Expression of NAD⁺ dependent 15‐hydroxyprostaglandin dehydrogenase and protection of prostaglandins in human hair follicle

Abstract

NAD(+) dependent 15-hydroxyprostaglandin dehydrogenate (15-PGDH) catalyses oxidation of 15(S)-hydroxyl group of prostaglandins and as a result inactivates their physiological potential. Positive effects of prostaglandins or prostaglandin analogues were reported on terminal hair, vellus hair or eyelash growth and a complex prostaglandin network was recently described in human hair follicle. In the present study, we showed that 15-PGDH was expressed in human hair follicle mainly in melanocytes and keratinocytes, which brought us to consider this enzyme as a possible target to sustain local prostaglandin production. Using a recombinant enzymatic strategy, specific 15-PGDH inhibitors were screened. We identified a thiazolidine dione derivative exhibiting efficacy on follicular outer root sheath keratinocytes, since it concomitantly decreased the production of deactivated 13,14 dihydro 15-ketoprostaglandin F(2alpha) and sustained prostaglandin F(2alpha)in vitro production. In the context of recent interest in prostaglandins and prostaglandin analogues as hair regrowth agents, we postulated that the use of selected 15-PGDH inhibitors could reinforce or prolong the effect of these physiological mediators on hair and skin.

Inhibitors of 15-Prostaglandin Dehydrogenase To Potentiate Tissue Repair

“…For example, scientists at L’Oreal described a series of tetrazoles 15 such as 2 that displayed partial enzyme inhibition at 50 μM and aminooxy amides 16 including 3 , which possessed an IC 50 of 6 μM against the purified enzyme (Figure 2). Cho and colleagues have studied rhodanine alkylidenes such as compound 4 .…”

A guide to hair follicle analysis by transmission electron microscopy: technique and practice

“…Our understanding of the molecular biological aspects of hair follicle development, in particular the signalling pathways involved, has advanced considerably in recent years (1–4). The actions of a number of signalling molecules are intimately entwined with the complex structure of the hair follicle, which is a tiny but complex organ consisting of numerous types of cells and tissues.…”

Identification of the Secondary Follicle Cycle of Hexi Cashmere Goat

“…Morphology research of the secondary follicle cycling of Inner Mongolia cashmere goat was described by Li et al (2008). Research on the molecular biological aspects of hair follicle development, especially the signaling pathways was involved, has advanced in recent years (Fuchs and Horsley,2008; Michelet et al,2008; Schneider et al,2008). Identification of hair follicle characteristic and activity of one and two humped camels, Iranian cashmere goats, and Spanish goats was also reported by Ansari‐Renani (2010a,b) and Wuliji et al (2006), respectively.…”