To explore the molecular mechanism of the response of Masson pine (Pinus massoniana), the main coniferous tree in southern China, to high CO2 stress, transcriptome sequencing was carried out to analyze the genome-wide responses of annual seedlings under different durations (0 h, 6 h, 12 h and 24 h) of high CO2 stress. The results showed that a total of 3080/1908, 3110/2115 and 2684/1483 genes were up-/down-regulated after 6 h, 12 h and 24 h of treatment, respectively, compared with control check group (CK, 0 h). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of these differentially expressed genes (DEGs) were enriched in energy metabolism, carbohydrate synthesis, cell wall precursor synthesis and hormone regulation pathways. For energy metabolism, the expression of most genes involved in photosynthesis (including the light reaction and Calvin cycle) was generally inhibited, while the expression of genes related glycolysis, the tricarboxylic acid (TCA) cycle and PPP pathway was up-regulated. In addition, the increase in the CO2 concentration induced the up-regulation of gene expression in the sucrose synthesis pathway. Among all starch synthesis genes, GBSS (granule-bound starch synthase) had the highest expression level. On the other hand, during the synthesis of hemicellulose and pectin (cell wall precursor substances), the expression levels of GMD (GDP-mannose 4,6-dehydratase), MGP (Mannose-1-phosphate guanylyl transferase) and RHM (Rhamnose biosynthetic enzyme) were the highest, suggesting that the synthesis of the raw materials hemicellulose and pectin in Masson pine under stress were mainly supplied by GDP-Man, GDP-Fuc and UDP-Rha. Finally, stress inhibited gene expression in the ABA (Abscisic Acid) synthesis pathway and induced gene expression in the GA (Gibberellin), SA (Salicylic acid), BR(Brassinolide) and MeJA (Methyl Jasmonate) pathways. Stomatal switches were regulated by hormonal interactions. This experiment elaborated on the response and molecular mechanism of Masson pine to CO2 stress and aided in screening carbon sequestration genes for the corresponding molecular research of Masson pine in the future.

To explore the response of nitrogen metabolism in Masson pine (Pinus massoniana) to high CO2 concentrations, needles from one-year-old seedlings were used as materials to detect key enzyme activities, gene expression and different forms of nitrogen metabolites after CO2 stress for different durations (0 h, 6 h, 12 h, 24 h). The results show that elevated CO2 affected the efficiency of nitrogen metabolism in Masson pine needles, inhibiting the expression of key genes involved in nitrogen metabolism, including glutamate synthase (GOGAT), nitrite reductase (NiR), glutamine synthase (GS), nitrate reductase (NR) and glutamate dehydrogenase (GDH), and decreasing the activities of GOGAT, NiR, and GS. The decrease in enzyme activities and gene expression caused a decrease in different forms of nitrogen metabolites, including total nitrogen, ammonium, nitrite and specific amino acids. With prolonged stress, the nitrate content increased first and then decreased. In this study, the response pattern of nitrogen metabolism to CO2 stress in Masson pine needles was described, which may aid future research on nitrogen utilization in Masson pine.

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Traditional forest inventories are based on field surveys of established sample plots, which involve field measurements of individual trees within a sample plot and the selection of proper allometric equations for tree volume calculation. Thus, accurate field measurements and properly selected allometric equations are two crucial factors for providing high-quality tree volumes. One key problem is the difficulty in accurately acquiring tree height data, resulting in high uncertainty in tree volume calculation when the diameter at breast height (DBH) alone is used. This study examined the uncertainty of tree height measurements using different means and the impact of allometric models on tree volume estimation accuracy. Masson pine and eucalyptus plantations in Fujian Province, China, were selected as examples; their tree heights were measured three ways: using an 18-m telescopic pole, UAV Lidar (unmanned aerial vehicle, light detection and ranging) data, and direct measurement of felled trees, with the latest one as a reference. The DBH-based and DBH–height-based allometric equations corresponding to specific tree species were used for the calculations of tree volumes. The results show that (1) tree volumes calculated from the DBH-based models were lower than those from the DBH–height-based models. On average, tree volumes were underestimated by 0.018 m3 and 0.117 m3 for Masson pine and eucalyptus, respectively, while the relative root-mean-squared errors (RMSEr) were 24.04% and 33.90%, respectively, when using the DBH-based model; (2) the tree height extracted from UAV Lidar data was more accurate than that measured using a telescopic pole, because the pole measurement method generally underestimated the tree height, especially when the trees were taller than the length of the pole (18 m in our study); (3) the tree heights measured using different methods greatly impacted the accuracies of tree volumes calculated using the DBH–height model. The telescopic-pole-measured tree heights resulted in a relative error of 9.1–11.8% in tree volume calculations. This research implies that incorporation of UAV Lidar data with DBH field measurements can effectively improve tree volume estimation and could be a new direction for sample plot data collection in the future.

To compare the rhizosphere communities under Masson pine trees with different carbon sequestration abilities, we sampled three families of Masson pine that showed significant differences in their carbon sequestration ability and conducted high-throughput sequencing of the 16S rRNA and ITS in the corresponding soil samples. The diversity of rhizosphere soil microorganisms, the patterns of differences in relative abundance among the different samples and the responses of microorganisms to environmental factors were analyzed. There was no significant difference in the diversity of soil bacteria or fungi among the different samples from Masson pine families with different carbon sequestration abilities, but with the increase in carbon sequestration, the dominant phyla of bacteria and fungi changed. The dominant phylum of soil bacteria changed from Proteobacteria to Acidobacteria, and that of fungi changed from Ascomycota to Basidiomycota. In addition, we investigated the core microbial communities at the genus level among the different samples, and a significant portion of the core genera were involved in carbon metabolism. The microbial communities were greatly influenced by environmental factors such as soil carbon content, soil moisture and altitude. Soil fungi were more sensitive than soil fungi to the rhizosphere activity of Masson pine.