Expression of matrix metalloproteinase 1, matrix metalloproteinase 2, and matrix metalloproteinase 9 in carcinoma of the head and neck

Franchi, Alessandro; Santucci, Marco; Masini, Emanuela; Sardi, Iacopo; Paglierani, Milena; Gallo, Oreste

doi:10.1002/cncr.10916

Cited by 131 publications

(145 citation statements)

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“…Due to lack of data, however, nine articles had to be excluded from the current metaanalysis. The remaining 5 publications with a total of 238 patients 7,9,[17][18][19] demonstrated MMP-9 expression in 150 tissue specimens. The common odds ratio is summarized in Table 2.…”

Section: Results For Mmp-9mentioning

confidence: 99%

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Metaanalysis of the significance of matrix metalloproteinases for lymph node disease in patients with head and neck squamous cell carcinoma

Wiegand

Dünne

Müller

et al. 2005

Cancer

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Objective: To study the reliability of the sentinel node technique (SNT) in the management of the N0 neck in patients with supraglottic laryngeal cancer. Methods: Patients presenting to our department, between 2001 and 2004, with T1–T3 supraglottic cancer and N0 neck, clinically and radiologically, were included in the study. All patients underwent sentinel lymph node detection, using the hand‐held gamma probe followed by bilateral modified radical neck dissection (MRND) and resection of the primary tumor. We compared the results of the SNT with those of the gold standard MRND for the staging of the neck. The histologic examination was performed after fixation for both the products of SNT and MRND. Results: Twenty‐nine patients were included in the study. Twenty‐one had T2, 2 had T1, and 6 had T3 tumors. Patients had transoral CO2 laser‐assisted resection of the primary tumor. All the patients underwent bilateral neck dissection immediately after the sentinel node biopsy. Of the 95 sentinel lymph nodes harvested, 22 were positive for lymph node metastasis. The mean number of sentinel node per patient was 3. Of the 58 modified neck dissections (2 neck sides/patient), there was 1 additional positive lymph node, in the prelaryngeal area, and the patient had already positive sentinel lymph nodes. The SNT allowed the identification of node metastasis in 100% of the cases with a sensitivity of 100 (48–100), specificity of 78 (64–88), negative predictive value of 100 (91–100). Conclusions: SNT is reliable in the staging of the clinically N0 neck in patients with supraglottic cancer. Laryngoscope, 2010

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Section: Results For Mmp-9mentioning

confidence: 99%

“…cN status and pN status were considered in two publications, 7,17 whereas one publication considered only pN status, 18 and two other publications 9,19 did not provide any information about lymph node status. None of the studies mentioned compared levels of MMP-expression with cN status or pN status.…”

Section: Results For Mmp-9mentioning

confidence: 99%

Metaanalysis of the significance of matrix metalloproteinases for lymph node disease in patients with head and neck squamous cell carcinoma

Wiegand

Dünne

Müller

et al. 2005

Cancer

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“…26 These data indicate that poorly differentiated oral cancer in Taiwan is significantly lower than the report of 21-42.8% in the Western countries. 8,27 It is unknown if different location of the oral cancer, the different etiology or different races may account for the observed difference in the percentage of cell types. MMP-7, also known as matrilysin-1, is the smallest member of the MMP family, 15,28 as it lacks the C-terminal hemopexin domain common to other MMPs.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of MMPs in head and neck cancers has been reported. [4][5][6][7][8][9][10][11][12][13][14] Those studies demonstrated that immunoreactive MMP-2 and MMP-9 staining was stronger in patients with lymph node metastasis and was associated with lower survival rates. [11][12][13][14] MMP-7 is one of the few MMPs overexpressed by carcinoma cells rather than by stromal cells.…”

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confidence: 99%

Active matrix metalloproteinase-7 is associated with invasion in buccal squamous cell carcinoma

Chuang

Huang

et al. 2008

Modern Pathology

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Protein microarrays have shown that matrix metalloproteinase-7 is upregulated in head and neck squamous cell carcinomas, but its role in local tissue invasion is still uncertain. We investigated the expression of active matrix metalloproteinase-7, using tissue microarray, immunohistochemistry, and western blotting, in oral tissues from 24 patients with buccal squamous cell carcinoma, and correlated the findings with clinicopathological features. Normal buccal tissue samples from the same patients, obtained at sites at least 1 cm from tumor tissue, served as normal controls. Total matrix metalloproteinase-7 was detected on western blots in 9 of 15 (60%) tumor tissue samples and in 2 of 15 (13%) normal mucosal samples; this difference was significant (P ¼ 0.008). Moreover, the active matrix metalloproteinase-7 was expressed only in eight of the nine (89%) tumor samples that expressed matrix metalloproteinase-7, and in none of the normal tissue samples, regardless of the expression status of the pro-matrix metalloproteinase-7. Immunostaining of matrix metalloproteinase-7 was observed histologically in both tumor and nonneoplastic epithelium, but immunostaining of active matrix metalloproteinase-7 was present only in tumor nests. Expression of active matrix metalloproteinase-7 was associated with larger tumor size (P ¼ 0.022) and was significantly higher in buccal squamous cell carcinoma with adjacent skin or bone invasion (P ¼ 0.036). In conclusion, active matrix metalloproteinase-7 expression was associated with more aggressive buccal squamous cell carcinomas.

