Expression of Talaromyces thermophilus lipase gene in Trichoderma reesei by homologous recombination at the cbh1 locus

Zhang, Xu; Xia, Liming

doi:10.1007/s10295-016-1897-5

Cited by 16 publications

(7 citation statements)

References 46 publications

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“…RSJ-1, B. thermoleovorans ID-1, Bacillus LBN2, Cohnella sp. A01, B. licheniformis Ht7, and B. sonorensis 4R which were 67.0 kDa, 60.0 kDa, 59.0 kDa, 57.0 kDa, 50.0 kDa, 45.0 kDa, 40.0 kDa, 39.0 kDa, 37 kDa, 34.0 kDa, 33.0 kDa, 29.5 kDa, 22.0 kDa, and 21.87 kDa, respectively, as determined using sodium dodecyl sulfate- (SDS-) polyacrylamide gel electrophoresis (PAGE) [ 4 , 9 , 11 , 18 , 23 , 26 , 27 , 29 – 33 , 54 , 69 – 71 ].…”

Section: Characteristics Of Ta Lipasesmentioning

confidence: 99%

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Lajis

2018

Journal of Lipids

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For decades, microbial lipases are notably used as biocatalysts and efficiently catalyze various processes in many important industries. Biocatalysts are less corrosive to industrial equipment and due to their substrate specificity and regioselectivity they produced less harmful waste which promotes environmental sustainability. At present, thermostable and alkaline tolerant lipases have gained enormous interest as biocatalyst due to their stability and robustness under high temperature and alkaline environment operation. Several characteristics of the thermostable and alkaline tolerant lipases are discussed. Their molecular weight and resistance towards a range of temperature, pH, metal, and surfactants are compared. Their industrial applications in biodiesel, biodetergents, biodegreasing, and other types of bioconversions are also described. This review also discusses the advance of fermentation process for thermostable and alkaline tolerant lipases production focusing on the process development in microorganism selection and strain improvement, culture medium optimization via several optimization techniques (i.e., one-factor-at-a-time, surface response methodology, and artificial neural network), and other fermentation parameters (i.e., inoculums size, temperature, pH, agitation rate, dissolved oxygen tension (DOT), and aeration rate). Two common fermentation techniques for thermostable and alkaline tolerant lipases production which are solid-state and submerged fermentation methods are compared and discussed. Recent optimization approaches using evolutionary algorithms (i.e., Genetic Algorithm, Differential Evolution, and Particle Swarm Optimization) are also highlighted in this article.

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Section: Characteristics Of Ta Lipasesmentioning

confidence: 99%

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Lajis

2018

Journal of Lipids

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“…Lack of a generous cell wall facilitating DNA transfer makes filamentous fungi difficult to use in transformation experiments [49]. Owing to the complex processes involved in protoplast fusion, a research group found A. tumefaciens AGL1 to be efficient for the transformation of T-DNA to the host through a co-cultivation method and AS amendment [50]. Here, we applied the same method to transform the vp1 gene from A. tumefaciens AGL1 to T. reesei Rut-C30 with an efficiency of 250 to 400 successful transformants per 1 × 10 7 conidia.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering

et al. 2020

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Trichoderma reesei produces various saccharification enzymes required for biomass degradation. However, the lack of an effective lignin-degrading enzyme system reduces the species’ efficiency in producing fermentable sugars and increases the pre-treatment costs for biofuel production. In this study, we heterologously expressed the Ganoderma lucidum RMK1 versatile peroxidase gene (vp1) in the Rut-C30 strain of T. reesei. The expression of purified 6×His-tag–containing recombinant G. lucidum-derived protein (rVP1) was confirmed through western blot, which exhibited a single band with a relative molecular weight of 39 kDa. In saccharification and delignification studies using rice straw, the transformant (tVP7, T. reesei Rut-C30 expressing G. lucidum-derived rVP1) showed significant improvement in the yield of total reducing sugar and delignification, compared with that of the parent T. reesei Rut-C30 strain. Scanning electron microscopy (SEM) of tVP7-treated paddy straw showed extensive degradation of several layers of its surface compared with the parent strain due to the presence of G. lucidum-derived rVP1. Our results suggest that the expression of ligninolytic enzymes in cellulase hyperproducing systems helps to integrate the pre-treatment and saccharification steps that may ultimately reduce the costs of bioethanol production.

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“…Thus, this heterologous lipase is very promising for industrial applications like paper making and detergent addition (68). On the other hand the synergetic degradation of cellulose and lipase makes this modified microorganism an excellent candidate to the treatment of oil-rich mill waste treatment, where the cellulose and triglycerides coexist (69)(70).…”

Section: New Cell Factories Producing Recombinant Lipasesmentioning

confidence: 99%

Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review

Valero

2018

Lipases and Phospholipases

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The production of heterologous lipases is one of the most promising strategies to increase the productivity of the bioprocesses and to reduce costs, with the final objective that more industrial lipase applications could be implemented.In this chapter, an overview of the new success in synthetic biology, with traditional molecular genetic techniques and bioprocess engineering in the last 5 years in the cell factory Pichia pastoris, the most promising host system for heterologous lipase production, is presented.The goals get on heterologous Candida antarctica, Rhizopus oryzae, and Candida rugosa lipases, three of the most common lipases used in biocatalysis, are showed. Finally, new cell factories producing heterologous lipases are presented.

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Expression of Talaromyces thermophilus lipase gene in Trichoderma reesei by homologous recombination at the cbh1 locus

Cited by 16 publications

References 46 publications

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Realm of Thermoalkaline Lipases in Bioprocess Commodities

Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering

Recent Advances in Pichia pastoris as Host for Heterologous Expression System for Lipases: A Review

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