Expression of MMP-13 in human temporomandibular joint disc derangement and osteoarthritis

Perotto, Juliano Henrique; Camejo, Flavio de Alcântara; Doetzer, Andrea Duarte; Almeida, Luís Eduardo; Azevedo, Marina Luise Viola de; Olandoski, Márcia; Noronha, Lúcia de; Trevilatto, Paula Cristina

doi:10.1080/08869634.2017.1315511

Cited by 10 publications

(14 citation statements)

References 27 publications

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“…Until recently, immunohistochemical assays on human TMJ discs were based on subjective assessments of biomarkers' expression. Computerised histomorphometry of TMJ discs has been applied for the investigation of internal derangement and seldom for TMJOA 12,32,48 . We determined both immunohistochemistry results and collagen degeneration through histomorphometric image analysis in an effort to strengthen the validity of results and allow reproducibility.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

Fernandes

Brancher

Michels

et al. 2020

J of Oral Rehabilitation

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Background Temporomandibular joint osteoarthritis (TMJOA) is a progressive degenerative disease caused by imbalance between anabolic and catabolic stimuli. Objective The aim of this study was to evaluate histopathological changes, collagen degeneration and the expression of eleven TMJOA biomarkers in articular discs. Methods Specimens were obtained from eight female patients submitted to discectomy. Discs were divided into anterior band (AB), intermediate zone (IZ) and posterior band (PB) for computerised histomorphometric analyses. Each was assigned a histopathological degeneration score (HDS). Collagen degeneration was assessed with Picrosirius‐polarisation method. Biomarkers were evaluated through immunohistochemistry, including IGF‐1, OPG, VEGF, TNF‐α, FGF‐23, IHH, MMP‐3, MMP‐9, TGF‐β1, BMP‐2 and WNT‐3. Image processing software was used to calculate average immature collagen ratios and immunostained areas. Spearman rank tests were applied to verify correlations, with significance level of 0.05. Results The HDS showed negative correlation with expression of VEGF in IZ and PB (P < .05) and positive with TNF‐α in AB (P < .01). Collagen degeneration correlated with TGF‐β1 (P < .05), BMP‐2 (P < .01) and IHH (P < .05) immunostained areas in the IZ; TGF‐β1, BMP‐2 and IHH expression correlated among each other in AB and IZ (P < .05). Conclusion Angiogenesis and tissue fragmentation may result from aberrant physiologic responses mediated by VEGF and TNF‐α, compromising TMJ discs during OA progression. The expression of TGF‐β1, BMP‐2 and IHH could be related to collagen degeneration in displaced discs and may participate in TMJOA pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

Fernandes

Brancher

Michels

et al. 2020

J of Oral Rehabilitation

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“…Notably results showed that PSO alone have some inhibitory effects on the expression of MMP-13, which is specific for cartilage degeneration. Some studies demonstrated that MMP-13 over-expression can induce the onset of OA through excessive ECM degradation 24, 25. It is further said that MMP-13 levels correlate with the presence of pathological chondrocytes that undergo hypertrophic differentiation in the early stage of OA development 23.…”

Section: Discussionmentioning

confidence: 99%

Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis

Wang

Al-ani²,

Sha³

et al. 2019

Int. J. Biol. Sci.

