Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin)

Abstract

ABSTRACT:In recent times, attention has focused on immunostimulants and plant products which could have beneficial effects in disease control. At present, the application of immunostimulants has been considered a more effective approach to health management in aquaculture through the enhancement of immune capability and disease resistance in shrimp. Garlic possesses bactericidal property against bacteria and can inhibit the growth of protozoa as well as gregarine infection in cultured shrimp. However, its effect on viral disease infection has not been well studied and requires further investigation. Garlic can also stimulate shrimp haemocytes as determined by cellular immune responses (phagocytic activity, superoxide anion production and phenoloxidase activity) suggesting that the immune-stimulatory properties of garlic will be useful for improvement of shrimp health. Here, we determined the expression of the immune-related genes Penaeidin, Crustin, Lysozyme, Toll-like, and tumour necrosis factor in kuruma shrimp, upon stimulation with allicin extract. The expression of these factors was measured for the first time and was found to be elevated in intestine and lymphoid organ after in vivo stimulation for 3, 12, 24 and 48 h. We conclude that garlic can be used in shrimp culture as an alternative to antibiotics or chemotherapeutic agents; however, further research is needed under field conditions.

Effects of Dietary Supplementation of Spirulina platensis and Garlic on the Growth Performance and Expression Levels of Immune-related Genes in Nile tilapia (Oreochromis niloticus)

“…Garlic treated group showed significant upregulation in TNFα pre/post bacterial infection [58]. Sung et al [57] found that although garlic has increased LPS-induced TNF-α.…”

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

“…xidant enzyme activities of shrimp against V. alginolyti cus and WSSV infection (Wu et al 2015). The purified garlic compounds allicin and ajoene demonstrated immune stimulant capacity against fish pathogenic protozoa Spironucleus vortens and Ichthyophthirius multifiliis, and the bacteria A. hydrophila (Nya et al 2010;Tanekhy and Fall 2016). Recently, Foysal et al (2019) reported that dietary administration of garlic could modulate gut microbiota, increase recovery from streptococcus infection and upregulate the expression of immune genes in the intestinal tissue of tilapia.…”

Isolation and identification of Enterococcus faecalis from cultured Oreochromis niloticus and Mugil cephalus with a special emphasis on a possible integrated control strategy

“…As “prevention is better than treatment urged scientists to accept alternative management strategies such as using plant products in aquaculture, which is one of the promising alternatives to antibiotics, whereas, they stimulate the fish immune system, prevent stress and act as antimicrobial agents due to their active chemical ingredients (Assefa & Abunna, 2018). At present, many scientific literature has considered the potential use and adoption of several synthetic and/or organic plant derivatives for the treatment of aquaculture diseases, including synthetic polyphenol compound (Tinh et al, 2021), Mentha piperita peppermint (Talpur, 2014), Origanum vulgare L. (Abdel‐Latif et al, 2020; Mabrok & Wahdan, 2018) and garlic Allium sativum (Tanekhy & Fall, 2015). Oregano essential oil (OEO) is believed to have remarkable antibacterial activity, which is mainly attributed to its phenolic components such as carvacrol and thymol.…”