Expression of lncRNAs in response to bacterial infections of goat mammary epithelial cells reveals insights into mammary gland diseases

Mumtaz, Peerzada Tajamul; Taban, Qamar; Bhat, Basharat Ahmad; Ahmad, Syed Mudasir; Dar, Muneer Ahmad; Kashoo, Zahid Amin; Ganie, Nisar Ahmad; Shah, Rehan Ali

doi:10.1016/j.micpath.2021.105367

Cited by 10 publications

(9 citation statements)

References 69 publications

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“…The sequencing was performed at SciGenom Lab (Cochin, India) using Illumina HiSeq 2500. RNA-Seq data were filtered and analyzed as per the pipeline described previously (48, 49).…”

Section: Methodsmentioning

confidence: 99%

“…These results indicate that SCARB1 regulates the production of proinflammatory mediators in GMECs in response to E. coli. The expression values of SCARB1 for control vs. E.coli infected samples were measured as fragments per kilobase of exon per million fragments mapped (FPKM) using Cufflinks v2.1.1 package with absolute log2 (fold change) of -0.050414 (49).…”

Section: Gmecsmentioning

confidence: 99%

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Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Taban

Ahmad

Mumtaz

et al. 2022

Preprint

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SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host-pathogen interactions by acting in co-ordination with Toll-like receptors. Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease- esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.

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“…The sequencing was performed at SciGenom Lab (Cochin, India) using Illumina HiSeq 2500. RNA-Seq data were filtered and analyzed as per the pipeline described previously (48, 49).…”

Section: Methodsmentioning

confidence: 99%

Section: Gmecsmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Taban

Ahmad

Mumtaz

et al. 2022

Preprint

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“…Compared with advances in the study of lncRNAs in the regulation of inflammatory diseases in humans and mice, the study of lncRNAs in the regulation mechanism of mastitis has been slow. Mumtaz et al [ 134 ] identified 112 differentially expressed lncRNAs in goat mammary epithelial cells induced by E. coli and S. aureus . In addition, Wang et al [ 135 ] also found 112 differentially expressed lncRNAs in LPS-treated bovine mammary epithelial cells, and these lncRNAs participated in inflammation-related signal pathways (i.e., the Notch, NF-κB, MAPK, and PI3K-AKT signal pathways).…”

Section: Role Of Lncrnas In Inflammatory Diseasesmentioning

confidence: 99%

Role of Long Noncoding RNAs in the Regulation of Cellular Immune Response and Inflammatory Diseases

Feng

Jiao

Wang

et al. 2022

Cells

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Long noncoding RNAs (lncRNAs) are recently discovered genetic regulatory molecules that regulate immune responses and are closely associated with the occurrence and development of various diseases, including inflammation, in humans and animals. Under specific physiological conditions, lncRNA expression varies at the cell or tissue level, and lncRNAs can bind to specific miRNAs, target mRNAs, and target proteins to participate in certain processes, such as cell differentiation and inflammatory responses, via the corresponding signaling pathways. This review article summarizes the regulatory role of lncRNAs in macrophage polarization, dendritic cell differentiation, T cell differentiation, and endothelial and epithelial inflammation. In addition, it describes the molecular mechanism of lncRNAs in acute kidney injury, hepatitis, inflammatory injury of the lung, osteoarthritis, mastitis, and neuroinflammation to provide a reference for the molecular regulatory network as well as the genetic diagnosis and treatment of inflammatory diseases in humans and animals.

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“…Long noncoding RNAs (lncRNAs) are transcripts of more than 200 nucleotides in length that can reprogram gene expression and affect different cell functions, including cell fate determination, cell cycle progression, apoptosis, and senescence. , Previous studies have shown that lncRNAs are involved in mammary gland development, lactation, and mastitis. − The lncRNA NEAT1 deficiency in mice is associated with decreased proliferation of mammary epithelial cells, resulting in the degeneration of mammary duct morphology and lactation function in mice . lncROPM binds to the 3′ UTR of the PLA 2 G 16 gene and increases the stability of mRNA, thereby activating the Wnt/β-catenin, Hippo/YAP, and PI3K/AKT signaling pathways and ultimately participating in the maintenance of breast cancer stem cells …”

Section: Introductionmentioning

confidence: 99%

lncRNA MPFAST Promotes Proliferation and Fatty Acid Synthesis of Bovine Mammary Epithelial Cell by Sponging miR-103 Regulating PI3K-AKT Pathway

Liu

Sun

Zhang

et al. 2022

J. Agric. Food Chem.

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Long noncoding RNAs (lncRNAs) have an essential role in mammary gland development and lactation. Our earlier study showed that the lncRNA mammary proliferation and fatty acid synthesis-associated transcript (MPFAST) is highly expressed in the Holstein cow mammary gland during the middle lactation period compared to the dry period, which indicates its potential role in lactation. Therefore, gain- and loss-of-function experiments were performed on bovine mammary epithelial cells (BMECs) by cell counting kit 8 (CCK8), 5-ethynyl-2′-deoxyuridine (EdU), real-time quantitative polymerase chain reaction (RT-qPCR), and western blot. The results indicated that MPFAST promoted the viability and proliferation of BMECs. The oil red O staining and cellular triglyceride assay further showed that MPFAST promoted the number of lipid droplets and cellular triglyceride synthesis in BMECs. Bioinformatics analysis showed that MPFAST could act as a molecular sponge for miR-103, and PIK3R1 was a potential target of miR-103, which was further confirmed by the dual-luciferase reporter assay, RT-qPCR, and western blot. The overexpression of MPFAST promoted the expression of PIK3R1 at mRNA and protein levels. It also significantly increased the mRNA relative expression levels of AKT, mTOR, and SREBP1, and the protein relative expression levels of AKT and p-AKT in the PI3K-AKT signaling pathway. In contrast, the inhibition of MPFAST resulted in the downregulation of the PI3K-AKT signaling pathway genes. These results indicated that MPFAST regulates the expression of the genes in the PI3K-AKT signaling pathway through sponging miR-103 and promotes the proliferation and synthesis of fatty acids of BMECs. Our results would provide a new direction for further exploring the regulatory mechanism of lncRNA in the mammary gland.

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Expression of lncRNAs in response to bacterial infections of goat mammary epithelial cells reveals insights into mammary gland diseases

Cited by 10 publications

References 69 publications

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Role of Long Noncoding RNAs in the Regulation of Cellular Immune Response and Inflammatory Diseases

lncRNA MPFAST Promotes Proliferation and Fatty Acid Synthesis of Bovine Mammary Epithelial Cell by Sponging miR-103 Regulating PI3K-AKT Pathway

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