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Current study examined whether psoralen (PSO) exhibits anti-inflammatory responses, protection and activation of chondrocytes, and relieve osteoarthritis (OA). Rats chondrocytes and human synoviocytes were cultured in tumor necrosis factor-α (TNF-α) conditioned culture medium with/without PSO to test the cell morphologies and cytotoxicities in vitro. Cartilaginous extracellular matrix (ECM) and proliferative gene/protein expression levels were evaluated in chondrocytes. Meanwhile, matrix metalloproteinases (MMPs) and interleukins (ILs) gene/protein expression were analyzed in synoviocytes. SD rats of monosodium iodoacetate (MIA) induced OA model were used in order to assess the effects of PSO on attenuating degeneration of the articular cartilage in vivo. Results showed TNF-α conditioned culturing with/without PSO (1-100 µM) had no any toxicity on both the cell lines. PSO (10 µM) activated cartilaginous specific ECM expression along with up-regulation of proliferative genes at transcriptional levels. Interestingly, PSO significantly reversed TNF-α induced up-regulation of MMP13 and ILs synoviocytes in a dose-dependent manner (1 to 20 µM), while down-regulated cartilaginous ECM production. Following six weeks of PSO treatments to articular cartilage osteoarthritis, compared to MIA-induced group, the appearance and physiological structure of articular cartilage was more integrated with greatly organized chondrocytes and abundant cartilage matrix. In conclusion, PSO protects and activates chondrocytes, antagonizing the expression of MMPs and ILs secreted by synovial cells, and effectively attenuates MIA-induced OA.

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“…As widely accepted, IL-1β is a key mediator in accelerating the activity of MMPs (Ge et al, 2009). MMP13, a representative of MMPs in OA or OA-like changes (de Rooy et al, 2014; Agere et al, 2017; Li et al, 2017; Perotto et al, 2018), reported to be a factor contributes to the early stage development of OA. TMJ-OA is a common disorder in mandibular joint, however, the molecular networks have not been characterized in detail.…”

Section: Discussionmentioning

confidence: 99%

Potential Novel Prediction of TMJ-OA: MiR-140-5p Regulates Inflammation Through Smad/TGF-β Signaling

Zhao

Yang

et al. 2019

Front. Pharmacol.

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Temporomandibular joint osteoarthritis (TMJ-OA), mainly exhibit extracellular matrix loss and condylar cartilage degradation, is the most common chronic and degenerative maxillofacial osteoarthritis; however, no efficient therapy for TMJ-OA exists due to the poor understanding of its pathological progression. MicroRNA (miR)-140-5p is a novel non-coding microRNAs (miRNAs) that expressed in osteoarthritis specifically. To investigate the molecular mechanisms of miR-140-5p in TMJ-OA, primary mandibular condylar chondrocytes (MCCs) from C57BL/6N mice were treated with interleukins (IL)-1β or transfected with miR-140-5p mimics or inhibitors, respectively. The expression of matrix metallopeptidase (MMP)-13, miR-140-5p, nuclear factor (NF)-kB, Smad3 and transforming growth factor (TGF)-β3 were examined by western blotting or quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The interaction between the potential binding sequence of miR-140-5p and the 3′-untranslated region (3′UTR) of Smad3 mRNA was testified by dual-luciferase assay. Small Interfering RNA of Smad3 (Si-Smad3) was utilized to further identify the role of Smad3 mediated by miR-140-5p. The data showed MMP13, miR-140-5p and NF-kB increased significantly in response to IL-1β inflammatory response in MCCs, meanwhile, Smad3 and TGF-β3 reduced markedly. Moreover, transfection of miR-140-5p mimics significantly suppressed the expression of Smad3 and TGF-β3 in MCCs, while miR-140-5p inhibitors acted in a converse manner. As the luciferase reporter of Smad3 mRNA observed active interaction with miR-140-5p, Smad3 was identified as a direct target of miR-140-5p. Additionally, the expression of TGF-β3 was regulated upon the activation of Smad3. Together, these data suggested that miR-140-5p may play a role in regulating mandibular condylar cartilage homeostasis and potentially serve as a novel prognostic factor of TMJ-OA-like pathology.

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Expression of MMP-13 in human temporomandibular joint disc derangement and osteoarthritis

Abstract: This study suggests MMP-13 is not significantly involved in collagen degradation in human TMJ disc displacement or osteoarthrosis.

Cited by 10 publications

References 27 publications

Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

Psoralen Protects Chondrocytes, Exhibits Anti-Inflammatory Effects on Synoviocytes, and Attenuates Monosodium Iodoacetate-Induced Osteoarthritis

Potential Novel Prediction of TMJ-OA: MiR-140-5p Regulates Inflammation Through Smad/TGF-β Signaling

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