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“…5 Overexpression of MMP1 has been demonstrated in HNSCC tissues and has been found to be associated with poor outcome. 3,[6][7][8] The expression of MMP1 is partly regulated by its upstream promoter sequences. 9 Recently, a functional single nucleotide polymorphism (SNP) in the promoter region of the human MMP1 has been reported.…”

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confidence: 99%

A functional polymorphism in the matrix metalloproteinase‐1 gene promoter is associated with susceptibility and aggressiveness of head and neck cancer

O-charoenrat

Leksrisakul

Sangruchi

2006

Intl Journal of Cancer

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Matrix metalloproteinases (MMPs) play an important role in several steps of cancer development. A single guanine insertion polymorphism (2G) in the MMP1 promoter sequence at 21,607 creates an Ets binding site and thus results in enhancing transcriptional activity. This study aimed to evaluate the contribution of this 2G polymorphism on susceptibility and aggressiveness of HNSCC. A panel of HNSCC cell lines and peritumoral fibroblasts were examined for the MMP1 genotypes and expression levels. Genomic DNA was extracted from peripheral blood of 300 patients with newly diagnosed HNSCC and from 300 age-and gender-matched cancer-free controls. Genotyping was carried out using a PCR-RFLP assay. The levels of MMP1 mRNA expression were evaluated by the quantitative RT-PCR and a correlation with different genotype was determined. Odds ratio (OR) for cancer risk were calculated using multivariate logistic regression. In addition, a correlation between the 2G/2G genotype and clinicopathological parameters was examined. Eleven out of 18 HNSCC cell lines showed the 2G/2G genotype (61%) and only 1 cell line had the 1G/1G genotype (5.6%). Cell lines with the 2G/2G genotype expressed significantly higher mean MMP1 mRNA level than those with other genotypes. In clinical model, subjects carrying the homozygous 2G/2G genotype had a higher risk of head and neck cancer compared with subjects with other genotypes (adjusted OR: 2.28; 95% CI: 1.58-3.27), controlling for major confounders. A correlation between promoter polymorphisms and the levels of MMP1 expression in cancer tissues was found, and this 2G/2G genotype was correlated with the adverse clinicopathological parameters. Finally, the highest level of MMP1 enhancement was demonstrated in the coculture of tumor cells and peritumoral fibroblasts of 2G homozygotes. These findings suggest that the presence of 2G polymorphism at the MMP1 promoter is associated with the development and progression of HNSCC. ' 2005 Wiley-Liss, Inc.Key words: head and neck cancer; squamous cell carcinoma; matrix metalloproteinase; single nucleotide polymorphism Head and neck squamous cell carcinoma (HNSCC) is 1 of the 5 most common cancers worldwide. 1 In Thailand, HNSCC accounts for 1 in 4 cancers in men and is the fourth most common cancer in women. 2 Although smoking and alcohol use play a major role in the etiology of these malignancies, only a fraction of smokers and drinkers develop this disease, suggesting a genetic susceptibility in the general population. In addition, the inherited genetic factors in some HNSCC patients may be responsible for a more aggressive disease. The ability to identify such high-risk individuals will have major influence in the cancer prevention and treatment programs in the future.The matrix metalloproteinases (MMPs), a family of 23 extracellular endopeptidases with enzymatic activity against almost all protein components of the extracellular matrix (ECM), have been implicated in the invasion and metastasis of HNSCC. 3 Recent work has suggested that, in addition to pr...

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Expression of matrix metalloproteinase 1, matrix metalloproteinase 2, and matrix metalloproteinase 9 in carcinoma of the head and neck

Cited by 131 publications

References 45 publications

Metaanalysis of the significance of matrix metalloproteinases for lymph node disease in patients with head and neck squamous cell carcinoma

Metaanalysis of the significance of matrix metalloproteinases for lymph node disease in patients with head and neck squamous cell carcinoma

Active matrix metalloproteinase-7 is associated with invasion in buccal squamous cell carcinoma

A functional polymorphism in the matrix metalloproteinase‐1 gene promoter is associated with susceptibility and aggressiveness of head and neck cancer

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