Expression of microRNAs, CYP1A1 and CYP2B1 in the livers and ovaries of female rats treated with DDT and PAHs

Чанышев, М. Д.; Kosorotikov, N.I.; Титов, С. Е.; Kolesnikov, N. N.; Gulyaeva, L. F.

doi:10.1016/j.lfs.2014.03.031

Cited by 32 publications

(19 citation statements)

References 21 publications

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“…Recent findings about the expression of mRNA and miRNA in ovarian tissue have provided much evidence which has enhanced our understanding of the molecular mechanism of the complex cellular events which occur in this tissue site (Chanyshev et al . ; Pereira et al . ).…”

Section: Discussionmentioning

confidence: 99%

Application of talcum powder, trichloroacetic acid and silver nitrate in female rats for non‐surgical sterilization: evaluation of the apoptotic pathway mRNA and miRNA genes

Yumrutaş

Kara

Atılgan

et al. 2015

Int J Experimental Path

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There are several methods used for non-surgical sterilization in birth control including quinacrine, trichloroacetic acid (TCA), erythromycin, tetracycline, silver nitrate and talcum powder. Among these, talcum powder, TCA and silver nitrate are the most commonly used. However, the toxic and carcinogenic activities of these chemicals in ovarian tissue have been poorly elucidated. This study demonstrates the expression levels of antioxidant, apoptotic and anti-apoptotic genes after administration of talc powder, TCA and silver nitrate for non-surgical sterilization in female rat models. The expression changes of some microRNAs (miR-15b, miR-21, miR-34a and miR-98) that play key roles in the apoptosis pathway were also included. All expression analyses were evaluated with real-time PCR. The expression levels of all genes appeared to be upregulated in the talcum powder group, but the results were not statistically significant. Increased expression of Gsr and Sod1 genes was statistically significant in the talcum powder group. In TCA and silver nitrate group, expression of all genes was appeared to be elevated but only the Gsr expression was statistically significant in the TCA-administrated group; there were no statistically significant changes in the silver nitrate group. miRNA expression levels were increased in talcum powder and TCA-administrated groups, but these results were not significant. Expression levels of miR-15b, miR-21 and miR-98 in the silver nitrate group were significantly increased. Consequently, these chemicals appear to be non-carcinogenic agents for rat ovarian tissue which do not induce apoptosis. However, talcum powder and TCA can be considered as agents that are toxic to ovarian tissue.

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Section: Discussionmentioning

confidence: 99%

Application of talcum powder, trichloroacetic acid and silver nitrate in female rats for non‐surgical sterilization: evaluation of the apoptotic pathway mRNA and miRNA genes

Yumrutaş

Kara

Atılgan

et al. 2015

Int J Experimental Path

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“…Для контроля специфичности ПЦР получали кривые плавления после добавления красителя SYBR в реакционную смесь по окончании реакции. Относительный уровень экспрессии микроРНК определяли по значению пороговых циклов Сt с учетом эффективности реакции, которую оценивали по увеличению сигнала за цикл на экспоненциальной стадии амплификации исследуемой микроРНК и референсной РНК U 6 . ПЦР проводили в следующих условиях: предварительный прогрев при 95°С -3 мин, затем 50 основных циклов: денатурация при 95°С -15 с, отжиг при 58°С -20 с., элонгация и сбор данных по флуоресценции при 72°С -30 с.…”

Section: пцр в реальном времениunclassified

“…Значения свободной энергии дуплексов miR-143 и miR-152 с мРНК-мишенью составляли 19 Можно предположить, что снижение экспрессии данных микроРНК в печени дает "зелёный свет" синтезу белка, что важно для метаболизма ксенобиотиков, окисляемых CYP1A1. Эти результаты подтвердили полученные нами ранее данные по снижению экспрессии miR-21, -221, -222, и -429 в печени самок крыс, обработанных различными ксенобиотиками [6]. Таким образом, мы не выявили зависимых от пола изменений в профиле экспрессии данных микроРНК при индукции БП и ДДТ.…”

