Expression of MMP2, MMP9 and MMP3 in Breast Cancer Brain Metastasis in a Rat Model

Mendes, Odete; Kim, Hun-Taek; Stoica, George

doi:10.1007/s10585-005-8115-6

Cited by 190 publications

(147 citation statements)

References 45 publications

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“…In particular, several analyses show that expression of MMP9 is a prognostic indicator in breast cancer patients (36,38,39). As a type IV collagenase, it has been regarded as a critical enzyme for destruction of the basement membrane, the first barrier for cancer invasion.…”

Section: Discussionmentioning

confidence: 99%

Clinical Implications of the Influence of Ehm2 on the Aggressiveness of Breast Cancer Cells through Regulation of Matrix Metalloproteinase-9 Expression

Ye²,

Mansel³

et al. 2010

Molecular Cancer Research

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Ehm2, a member of NF2/ERM/4.1 superfamily, has been indicated in disease progression and metastasis of prostate cancer. However, its function and implication in malignancies remain largely unknown. The present study aimed to examine the role of Ehm2 in breast cancer. We first constructed a hammerhead ribozyme transgene to knock down Ehm2 expression in breast cancer cells. The effect on growth, cell matrix adhesion, motility, and invasion following knockdown of Ehm2 was then investigated using in vitro models. Reduction of Ehm2 had inhibitory effects on in vitro growth and invasion of breast cancer cells. Flow cytometric analysis showed that knockdown of Ehm2 induced apoptosis. Knockdown of Ehm2 also significantly decreased matrix metalloproteinase 9 mRNA and protein levels, as well as the corresponding enzymatic activity, and consequently led to a reduction of the invasion. The expression pattern of Ehm2 in a cohort of breast specimens (normal, n = 33; cancer, n = 127) was analyzed using both quantitative real-time PCR and immunohistochemical staining. Increased expression of Ehm2 in breast cancer was seen at both mRNA and protein levels. Higher levels of Ehm2 transcripts were correlated with disease progression, metastasis, and poor prognosis. Disease-free survival of the patients with lower levels of Ehm2 was 135.8 (95% confidence interval, 125.1-146.5) months, significantly longer compared with 102.5 (95% confidence interval, 78.7-126.4) months of patients with higher levels of Ehm2 expression (P = 0.039). Taken together, increased Ehm2 expression correlates with poor prognosis and metastasis. Ehm2 may promote the invasive ability of breast cancer cells via regulation of matrix metalloproteinase 9. Mol Cancer Res; 8(11); 1501-12.

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Section: Discussionmentioning

confidence: 99%

Clinical Implications of the Influence of Ehm2 on the Aggressiveness of Breast Cancer Cells through Regulation of Matrix Metalloproteinase-9 Expression

Ye²,

Mansel³

et al. 2010

Molecular Cancer Research

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“…The MMPs, a family of proteolytic enzymes that degrade different components of the extracellular matrix, play important roles in the different stages of tumour progression. Matrix metalloproteinase 3 (stromelysin 1) plays an important role in breast cancer progression, and it has shown to have increased levels, as well as activity, in breast cancer brain metastasis in a rat model (Mendes et al, 2005). Similar to AKT, MMP3 can also cause epithelial -mesenchymal transition in cancer (Radisky et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Molecular profiling of cervical cancer progression

Hagemann

Božanović²,

Hooper

et al. 2007

Br J Cancer

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Most cancer patients die of metastatic or recurrent disease, hence the importance to identify target genes upregulated in these lesions. Although a variety of gene signatures associated with metastasis or poor prognosis have been identified in various cancer types, it remains a critical problem to identify key genes as candidate therapeutic targets in metastatic or recurrent cancer. The aim of our study was to identify genes consistently upregulated in both lymph node micrometastases and recurrent tumours compared to matched primary tumours in human cervical cancer. Taqman Low-Density Arrays were used to analyse matched tumour samples, obtained after laser-capture microdissection of tumour cell islands for the expression of 96 genes known to be involved in tumour progression. Immunohistochemistry was performed for a panel of up-and downregulated genes. In lymph node micrometastases, most genes were downregulated or showed expressions equal to the levels found in primary tumours. In more than 50% of lymph node micrometastases studied, eight genes (AKT, BCL2, CSFR1, EGFR1, FGF1, MMP3, MMP9 and TGF-b) were upregulated at least two-fold. Some of these genes (AKT and MMP3) are key regulators of epithelial -mesenchymal transition in cancer. In recurrent tumours, almost all genes were upregulated when compared to the expression profiles of the matched primary tumours, possibly reflecting their aggressive biological behaviour. The two genes showing a consistent downregulated expression in almost all lymph node metastases and recurrent tumours were BAX and APC. As treatment strategies are very limited for metastatic and recurrent cervical cancer, the upregulated genes identified in this study are potential targets for new molecular treatment strategies in metastatic or recurrent cervical cancer.

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“…Lungs, bone and brain are the most common sites for breast cancer metastasis and the occurrence of distant metastases severely limits the prognosis of breast cancer patients. Factors, such as neoplastic cell molecular, genetic characteristics and biological environment, are thought to be determinant in the metastatic process (Chambers et al, 2002;Mendes et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Momordica cochinchinensis Seed Extracts Suppress Migration and Invasion of Human Breast Cancer ZR-75-30 Cells Via Down-regulating MMP-2 and MMP-9

Zheng¹,

Zhang²,

Zhan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Expression of MMP2, MMP9 and MMP3 in Breast Cancer Brain Metastasis in a Rat Model

Cited by 190 publications

References 45 publications

Clinical Implications of the Influence of Ehm2 on the Aggressiveness of Breast Cancer Cells through Regulation of Matrix Metalloproteinase-9 Expression

Clinical Implications of the Influence of Ehm2 on the Aggressiveness of Breast Cancer Cells through Regulation of Matrix Metalloproteinase-9 Expression

Molecular profiling of cervical cancer progression

Momordica cochinchinensis Seed Extracts Suppress Migration and Invasion of Human Breast Cancer ZR-75-30 Cells Via Down-regulating MMP-2 and MMP-9

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