis pathway. Among all starch synthesis genes, GBSS (granule-bound starch synthase) had the highest expression level. On the other hand, during the synthesis of hemicellulose and pectin (cell wall precursor substances), the expression levels of GMD (GDP-mannose 4,6-dehydratase), MGP (Mannose-1-phosphate guanylyl transferase) and RHM (Rhamnose biosynthetic enzyme) were the highest, suggesting that the synthesis of the raw materials hemicellulose and pectin in Masson pine under stress were mainly supplied by GDP-Man, GDP-Fuc and UDP-Rha. Finally, stress inhibited gene expression in the ABA (Abscisic Acid) synthesis pathway and induced gene expression in the GA (Gibberellin), SA (Salicylic acid), BR(Brassinolide) and MeJA (Methyl Jasmonate) pathways. Stomatal switches were regulated by hormonal interactions. This experiment elaborated on the response and molecular mechanism of Masson pine to CO2 stress and aided in screening carbon sequestration genes for the corresponding molecular research of Masson pine in the future.

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“…Under WD conditions, an increase in NCED transcript levels leads to ABA biosynthesis and accumulation in plants, improving drought tolerance by triggering of WD-defense mechanism via hormone induction (Huang et al 2018). NCED gene has been identified in many plant species and lines overexpressing it have shown improved drought tolerance in Arabidopsis (Tong et al 2017), cowpea (Iuchi et al 2001), tomato (Thompson et al 2000), tobacco (Qin and Zeevaart 2002;Pedrosa et al 2017), peanut (Wan and Li 2006), rice (Sultana et al 2014), petunia (Estrada-Melo et al 2015, and cotton (Souza et al 2016). Specifically, in soybean plants GM to overexpress NCED gene, higher levels of ABA were identified in the WD-treated group (Molinari 2020).…”

Section: Expression Profile and Gene Ontology Analysis Of Deg In Flower And Podmentioning

confidence: 99%

Flower and pod genes involved in soybean sensitivity to drought

Molinari

Fuganti-Pagliarini

Marcolino-Gomes

et al. 2021

Journal of Plant Interactions

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In soybean, studies on drought-responses are conducted during vegetative season. Information related to genes triggered in response to water-deficit (WD) in flower and pod is lacking. We performed an RNASeq and an agro-physiological characterization at stages R2 and R4 of soybean cultivar BR16, under WD. Physiological results showed a decrease in gas exchange parameters. Agronomical results showed WD impaired yield. Global Gene Ontology analyses indicated that most of the Differential Expressed Genes (DEGs) were down-regulated in flowers but up-regulated in pods. qRT-PCR revealed that WD triggered hormone biosynthesis changes. Mechanisms such as a decrease in glyceollin levels and cell wall instability were observed. This data showed tissuespecific mechanisms correlated with phenotypic responses such as drought escape, early flowering hormone-induced, and loss in grain weight. We presented here a comprehensive expression profile of flowers and pods soybean genes, which could guide researchers in the development of plants more tolerant to drought.

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Expression of NCED gene in colored cotton genotypes subjected to water stress

Cited by 11 publications

References 18 publications

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Transcriptional Analysis of Masson Pine (Pinus massoniana) under High CO2 Stress

Flower and pod genes involved in soybean sensitivity to drought

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