Expression of mRNA and protein of macrophage colony-stimulating factor and its receptor in human follicular luteinized granulosa cells

Involvement of macrophage colony-stimulating factor (M-CSF) in the function of follicular granulosa cells

“…Serum M-CSF concentration gradually increased and reached a peak on the day of oocyte retrieval in the stimulation cycle, and M-CSF and its receptor were expressed in GCs (12), which suggested that follicles might be one of the sources and the targets of M-CSF, and M-CSF might play an important role in the process of follicular maturation and ovulation as an intraovarian mediator. Until now, there has been a lack of evidence showing the effect of M-CSF on the function of GCs and the regulation of the expression of M-CSF in the GCs, which represent the largest cell mass in the follicle.…”

“…Follicles were aspirated 36 hours after administration of hCG, and FF was obtained from oocyte-bearing follicles. The mean number of follicles per patient was 7 (range [3][4][5][6][7][8][9][10][11][12][13][14], and the size of the follicles was 18-23 mm.…”

“…We have previously reported that there was a high concentration of M-CSF in human FF. The time course studies in the human luteinized granulosa cells (GCs) showed a significant increase of M-CSF and mRNA of M-CSF and its receptor, and the localization of extracellular domain of human M-CSF receptor was demonstrated immunocytochemically on the surface of GCs (12). Granulosa cells lining the vesicular ovarian follicle are the largest cell mass in the follicle and play a key role in the development and maturation of oocyte as well as in the regulation of ovarian cycle through production and secretion of various sex hormones and cytokines.…”

Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles

“…G-CSF has been previously shown to be secreted by granulosa cells at ovulation (13,14), then within the endometrium during the luteal phase, and finally during gestation in the placenta, and early serum increases occurred in case of pregnancy (15). More recently, a randomized controlled trial reported that G-CSF administration significantly increased live-birth rates in patients with recurrent miscarriages (16).…”

Clinical use of colony-stimulating factor-1 in ovulation induction for poor responders

“…Colony-stimulating factor-1 is supplied by both GCs and from the circulation. The CSF-1 mRNA can be detected within GCs in both mice (10) and humans (11,12). Through alternative mRNA splicing and differential post-translational proteolytic processing, CSF-1 can either be secreted into the circulation or expressed…”