Expression of human chorionic gonadotropin (hCG)/luteinizing hormone receptors and regulation of the cyclooxygenase-1 gene by exogenous hCG in human fetal membranes.

Tóth, Péter T.; Li, X; Lei, Zhenmin; Rao, Ch.V.

doi:10.1210/jcem.81.3.8772613

Cited by 20 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As it is expressed constitutively in many tissues [Langenbach et al., 1995; Simmons et al, 1989], and traditionally not believed to mediate stimulated biological events, Cox-1 has been considered a ''housekeeping'' gene [De Witt and Smith, 1988;Langenbach et al, 1995;Simmons et al, 1989]. Recently, however, the isoform has proven, in some circumstances, to be regulated [Samet et al, 1995;Smith et al, 1993Smith et al, , 1996Toth et al, 1996]. In endothelial cells, for example, cox-1 levels mirror the state of differentiation [Samet et al, 1995].…”

Section: Discussionmentioning

confidence: 99%

1,25 dihydroxyvitamin D3 and dexamethasone induce the cyclooxygenase 1 gene in osteoclast-supporting stromal cells

et al. 1999

View full text Add to dashboard Cite

Commitment of members of the monocyte/macrophage family to the bone resorptive phenotype, in vitro, requires contact, of these osteoclast precursors, with osteoblasts or related stromal cells. The osteoclast-inductive properties of these stromal cells are typically expressed, however, only in the presence of steroid hormones such as 1,25 dihydroxyvitamin D (1,25D3) and dexamethasone (DEX). To gain insight into the means by which steroid treated accessory cells induce osteoclast differentiation we asked, using differential RNA display (DRD), if gene expression by this stromal cell population differs from that of their untreated, non-osteoclastogenic counterpart. We identified four known genes specifically expressed by 1,25D3/DEX-treated ST2 stromal cells: 1) a family of rat organic anion transporters, 2) Na/K ATPase ss-subunit, 3) tazarotene-induced gene 2 (TIG2), and 4) prostaglandin G/H synthase I, or cyclooxygenase 1 (Cox-1). The regulation of these genes in 1,25D3/DEX-treated ST2 cells was demonstrated by Northern blot analysis of treated (osteoclast-supporting) and untreated (non-osteoclast-supporting) ST2 cells; the genes have a limited and specific tissue mRNA expression pattern. Northern blot analysis of treated and untreated ST2 cell total RNA using either a DRD-derived Cox-1 cDNA or a Cox-1 specific oligonucleotide confirmed the steroid regulation of Cox-1 mRNA. Surprisingly, there is no detectable expression by untreated or steroid exposed ST2 cells, of Cox-2, the classical regulated cyclooxygenase isoform. In contrast to 1, 25D3/DEX, serum treatment rapidly induces Cox-2 mRNA, substantiating the capacity of ST2 cells to express the gene. These data establish that steroid induction of the osteoclastogenic properties of stromal cells is attended by Cox gene expression, a phenomenon consistent with the capacity of eicosinoids to impact the resorptive process. The response of osteoclast-supporting ST2 cells to 1,25D3/DEX treatment may be one prostaglandin-mediated event which specifically involves Cox-1 regulation.

show abstract

Section: Discussionmentioning

confidence: 99%

1,25 dihydroxyvitamin D3 and dexamethasone induce the cyclooxygenase 1 gene in osteoclast-supporting stromal cells

et al. 1999

View full text Add to dashboard Cite

show abstract

“…The amniotic epithelium is not only one of the main sources of prostaglandins, especially prostaglandin E 2 (4), but also expresses prostaglandin-biosynthesis enzymes such as phospholipase, prostaglandin synthase, and cyclooxygenase (5). Moreover, these enzymes are regulated by human chorionic gonadotropin (hCG), the receptors of which are found on the amniotic epithelium (6).…”

Section: Basic Structure and Function Of Amniotic Membranementioning

confidence: 99%

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

et al. 2007

View full text Add to dashboard Cite

Abstract. Regenerative medicine is a new field based on the use of stem cells to generate biological substitutes and improve tissue functions, restoring damaged tissue with high proliferability and differentiability. It is of interest as a potential alternative to complicated tissue / organ transplantation. Recently, amnion-derived cells have been reported to have multipotent differentiation ability, and these cells have attracted attention as a cell source for cell-transplantation therapy. The amnion possesses considerable advantageous characteristics: the isolated cells can differentiate into all three germ layers; they have low immunogenicity and anti-inflammatory functions; and they do not require the sacrifice of human embryos for their isolation, thus avoiding the current controversies associated with the use of human embryonic stem cells. Moreover, we developed human amniotic cell-sheets using a novel culture surface coated with a noncytotoxic, temperature-responsive elastic protein-based polymer. We also generated a "hyperdry-amnion", which has already been applied clinically in the ophthalmological field. Compared to cryopreserved fresh amnion, "hyper-dry-amnion" is easy to handle and has started to bring good results to patients. These materials from the amnion are also expected to open a new field in tissue engineering. Thus, amnion, which had been discarded after parturition, has started to be appreciated as an attractive material in the field of regenerative medicine. In this review, the most recent and relevant clinical and experimental data about the use of amniotic membrane and cells derived from it are described.

