Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements

Abstract

Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), Biodentine TM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.

Matrix Metalloproteinases in Dental and Periodontal Tissues and Their Current Inhibitors: Developmental, Degradational and Pathological Aspects

“…Higher levels of MMP-8 are found in irreversible pulpitis with higher pain scores [ 58 , 59 ], explicitly expressed by polymorphonuclear leukocytes, macrophages, plasma cells and some endothelial cells of the blood vessels of the pulp tissue proper, suggesting the role of MMP-8 in extracellular matrix degradation during pulp and periapical tissue inflammation. The level of MMP-8 progressively decreases after 15 days of a mineral trioxide aggregate (MTA) pulpotomy procedure in rat molars, significantly more than Biodentine and calcium hydroxide pulpotomies, indicating the superiority of MTA for vital pulp therapy [ 60 ].…”