Expression of RSOsPR10 in rice roots is antagonistically regulated by jasmonate/ethylene and salicylic acid via the activator OsERF87 and the repressor OsWRKY76, respectively

Abstract

Plant roots play important roles in absorbing water and nutrients, and in tolerance against environmental stresses. Previously, we identified a rice root‐specific pathogenesis‐related protein ( RSO s PR 10) induced by drought, salt, and wounding. RSO s PR 10 expression is strongly induced by jasmonate ( JA )/ethylene ( ET ), but suppressed by salicylic acid ( SA ). Here, we analyzed the promoter activity of RSO s PR 10 . Analyses of transgenic rice lines harboring different‐length promoter:: β ‐glucuronidase ( GUS ) constructs showed that the 3‐kb promoter region is indispensable for JA / ET induction, SA repression, and root‐specific expression. In the JA ‐treated 3K‐promoter:: GUS line, GUS activity was mainly observed at lateral root primordia. Transient expression in roots using a dual luciferase ( LUC ) assay with different‐length promoter:: LUC constructs demonstrated that the novel transcription factor Os ERF 87 induced 3K‐promoter:: LUC expression through binding to GCC ‐ cis elements. In contrast, the SA ‐inducible Os WRKY 76 transcription factor strongly repressed the JA ‐inducible and Os ERF 87‐dependent expression of RSO s PR 10 . RSO s PR 10 was expressed at lower levels in Os WRKY 76 ‐overexpressing rice, but at higher levels in Os WRKY 76 ‐knockout rice, compared with wild type. These results show that two transcription factors, Os ERF 87 and Os WRKY 76, antagonistically regulate RSO s PR 10 expression through binding to the same promoter. This mechanism represents a fine‐tuning system to sense the balance between JA / ET and SA signaling in plants under environmental stress.

ERF Transcription Factor OsBIERF3 Positively Contributes to Immunity against Fungal and Bacterial Diseases but Negatively Regulates Cold Tolerance in Rice

“…The present study further revealed that OsBIERF3 had transcriptional activation activity in yeast and this activity depended on the C-terminal region ( Figure 2 ), demonstrating that OsBIERF3 is a transcriptional activator. This is similar to OsERF092, OsERF87, and OsERF136, which showed transcriptional activator activity in rice cells through binding to GCC box elements [ 48 , 112 ]. It is therefore reasonable to speculate that OsBIERF3 plays its role in immunity through activating downstream target genes that are involved in defense response and immune signaling.…”

“…This is similar to the expression induction of OsBIERF3 and OsERF092 in the incompatible and compatible interactions between rice and M. oryzae [ 46 , 48 ]. Furthermore, the expression of OsBIERF3 was induced by an SA analog benzothiadiazole [ 46 ], and by SA, MeJA, and ACC ( Figure 1 B), which is similar to OsERF083 and OsERF087 , which were induced by SA, JA, and ethephon [ 50 , 112 ]. Phytohormones such as SA, JA, and ET play critical roles in fine-tuning immunity in rice [ 113 , 114 , 115 ].…”

“…Biochemically, OsBIERF3, OsERF083, OsERF087, and OsERF092, were previously shown to bind to a canonical GCC box-containing sequence [ 46 , 48 , 50 , 112 ]. The present study further revealed that OsBIERF3 had transcriptional activation activity in yeast and this activity depended on the C-terminal region ( Figure 2 ), demonstrating that OsBIERF3 is a transcriptional activator.…”

Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools

“…JA and ET also induce RSOsPR10, while salicylic acid (SA) almost completely suppresses its induction [ 135 , 136 ]. JA-inducible and OsERF87-dependent expression of RSOsPR10 were strongly repressed by the SA-inducible OsWRKY76 transcription factor [ 137 ]. As OsERF87 and OsWRKY76 bind at the RSOsPR10 promoter, they antagonistically regulate RSOsPR10 expression.…”

Pinpointing genomic regions associated with root system architecture in rice through an integrative meta-analysis approach

“…OsWRKY31 found in MQTL6-3 can act in the auxin signal transduction pathway, and its ectopic expression reduced the length and formation of lateral roots (Zhang et al 2008). OsWRKY76 lying in MQTL9-3 was found to regulate cellular responses to abiotic and biotic types of stress (Yokotani et al 2013) partly by regulating the expression of RSOsPR10 in the lateral roots of rice, improving the mass and growth of root and increasing tolerance to high salt environments and soil desiccation (Yamamoto et al 2018).…”