Expression of hydroxytyrosol and oleuropein biosynthetic genes are correlated with metabolite accumulation during fruit development in olive, Olea europaea , cv. Koroneiki

Mougiou, Niki; Trikka, Fotini A.; Trantas, Emmanouil; Ververidis, Filippos; Makris, Antonios M.; Argiriou, Anagnostis; Vlachonasios, Konstantinos E.

doi:10.1016/j.plaphy.2018.05.004

Cited by 32 publications

(31 citation statements)

References 29 publications

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“…It is important to point out that very few genes related to the secoiridoid metabolism in olive have been functionally characterized. In most of the previous studies, putative genes have been selected based on sequence homologies, but further functional evaluation of the encoded proteins is required [42]. In this sense, the data obtained with OeAAS seem to be comparable to those reported by Alagna et al [43] for an olive iridoid synthase gene ( OeISY ), having an expression pattern highly correlated to secoiridoid accumulation that, together with the high specificity of the coded protein for 8-oxogeranial, suggest that OeISY is responsible for the synthesis of the iridoid scaffold in olive.…”

Section: Resultsmentioning

confidence: 99%

An Aromatic Aldehyde Synthase Controls the Synthesis of Hydroxytyrosol Derivatives Present in Virgin Olive Oil

et al. 2019

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The phenolic composition of virgin olive oil (VOO) is strongly determined by the content and distribution of secoiridoid phenolic glucosides present in the olive fruit. Among them, oleuropein is the most abundant in olive mesocarp and is characterized by containing an hydroxytyrosol residue in its chemical structure. Hydroxytyrosol-containing molecules are those that exhibit the most important biological activities in virgin olive oil. In this regard, we identified an aromatic aldehyde synthase gene (OeAAS) from an olive transcriptome, which was synthesized, expressed in Eschrichia coli, and purified its encoded protein. The recombinant OeAAS is a bifunctional enzyme catalyzing decarboxylation and amine-oxidation reactions in a single step. OeAAS displays strict substrate specificity for l-DOPA to form 2,4-dihydroxyphenylacetaldehyde, the immediate precursor of hydroxytyrosol. In addition to the biochemical characterization of the enzyme, the expression analysis carried out in different olive cultivars and ripening stages indicate that OeAAS gene is temporally regulated in a cultivar-dependent manner. High correlation coefficients were found between OeAAS expression levels and the phenolic content of olive fruits and oils, which supports a key role for OeAAS in the accumulation of hydroxytyrosol-derived secoiridoid compounds in olive fruit and virgin olive oil.

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Section: Resultsmentioning

confidence: 99%

An Aromatic Aldehyde Synthase Controls the Synthesis of Hydroxytyrosol Derivatives Present in Virgin Olive Oil

et al. 2019

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“…Among the effects of high temperatures on chemical parameters characterized in the current study, were total polyphenols and the fatty acid profile of olive oil. Polyphenol levels are known to decrease during fruit development [52,53]. These levels are often positively correlated with water stress but in some cases show a divergent trend [54,55].…”

Section: High Temperatures Environment Affects Oil Qualitymentioning

confidence: 99%

High temperature environment reduces olive oil yield and quality

et al. 2020

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Global warming is predicted to have a negative effect on plant growth due to the damaging effect of high temperatures. In order to address the effect of high temperature environments on olive oil yield and quality, we compared its effect on the fruit development of five olive cultivars placed in a region noted for its high summer temperatures, with trees of the same cultivars placed in a region of relatively mild summers. We found that the effects of a high temperature environment are genotype dependent and in general, high temperatures during fruit development affected three important traits: fruit weight, oil concentration and oil quality. None of the tested cultivars exhibited complete heat stress tolerance. Final dry fruit weight at harvest of the 'Barnea' cultivar was not affected by the high temperature environment, whereas the 'Koroneiki', 'Coratina', 'Souri' and 'Picholine' cultivars exhibited decreased dry fruit weight at harvest in response to higher temperatures by 0.2, 1, 0.4 and 0.2 g respectively. The pattern of final oil concentration was also cultivar dependent, 'Barnea', 'Coratina' and 'Picholine' not being affected by the high temperature environment, whereas the 'Koroneiki' and 'Souri' cultivars showed a decreased dry fruit oil concentration at harvest under the same conditions by 15 and 8% respectively. Regarding the quality of oil produced, the 'Souri' cultivar proved more tolerant to a high temperature environment than any other of the cultivars analyzed in this study. These results suggest that different olive cultivars have developed a variety of mechanisms in dealing with high temperatures. Elucidation of the mechanism of each of these responses may open the way to development of a variety of olives broadly adapted to conditions of high temperatures.

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“…So far, a large number of functional and regulatory genes implicated in abiotic and biotic stress responses have been identified in a diversity of plants. In particular, we characterized some functional or regulatory genes controlling metabolism of N, PAs, ethylene, and abscisic acid that are involved in various biological processes [22,33,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72].…”

Section: Major Genes Involved In Abiotic and Biotic Stress Responsesmentioning

confidence: 99%

The Interplay among Polyamines and Nitrogen in Plant Stress Responses

Paschalidis

Tsaniklidis

Wang

et al. 2019

Plants

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The interplay between polyamines (PAs) and nitrogen (N) is emerging as a key factor in plant response to abiotic and biotic stresses. The PA/N interplay in plants connects N metabolism, carbon (C) fixation, and secondary metabolism pathways. Glutamate, a pivotal N-containing molecule, is responsible for the biosynthesis of proline (Pro), arginine (Arg) and ornithine (Orn) and constitutes a main common pathway for PAs and C/N assimilation/incorporation implicated in various stresses. PAs and their derivatives are important signaling molecules, as they act largely by protecting and preserving the function/structure of cells in response to stresses. Use of different research approaches, such as generation of transgenic plants with modified intracellular N and PA homeostasis, has helped to elucidate a plethora of PA roles, underpinning their function as a major player in plant stress responses. In this context, a range of transgenic plants over-or under-expressing N/PA metabolic genes has been developed in an effort to decipher their implication in stress signaling. The current review describes how N and PAs regulate plant growth and facilitate crop acclimatization to adverse environments in an attempt to further elucidate the N-PAs interplay against abiotic and biotic stresses, as well as the mechanisms controlling N-PA genes/enzymes and metabolites.

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Expression of hydroxytyrosol and oleuropein biosynthetic genes are correlated with metabolite accumulation during fruit development in olive, Olea europaea , cv. Koroneiki

Cited by 32 publications

References 29 publications

An Aromatic Aldehyde Synthase Controls the Synthesis of Hydroxytyrosol Derivatives Present in Virgin Olive Oil

An Aromatic Aldehyde Synthase Controls the Synthesis of Hydroxytyrosol Derivatives Present in Virgin Olive Oil

High temperature environment reduces olive oil yield and quality

The Interplay among Polyamines and Nitrogen in Plant Stress Responses

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