Expression of miR‑204 in pediatric retinoblastoma and its effects on proliferation and apoptosis of cancer cells

Abstract

Expression, clinical significance and molecular mechanism of miR-204 in human retinoblastoma (RB) and para-carcinoma tissues were investigated. A total of 110 cases of RB tissues preserved after ophthalmectomy in the First Affiliated Hospital of Hunan Normal University (People's Hospital of Hunan Province) from April 2013 to June 2017 were collected along with 100 cases of para-carcinoma normal tissues. The expression of miR-204 in RB tissues was detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and its associations with clinicopathological features were analyzed. Y79 cells were transfected with miR-204 mimics. A total of 80 pmol/l miR-204 mimics and 10 µl Lipofectamine 2000 were added into the experimental group. Cell proliferation was detected via methyl thiazolyl tetrazolium (MTT) assay at 24, 48, 72 and 96 h, apoptosis was detected via flow cytometry at 48 h after transfection, and the relative expression levels of B-cell lymphoma 2 (Bcl-2) messenger RNA (mRNA) and Sirt1 mRNA were detected via RT-qPCR. The results of MTT assay revealed that the measured value of the optical density (OD) in the experimental group at 48 h was obviously lower than that in the negative control group (p<0.001). The proportion of apoptotic cells in the experimental group was remarkably higher than that in the negative control group (p<0.001). Compared with those in the negative control group, the relative expression levels of Bcl-2 and Sirt1 mRNAs in the experimental group were significantly decreased (p<0.001). miR-204 may be involved in the occurrence and development of RB, which is significantly associated with clinical tissue differentiation, neural infiltration and lymph node metastasis in patients. miR-204 may inhibit proliferation and promote apoptosis of RB cells through downregulating the expression of Bcl-2 and Sirt1 in RB. Therefore, miR-204 may become a new biological index for early diagnosis, prognosis evaluation and biotherapy of RB.

The clinical diagnostic value of plasma miR-592 and miR-217-3p levels in retinoblastoma

“…For example, the expression of miR-204 in Rb patients is dysregulated, and its expression level is related to optic nerve infiltration, lymph node metastasis, and tumour tissue differentiation. miR-204 may affect the proliferation and apoptosis of Rb cells, thereby affecting the development of Rb, which may become an early stage molecular indicator for diagnosing and evaluating the prognosis of Rb (43)(44)(45); miR-338-5p is up-regulated in the serum of patients with Rb, which may play a role in promoting cancer in the occurrence and development of Rb, and has the potential value of the early diagnosis of Rb (46,47). Recent studies have shown that dysregulated miR-592 and miR-217-3p have been reported to be involved in developing many different types of cancer.…”

Small RNA sequencing of pectoral muscle tissue reveals microRNA‐mediated gene modulation in chicken muscle growth

“…Another group also reported that miR‐211 could modulate cell proliferation through CXCR4 and MAPK signalling (Li, Lv, Wang, & Kuang, ). miR‐204 was reported to inhibit cell proliferation and promote apoptosis through the downregulation of Bcl‐2 and Sirt1 (Ding & Lu, ), and was also associated with adipogenesis (He et al, ). These significant differences in expression suggested that these miRNAs, which promote or impede cell proliferation, might be related to muscle growth and development between SMB chicken and DH chicken.…”

LINC00202 promotes retinoblastoma progression by regulating cell proliferation, apoptosis, and aerobic glycolysis through miR-204-5p/HMGCR axis

“…MiR-204-5p exerted antitumor activity by suppressing melanoma cell invasion [ 29 ]. More importantly, miR-204 was found to be decreased in RB, and miR-204 re-expression inhibited RB cell tumorigenesis and progression [ 2 , 30 ]. In this study, we confirmed miR-204-5p was a target of LINC00202, and miR-204-5p expression was negatively regulated by LINC00202 in RB cells.…”

“…Retinoblastoma (RB) is an aggressive intraocular malignancy of infant and childhood, around accounting for 2.5–4% of all pediatric malignancies [ 1 ]. Early and effective treatment can preserve part of the visual function and achieve a longer survival time in children with RB; however, the optimal treatment time will be delayed if there is no timely diagnosis and treatment, thus resulting in a poor prognosis in children with RB [ 2 , 3 ]. Therefore, pinpointing the fundamental mechanism in the occurrence and development of RB and finding new targets to improve the treatment and prognosis of RB are necessary.…”