Active matrix metalloproteinase-7 is associated with invasion in buccal squamous cell carcinoma

Abstract

Protein microarrays have shown that matrix metalloproteinase-7 is upregulated in head and neck squamous cell carcinomas, but its role in local tissue invasion is still uncertain. We investigated the expression of active matrix metalloproteinase-7, using tissue microarray, immunohistochemistry, and western blotting, in oral tissues from 24 patients with buccal squamous cell carcinoma, and correlated the findings with clinicopathological features. Normal buccal tissue samples from the same patients, obtained at sites at least 1 cm from tumor tissue, served as normal controls. Total matrix metalloproteinase-7 was detected on western blots in 9 of 15 (60%) tumor tissue samples and in 2 of 15 (13%) normal mucosal samples; this difference was significant (P ¼ 0.008). Moreover, the active matrix metalloproteinase-7 was expressed only in eight of the nine (89%) tumor samples that expressed matrix metalloproteinase-7, and in none of the normal tissue samples, regardless of the expression status of the pro-matrix metalloproteinase-7. Immunostaining of matrix metalloproteinase-7 was observed histologically in both tumor and nonneoplastic epithelium, but immunostaining of active matrix metalloproteinase-7 was present only in tumor nests. Expression of active matrix metalloproteinase-7 was associated with larger tumor size (P ¼ 0.022) and was significantly higher in buccal squamous cell carcinoma with adjacent skin or bone invasion (P ¼ 0.036). In conclusion, active matrix metalloproteinase-7 expression was associated with more aggressive buccal squamous cell carcinomas.

Insulin‐like growth factor II mRNA‐binding protein 3: A novel prognostic biomarker for oral squamous cell carcinoma

“…The sites of OSCC occurrence in patients from the East are different from those in the West. 18 The predominant sites in Korea are gingivae and tongue, according to our statistics (Table 1). Moreover, clinicopathologic factors including histopathologic grade and TNM classification can provide some prognostic information in patients with OSCC, although detection of local deep invasion and potential metastases remains limited.…”

Potential molecular targets for inhibiting bone invasion by oral squamous cell carcinoma: a review of mechanisms

“…Using immunohistochemistry and flow cytometry, they also detected high levels of expression of extracellular matrix metalloproteinase inducer in these cells. Another research group found that tumour tissues from 9 out of 24 cases of buccal SCC with mandibular invasion were stained strongly for active MMP-7, compared to 15 cases without invasion of the adjacent bone [25]. This is the first study demonstrating that MMP-7 is correlated with mandibular invasion, a significant observation which is consistent with the demonstration by others that secretion of MMP-7 by prostate and breast cancer cells is capable of cleaving the receptor activator of nuclear factor-κB ligand (RANKL) to an active soluble form, which then induces osteoclast formation and bone resorption [26,27].…”

Association of MMP7 −181A→G Promoter Polymorphism with Gastric Cancer Risk

“…Accumulated evidence showed a positive correlation between MMP7 expression and the invasive potential of cancers (18,19). Previous study demonstrated that knockdown or overexpression of MMP7 alters tumorigenicity.…”

“…Like other MMPs, the activity of MMP7 is regulated at multiple levels, including gene expression, spatial localization, zymogen activation, and inhibition by its endogenous inhibitor (14,17). In contrast to other MMPs, which usually are expressed in stromal tissues, MMP7 is expressed mainly in tumor cells (18,19) and enhances tumor progression by inhibiting apoptosis in cancer cells, by reducing cell adhesion, or by inducing angiogenesis or both (20).…”