Expression of MCSP and PRAME in conjunctival melanoma

Westekemper, Henrike; Karimi, Sara; Süßkind, Daniela; Anastassiou, Gerassimos; Freistühler, Michael; Meller, Daniel; Zeschnigk, Michael; Steuhl, Klaus‐Peter; Bornfeld, Norbert; Schmid, K. W.; Grabellus, Florian

doi:10.1136/bjo.2009.167445

Cited by 25 publications

(42 citation statements)

References 37 publications

(24 reference statements)

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“…Tissue preparation and immunohistological staining have been performed as described before 21. As a pretreatment for Bcl-2-staining, EDTA buffer (Zytomed Systems, Germany, ZUC029-500) was applied.…”

Section: Methodsmentioning

confidence: 99%

Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma

Westekemper

Karimi

Süßkind

et al. 2010

British Journal of Ophthalmology

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Section: Methodsmentioning

confidence: 99%

Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma

Westekemper

Karimi

Süßkind

et al. 2010

British Journal of Ophthalmology

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Section: Imaging and Identification Of Intracellular Melaninmentioning

confidence: 99%

“…Confocal PTM was used in combination with fluorescence microscopy to assess the correlation between pigmentation of individual cells and expression of MCSP, which is implicated in cell adhesion, motility, and invasion processes (49,50). Although MCSP expression has been linked to melanoma pigmentation and neoplastic phenotype, no data have been presented to date that show a clear relationship between pigmentation and oncogenesis or metastatic potential (49). The accuracy of pigmentation-based prognosis could be impaired if histological pigmentation is assessed at low sensitivity, or if metastatic activity is determined by a minor subset of circulating melanoma cells (51), or if pigmentation is affected by conditions at each site of metastatic growth (52).…”

Section: Imaging and Identification Of Intracellular Melaninmentioning

confidence: 99%

Photothermal Confocal Spectromicroscopy of Multiple Cellular Chromophores and Fluorophores

Nedosekin

Galanzha

Ayyadevara

et al. 2012

Biophysical Journal

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Confocal fluorescence microscopy is a powerful biological tool providing high-resolution, three-dimensional (3D) imaging of fluorescent molecules. Many cellular components are weakly fluorescent, however, and thus their imaging requires additional labeling. As an alternative, label-free imaging can be performed by photothermal (PT) microscopy (PTM), based on nonradiative relaxation of absorbed energy into heat. Previously, little progress has been made in PT spectral identification of cellular chromophores at the 3D microscopic scale. Here, we introduce PTM integrating confocal thermal-lens scanning schematic, time-resolved detection, PT spectral identification, and nonlinear nanobubble-induced signal amplification with a tunable pulsed nanosecond laser. The capabilities of this confocal PTM were demonstrated for high-resolution 3D imaging and spectral identification of up to four chromophores and fluorophores in live cells and Caenorhabditis elegans. Examples include cytochrome c, green fluorescent protein, Mito-Tracker Red, Alexa-488, and natural drug-enhanced or genetically engineered melanin as a PT contrast agent. PTM was able to guide spectral burning of strong absorption background, which masked weakly absorbing chromophores (e.g., cytochromes in the melanin background). PTM provided label-free monitoring of stress-related changes to cytochrome c distribution, in C. elegans at the single-cell level. In nonlinear mode ultrasharp PT spectra from cyt c and the lateral resolution of 120 nm during calibration with 10-nm gold film were observed, suggesting a potential of PTM to break through the spectral and diffraction limits, respectively. Confocal PT spectromicroscopy could provide a valuable alternative or supplement to fluorescence microscopy for imaging of nonfluorescent chromophores and certain fluorophores.

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“…We have observed strong expression of PRAME (preferentially expressed antigen of melanoma, a CT antigen family member) in uveal melanoma cell lines (mRNA and protein) (M Madigan, personal communication, 2010). Conjunctival melanomas and lymph node metastases were also recently reported to express high levels of PRAME with implications for retinoic acid-based therapies 35. The potential for epigenetic modulation of CT antigen expression has also been reported, with de novo induction of CT antigens following treatment of tumour cells with hypomethylating agents such as 5-aza-2′-deoxycytidine (5AC) 36.…”

Section: Discussionmentioning

confidence: 99%

Expression of cancer-testis antigens (MAGE-A1, MAGE-A3/6, MAGE-A4, MAGE-C1 and NY-ESO-1) in primary human uveal and conjunctival melanoma

et al. 2011

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Uveal and conjunctival melanomas consistently expressed high levels of the differentiation antigens (gp100, Melan-A/MART1 and tyrosinase). However, compared with other tumours, including cutaneous melanoma, only low levels of CT antigens were found in ocular melanomas. These observations suggest that immunotherapy directly targeting the CT antigens studied may not be effective for ocular melanoma.

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Expression of MCSP and PRAME in conjunctival melanoma

Cited by 25 publications

References 37 publications

Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma

Expression of HSP 90, PTEN and Bcl-2 in conjunctival melanoma

Photothermal Confocal Spectromicroscopy of Multiple Cellular Chromophores and Fluorophores

Expression of cancer-testis antigens (MAGE-A1, MAGE-A3/6, MAGE-A4, MAGE-C1 and NY-ESO-1) in primary human uveal and conjunctival melanoma

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