show abstract

“…9). COX-1 is constitutively expressed in most cell types and has been considered a housekeeping gene (10), although more recent evidence shows regulated expression in both the inflammatory and reproductive systems (11,12). COX-2 is a dynamic isoform induced by specific stimuli in specific cell types (13).…”

mentioning

confidence: 99%

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

Bracken¹

1997

Endocrinology

View full text Add to dashboard Cite

Prostaglandin F 2␣ (PGF 2␣ ) secretion is lowest at midcycle and highest on day 15 at luteolysis in the cycling guinea pig uterus and is inversely related to serum progesterone levels. An increase in 17-␤ estradiol (E 2 ) occurs only towards the end of the cycle. To investigate the effect of steroids on the control of uterine PGF 2␣ metabolism at the level of gene expression we established a primary cell culture model of day 15 cycling guinea pig endometrial cells. We cloned guinea pig cDNAs for cyclooxygenase 2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (PGDH) that converts PGF 2␣ to biologically inactive 13,14-dihydro-15-keto PGF 2␣ (PGFM) and a fragment of cyclooxygenase-1 (COX-1). They were found to bear 87% and 90% homology at the amino acid level to their human counterparts for COX-2 and PGDH, respectively, retaining all functional sites. Purified epithelial and stromal cell subcultures were primed with medium containing either E 2 or medroxyprogesterone acetate (MPA) for 24 h. They were then treated for a further 4 or 24 h either withdrawing the steroid, maintaining the priming steroid, or supplementing with both steroids, before harvesting conditioned media and RNA. Epithelial cells secreted 30-fold more PGF 2␣ compared with stromal cells (e.g. 7.8 Ϯ 0.7 vs. 0.26 Ϯ 0.09 pg/ng DNA•24 h), and PGF 2␣ secretion levels were approximately 15-fold higher than those of PGFM (e.g. 7.8 Ϯ 0.7 vs. 0.45 Ϯ 0.16 pg/ng DNA⅐24 h, for epithelial cells). COX-1 transcripts were low and unaffected by treatment in both cell types. COX-2 transcripts were more abundant in epithelial than stromal cells. Steroid-modulated, COX-2 dependent changes in PGF 2␣ secretion were observed. The addition of MPA to E 2 primed cells caused a decrease in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 h. Conversely, the addition of E 2 to MPA primed epithelial cells led to an increase in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 and 24 h. The withdrawal of E 2 caused a fall in PGF 2␣ secretion and COX-2 transcripts after 24 h. In contrast, PGDH transcripts were more abundant in stromal than epithelial cells and were up-regulated by the addition of MPA to E 2 primed cells. These in vitro observations are in keeping with the secretory profile seen in vivo in the cycling guinea pig uterus suggesting that 1) the fall of E 2 and the coinciding rise in progesterone seen in the early cycle lead to a reduction in PGF 2␣ levels; and 2) the rise of E 2 in the late cycle on a progesterone primed uterus is the stimulus for an increase in uterine PGF 2␣ production. Our findings suggest a differential role for uterine stroma and epithelium in vivo whereby the former acts to remove (via PGDH), and the latter to produce (via COX-2) biologically active prostaglandin. (Endocrinology 138: [237][238][239][240][241][242][243][244][245][246][247] 1997)

show abstract

Expression of human chorionic gonadotropin (hCG)/luteinizing hormone receptors and regulation of the cyclooxygenase-1 gene by exogenous hCG in human fetal membranes.

Cited by 20 publications

References 0 publications

1,25 dihydroxyvitamin D3 and dexamethasone induce the cyclooxygenase 1 gene in osteoclast-supporting stromal cells

1,25 dihydroxyvitamin D3 and dexamethasone induce the cyclooxygenase 1 gene in osteoclast-supporting stromal cells

The Potential of Amniotic Membrane/Amnion-Derived Cells for Regeneration of Various Tissues

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

Contact Info

Product

Resources

About