Expression of natural killer (NK) cell‐specific alloantigens on a mouse NK‐like cell line

Smart, Y. C.; Stevenson, KL; Thorne, R; Thomas, WD; Hsu, LH; Burton, R C

doi:10.1038/icb.1989.36

Cited by 4 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…data). This cell line was produced by culturingCBA spleen cells in TCM containing 30% WEHl-3B (a promyelocytic leukaemia cell line of BALB/c mice; provided by Professor Don Metcalf, Walter and Eliza Hall Institute) culture supernatant (crude interleukin 3 [lL-3]) using previously described methods (9,10) and has been named CBA-IL-3. This CBA-IL-3 cell line has been maintained in culture for about 2 years and has been shown to have only in vitro NC activity.…”

Section: Hyhridoma Selection Strategymentioning

confidence: 99%

Production of a monoclonal allo‐antibody to murine natural cytotoxic cells

Smart

Stevenson

Farrelly

et al. 1990

Immunology & Cell Biology

View full text Add to dashboard Cite

Summary A mouse IgGi monoclonal antibody (1C4), which recognizes a cell surface molecule on murine natural cytotoxic{NC) cells was produced. By flowcytometry. IC4preferentially reacted with less than 5% of fresh CBA spleen cells and 20-50% of CBA-interleukin-3 (lL-3) cells, an in vitro derived NC-like cell line. In vitro treatment of spleen cells from a number of inbred mouse strains either with 1C4 alone or 1C4 coupled to dynabeads markedly decreased or abolished NC activity of the cells against ^'Cr-labelled WEHl-164 targets. Splenic NC activity of these same mouse strains was also reduced or abolished by in vivo administration of IC4. The effect was evident within 2 h of treatment and persisted for at least I week. In contrast IC4 had little or no effect on splenic NK activity against "Cr-labeiled YAC-I targets over the same range of experiments in vitro and in vivo. Results of strain surveys for both in vitro and in vivo reduction of splenic NC activity by 1C4 treatment showed that CBA. C57BL/6, BALB/c and NZB mice were positive and CE and DBA/2 mice were negative, indicating that 1C4 recognizes an allo-antigen on mouse NC cells. This allo-antibody has been designated NC-II, and thus IC4 is an anti-NC-11 monoclonal antibody.

show abstract

Section: Hyhridoma Selection Strategymentioning

confidence: 99%

Production of a monoclonal allo‐antibody to murine natural cytotoxic cells

Smart

Stevenson

Farrelly

et al. 1990

Immunology & Cell Biology

View full text Add to dashboard Cite

show abstract

Monoclonal antibody anti‐NC‐2 identifies a second receptor on cells mediating natural cytotoxicity in mice

et al. 1998

View full text Add to dashboard Cite

SUMMARYWe have previously reported the identification by the murine monoclonal antibody (mAb) 1C4 of the first leucocyte receptor which is involved in natural cytotoxicity (NC ) against WEHI-164; the NC-1.1 receptor. We report herein the identification and characterization of a second leucocyte receptor which is involved in NC, NC-2 (MW 50 000), identified by a rat anti-mouse mAb D9 (immunoglobulin G2a; IgG2a). Flow cytometric analysis showed that NC-2 was expressed on<6% of splenic leucocytes of different inbred mouse strains and 96% of the cells of a mast-cell line which has high NC activity. In vitro treatment of splenic leucocytes with the D9 mAb blocked effector cell-WEHI-164 target cell conjugation and NC by # 50% without affecting natural killing (NK ). Western blot analysis of affinity purified NC-2 and NC-1.1 using the D9 and 1C4 mAbs showed specific reactivity of the proteins with D9 and 1C4, respectively. Pretreatment of splenic leucocytes with both mAbs blocked NC 84%, a result which almost doubled that caused by either mAb alone. Flow cytometric screening of 16 different mouse cell lines showed that 19% of the cell lines expressed both receptors, 6% expressed only NC-2, 44% expressed mainly or only NC-1.1 and the remaining cells expressed neither receptor. These data indicate that D9 identifies a xenoantigen, NC-2, which is expressed on cells mediating NC and not NK, and that it is not the previously described NC-1.1 allo-antigen. We conclude that NC-2 is likely to be one of a number of receptor molecules on cells mediating NC against tumour cells.

show abstract

Adenosine A2A receptor activation reduces hepatic ischemia reperfusion injury by inhibiting CD1d-dependent NKT cell activation

Lappas

Day²,

Marshall³

et al. 2006

The Journal of Experimental Medicine

270

243

View full text Add to dashboard Cite

Ischemia reperfusion injury results from tissue damage during ischemia and ongoing inflammation and injury during reperfusion. Liver reperfusion injury is reduced by lymphocyte depletion or activation of adenosine A2A receptors (A2ARs) with the selective agonist 4- {3-[6-amino-9-(5-ethylcarbamoyl-3,4-dihydroxy-tetrahydro-furan-2-yl)-9H-purin-2-yl]- prop-2-ynyl}-cyclohexanecarboxylic acid methyl ester (ATL146e). We show that NKT cells are stimulated to produce interferon (IFN)-γ by 2 h after the initiation of reperfusion, and the use of antibodies to deplete NK1.1-positive cells (NK and NKT) or to block CD1d-mediated glycolipid presentation to NKT cells replicates, but is not additive to, the protection afforded by ATL146e, as assessed by serum alanine aminotransferase elevation, histological necrosis, neutrophil accumulation, and serum IFN-γ elevation. Reduced reperfusion injury observed in RAG-1 knockout (KO) mice is restored to the wild-type (WT) level by adoptive transfer of NKT cells purified from WT or A2AR KO mice but not IFN-γ KO mice. Additionally, animals with transferred A2AR−/− NKT cells are not protected from hepatic reperfusion injury by ATL146e. In vitro, ATL146e potently inhibits both anti-CD3 and α-galactosylceramide–triggered production of IFN-γ by NKT cells. These findings suggest that hepatic reperfusion injury is initiated by the CD1d-dependent activation of NKT cells, and the activation of these cells is inhibited by A2AR activation.

show abstract

Expression of natural killer (NK) cell‐specific alloantigens on a mouse NK‐like cell line

Cited by 4 publications

References 8 publications

Production of a monoclonal allo‐antibody to murine natural cytotoxic cells

Production of a monoclonal allo‐antibody to murine natural cytotoxic cells

Monoclonal antibody anti‐NC‐2 identifies a second receptor on cells mediating natural cytotoxicity in mice

Adenosine A2A receptor activation reduces hepatic ischemia reperfusion injury by inhibiting CD1d-dependent NKT cell activation

Contact Info

Product

Resources

About