Section: результаты и обсуждениеunclassified

“…Таким образом, мы не выявили зависимых от пола изменений в профиле экспрессии данных микроРНК при индукции БП и ДДТ. Ранее, при проведении биоинформатического анализа мы не разделяли микроРНК, специфичные к мРНК CYP2B/1A [6]. В настоящей работе такой анализ проведен с учётом свободной энергии дуплексов микроРНК-мРНК.…”

Section: результаты и обсуждениеunclassified

“…Экспрессия микроРНК может меняться под действием как генотоксичных [4], так и негенотоксичных соединений, таких как тетрахлордибензодиоксин [5]. Нами показано, что экспрессия некоторых онкогенных микроРНК изменяется в разных органах самок крыс, обработанных индукторами цитохрома Р450 [6]. МикроРНК влияют на активность CYP1B1, окисляющего эстрогены, что может приводить к гормональному дисбалансу [7].…”

Section: Introductionunclassified

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Effect of xenobiotics on microrna expression in rat liver

et al. 2016

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Using bioinformatics analysis we selected microRNAs which could bind 3'-UTR-region of cytochrome P450 (CYP) genes. Three microRNA miR-21, -221, -222, their potential targets might be mRNA for CYP1A1, and two microRNA miR-143, miR-152 for CYP2B1 accordingly were selected for experimental verification. Expression level of these microRNAs in rat liver upon benzo(a)pyrene (BP), phenobarbital (PB), and DDT induction was determined using RT-qPCR method. In rats treated by both BP, and DDT the hepatic content of miR-21, -221, -222 significantly demonstrated a 2-3-fold decrease. The decrease in miR expression was accompanied by a considerable (5.5-8.7-fold) increase in the CYP1A1-mediated EROD activity. The expression of miR-143 remained unchanged after the PB treatment, while the expression of miR-152 increased by 2 times, however, the (10.5-fold) increase in PROD activity of CYP2B was much higher. In the DDT-treated liver PROD activity increased by 20 times, the expression of miR-152 didn't change, and the expression of miR-143 increased by 2 times. The bioinformatics analysis of interactions between microRNAs and targets showed that the studied miRs can potentially bind 3'-end of AhR, ESR1, GR, CCND1, PTEN mRNA. Thus, the expression profile of miR-21, -221, -222, -143, -152 might change under the xenobiotics exposure. In silico analysis confirmed, that microRNAs target not only cytochrome P450 mRNA but also other genes, including those involved in hormonal carcinogenesis, they also can be regulated with studied miRs

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Effect of benzo[a]pyrene on the expression of miR‐126, miR‐190a and their target genes EGFL7, TP53INP1 and PHLPP1 in primary endometrial cells

Чанышев

Koval

Nushtaeva

et al. 2019

J Biochem & Molecular Tox

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The main topic of this study was to investigate the effect of benzo[a]pyrene (BP) on microRNAs and their target genes expression levels in primary cell cultures from normal and malignant endometrial tissue. MicroRNA-126 (miR-126) and miR-190a were most sensitive to BP treatment. The treatment of both cultures with BP was accompanied by a decrease of miR-126 level and an increase of EGFL7 gene expression level. BP-induced upregulation of miR-190a was detected only in normal cells and it was accompanied with decrease of mRNA levels of TP53INP1 and PHLPP1 genes. Taking into account that BP promoted the proliferation of normal cells and amplified apoptosis of cancer cells, it is possible that miR-190a is involved in general cellular response to BP. The findings of this study indicate that miR-190a and its target genes may be involved in the regulation of cell fate under BP treatment.

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Expression of microRNAs, CYP1A1 and CYP2B1 in the livers and ovaries of female rats treated with DDT and PAHs

Cited by 32 publications

References 21 publications

Application of talcum powder, trichloroacetic acid and silver nitrate in female rats for non‐surgical sterilization: evaluation of the apoptotic pathway mRNA and miRNA genes

Application of talcum powder, trichloroacetic acid and silver nitrate in female rats for non‐surgical sterilization: evaluation of the apoptotic pathway mRNA and miRNA genes

Effect of xenobiotics on microrna expression in rat liver

Effect of benzo[a]pyrene on the expression of miR‐126, miR‐190a and their target genes EGFL7, TP53INP1 and PHLPP1 in primary endometrial cells

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