Expression of MMR system genes is correlated to NF-kB in patients with oral squamous cell carcinoma

Abstract

<jats:sec><jats:title>Aims</jats:title><jats:p>The aim of this study was to evaluate the mismatch repair gene expression and their correlation with <jats:italic>NF-kB</jats:italic> in patients with oral squamous cell carcinoma (SCC).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Total RNA was isolated from 28 biopsy samples. A control group was composed of 20 volunteers. Differential expression of <jats:italic>hMSH2</jats:italic>, <jats:italic>hMSH6</jats:italic> and <jats:italic>NF-kB</jats:italic> genes was accessed by quantitative PCR.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>There is increased expression of all the analysed genes when the patients were smokers and alcoholics. In addition, there is increased expression of <jats:italic>hMSH2</jats:italic> when SCC was removed from the base of the tongue. There was a correlation between <jats:italic>NF-kB</jats:italic> and <jats:italic>hMSH2</jats:italic> and <jats:italic>hMSH6</jats:italic> as well as between repair genes <jats:italic>hMSH2</jats:italic> and <jats:italic>hMSH6</jats:italic> expression levels. There is increased expression of the <jats:italic>hMSH2</jats:italic> gene in patients with SCC, especially in the alcoholics.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>There is a strong indication that <jats:italic>NF-kB</jats:italic> gene was expressed along with the studied repair genes, evidencing the possibility that this system can be activated by the inflammatory pathway.</jats:p></jats:sec>

NLRC5 promotes endometrial carcinoma progression by regulating NF-κB pathway-mediated mismatch repair gene deficiency

“…This finding proves that NLRC5 can induce dMMR by suppressing the activation of NF-κB and promoting the development of EC. The MMR deficiency is often associated with promoter methylation and inflammation, especially the promoter methylation of MLH1 10,11,39 , But further research is needed to determine whether NF-κB affect MMR promoter methylation through inflammatory pathways, thereby affecting tumor dMMR status.…”

“…One the other hand, dMMR seems contribute to oncotherapy by improving the sensitivity of tumor immunotherapy. The dMMR is often associated with promoter methylation and inflammation 10 , study have also found that MMR may be regulated by inflammatory pathways induced by NF-κB activation in oral cancer 11 . However, the regulation of dMMR in EC is yet to be elucidated, and understanding the upstream events controlling MMR is particularly important for formulating comprehensive strategies and guiding clinical cancer treatment.…”