Expression of lipoxygenase isoenzymes in developing barley grains

Schmitt, Nathalie; Mechelen, J.R. van

doi:10.1016/s0168-9452(97)00152-0

Cited by 18 publications

(18 citation statements)

References 19 publications

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“…It should be noted that 9-LOX is the major LOX in the embryo of quiescent barley seeds (42,43) and that AOS is the major enzyme metabolizing lipoxygenase-generated hydroperoxides in embryos of quiescent barley grains (41). The very efficient trapping of 9,10-allene oxide by 50 M LTP1 in an aqueous environment (about 55 M water) (Fig.…”

Section: Discussionmentioning

confidence: 99%

Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

Bakan

Hámberg

Perrocheau

et al. 2006

Journal of Biological Chemistry

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Lipid transfer proteins (LTPs) are ubiquitous plant lipidbinding proteins that have been associated with multiple developmental and stress responses. Although LTPs typically bind fatty acids and fatty acid derivatives in a non-covalent way, studies on the LTPs of barley seeds have identified an abundantly occurring covalently modified form, LTP1b, the lipid ligand of which has resisted clarification. In the present study, this adduct was identified as the ␣-ketol 9-hydroxy-10-oxo-12(Z)-octadecenoic acid. Further studies on the formation of LTP1b demonstrated that the ligand was introduced by nucleophilic attack of the free carboxylate group of the Asp-7 residue of the protein at carbon-9 of the allene oxide fatty acid 9(S),10-epoxy-10,12(Z)-octadecadienoic acid. This reactive oxylipin was produced in barley seeds by oxygenation of linoleic acid by 9-lipoxygenase followed by dehydration of the resulting hydroperoxide by allene oxide synthase. The generation of protein-oxylipin adducts represents a new function for plant allene oxide synthases, enzymes that have earlier been implicated mainly in the biosynthesis of the jasmonate family of plant hormones. Additionally, the LTP-allene oxide synthase interaction opens new perspectives regarding the roles of LTPs in the signaling of plant defense and development.

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Section: Discussionmentioning

confidence: 99%

Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

Bakan

Hámberg

Perrocheau

et al. 2006

Journal of Biological Chemistry

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“…First, transcript levels of the barley LoxB gene (orthologue of wheat Lpx-3) are detected mainly in germinating grains and are extremely low during grain development compared with LoxA and LoxC (Schmitt and van Mechelen, 1997;van Mechelen et al, 1999). On the contrary, LoxA (orthologue of wheat Lpx-1) accounts for most of the total LOX activity in mature barley grains (Schmitt and van Mechelen, 1997) and therefore, is the one that can affect pigments during pasta processing.…”

Section: Characterization Of Loci Affecting Color and Lox Activity Inmentioning

confidence: 99%

“…On the contrary, LoxA (orthologue of wheat Lpx-1) accounts for most of the total LOX activity in mature barley grains (Schmitt and van Mechelen, 1997) and therefore, is the one that can affect pigments during pasta processing. A second line of evidence supporting the more important role of the Lpx-1 gene on LOX activity was the discovery of a deletion in the Lpx-B1.1 locus in the parent with low LOX activity (Kofa).…”

Section: Characterization Of Loci Affecting Color and Lox Activity Inmentioning

confidence: 99%

“…LOX-1 is present in both quiescent and germinating barley grains and accounts for most of the LOX activity in mature barley grains. LOX-2 is detected only at early stages of grain development and after germination (Holtman et al, 1996;Schmitt and van Mechelen, 1997;van Mechelen et al, 1999). The LOX-1 isoform is encoded by the LoxA gene (GenBank L35931) located in chromosome arm 4HS, whereas the LOX-2 isoform is encoded by the LoxC gene (GenBank L37358) mapped on chromosome arm 5HL (van Mechelen et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…A third LOX cDNA, LoxB (GenBank L37359), was mapped 1-cM distal to the LoxA locus on barley chromosome arm 4HS (van Mechelen et al, 1999). LoxB mRNAs were mainly detected in germinating seeds and at much lower levels than the other two genes in the developing barley grains, and only at the onset of development (Schmitt and van Mechelen, 1997;van Mechelen et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

Carrera

Echenique

Zhang

et al. 2007

Journal of Cereal Science

115

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The concentration of yellow carotenoid pigments in durum wheat grain is an important quality criterion and is determined both by their accumulation and by their degradation by lipoxygenase enzymes (Lpx loci). The existence of a duplication at the Lpx-B1 locus and the allelic variation for a deletion of the Lpx-B1.1 copy is reported. This deletion was associated with a 4.5-fold reduction in lipoxygenase activity and improved pasta color (Po0.0001) but not semolina color, suggesting reduced pigment degradation during pasta processing. A molecular marker for the deletion was mapped on chromosome 4B in a population obtained from the cross between durum line UC1113 and variety Kofa. A second lipoxygenase locus, designated Lpx-A3, was mapped on the homoeologous region on chromosome 4A and was associated with semolina and pasta color (Po0.01) but not with lipoxygenase activity in the mature grain. Selection for both the UC1113 allele for Lpx-A3 and the Kofa Lpx-B1.1 deletion resulted in a 10% increase in yellow scores for dry pasta relative to the opposite allele combination. This result indicates that the markers and the new allelic variants reported here will be useful tools to manipulate the wheat Lpx loci and to improve pasta color. r

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Chemical Composition in Barley Grains and Malt Quality

Fox

2009

Advanced Topics in Science and Technology in China

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Expression of lipoxygenase isoenzymes in developing barley grains

Cited by 18 publications

References 19 publications

Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

A deletion at the Lpx-B1 locus is associated with low lipoxygenase activity and improved pasta color in durum wheat (Triticum turgidum ssp. durum)

Chemical Composition in Barley Grains and Malt Quality

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