Expression of lectin and defensin genes in Mironovskaya 808 and Roazon wheat cultivars infected with Pseudocercosporella herpotrichoides

Abstract

The dynamics of expression of protective proteins (lectin and defensin) genes of different genotypes of the winter wheat, affected by P. herpotrichoides, was investigated. Methods. RT-PCR was used for gene expression analysis. Results. We established that the lectin mRNA content in control and infecting plants of the resistant cultivar is considerably higher than in the susceptible one; the character of defensin mRNA accumulation in the investigated cultivars, control and infecting plants, does not change. Conclusion. The spare pool of lectin mRNA in the resistant cultivar is more substantial, than in the susceptible one, that is, possibly, one of the factors of the plant resistance. Inability to support constantly a high level of the protective genes expression seems to influence negatively on the cultivar resistance .

The Effect of Silver and Copper Nanoparticles on the Wheat—Pseudocercosporella herpotrichoides Pathosystem

“…The inoculation was performed under sterile conditions. The cultivation of P. herpotrichoides mycelium was conducted at 25 ± 1 °C for 14 days [4]. …”

Differential expression of cytochrome oxidase and ALY-family genes in resistant and susceptible tomato cultivars (Solanum lycopersicum) inoculated with Tomato bushy stunt virus

“…3). Belava et al [2] reported that resistance of plants to pathogens is determined by complicated interdependent systems, which are genetically controlled. The obtained transcripts of the PR-proteins (PR-1.1 and PR-1.2) exhibited a rapid up-regulation as early as 4 days post-inoculation, reached maximum levels after 16 days followed by downregulation after 32 days.…”

Photosynthetic membranes glycolipids of different wheat (Triticum aestivum) varieties under biotic stressor action

“…3. Вміст СХДГ в проростках пшениці за інфікування P. herpotrichoides Суттєве зниження вмісту сульфоліпідів впродовж експерименту в проростках сорту Roazon (від 81 % до 30 %), може бути зумовлене інтенсивним використанням сульфуру для утворення специфічних пептидів, а саме захисних білків -лектинів та дефенсинів, індукція яких, за отриманими нами попередньо даними [22], відбувається на початку патогенезу та супроводжується накопиченням відповідних мРНК.…”