Expression of host resistance to Salmonella typhi and Salmonella typhimurium: bacterial survival within macrophages of murine and human origin

Vladoianu, I. R.; Chang, Huilan; Péchère, Jean-Claude

doi:10.1016/0882-4010(90)90072-x

Cited by 63 publications

(44 citation statements)

References 19 publications

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“…This may allow gentamicin to enter the macrophage and kill intracellular bacteria, and therefore the difference in recovery of S. typhimurium and S. dublin compared to S. choleraesuis is probably greater than that measured by viable counts. These persistence data appear to be in contrast to those obtained with murine macrophages, from which it was concluded that the persistence of S. typhimurium and S. typhi directly correlated with their host specificity (Lissner et al, 1985 ;Vladoianu et al, 1990 ;Alpuche-Aranda et al, 1995 ;Ishibashi & Arai, 1996 ;Schwan & Kopecko, 1997). However, S. typhimurium can persist relatively well not only in murine macrophages, but also in human-monocyte-derived macrophages, chicken peritoneal exudate cells and porcine alveolar macrophages (Vladoianu et al, 1990 ;Alpuche-Aranda et al, 1995 ;Ishibashi & Arai, 1996 ; M. S. Chadfield & J. E. Olsen, personal communication ; this study).…”

Section: Discussioncontrasting

confidence: 43%

“…However, porcine alveolar macrophages may differ in some of their properties to tissue macrophages in other systemic organs, and therefore may not necessarily accurately model the interaction of Salmonella with macrophages in the porcine liver and spleen. Macrophage-derived cell lines were not used because they are dramatically more permissive for uptake and\or intracellular replication of Salmonella serotypes compared to primary macrophages (Buchmeier & Heffron, 1989 ;Vladoianu et al, 1990) and would appear to be inherently unsuitable for such studies. For example, in murine macrophage-like cells, some studies find no difference between S. typhimurium and S. typhi (Vladoianu et al, 1990 ;Hirose et al, 1997), and others report differences in either uptake, but not persistence (Pascopella et al, 1995), or persistence, but not uptake (Schwan & Kopecko, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Macrophage-derived cell lines were not used because they are dramatically more permissive for uptake and\or intracellular replication of Salmonella serotypes compared to primary macrophages (Buchmeier & Heffron, 1989 ;Vladoianu et al, 1990) and would appear to be inherently unsuitable for such studies. For example, in murine macrophage-like cells, some studies find no difference between S. typhimurium and S. typhi (Vladoianu et al, 1990 ;Hirose et al, 1997), and others report differences in either uptake, but not persistence (Pascopella et al, 1995), or persistence, but not uptake (Schwan & Kopecko, 1997). In the present study, the majority of macrophage experiments were performed using non-opsonized bacteria, since preliminary experiments showed that opsonization was not a prerequisite for efficient phagocytosis and did not influence subsequent persistence, as has sometimes been reported for other serotype-host combinations (Ishibashi & Arai, 1996).…”

Section: Discussionmentioning

confidence: 99%

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Interaction of Salmonella serotypes with porcine macrophages in vitro does not correlate with virulence

et al. 2000

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The interaction between Salmonella serotypes and macrophages is potentially instrumental in determining the outcome of infection. The nature of this interaction was characterized with respect to virulence and serotype-host specificity using pigs as the infection model. Experimental infection with Salmonella typhimurium , Salmonella choleraesuis or Salmonella dublin resulted in enteric, systemic or asymptomatic infection, respectively, which correlates well with the association of S. choleraesuis with systemic disease in pigs in epidemiological studies. Persistence within porcine alveolar macrophages in vitro did not directly correlate with virulence since S. typhimurium persisted in the highest numbers, and S. choleraesuis in the lowest. Comparison to other studies revealed that the relatively high persistence of S. typhimurium in macrophages correlates with its virulence in a broad range of animals : this could be a virulence mechanism for broad-hostrange serotypes. There were little or no significant differences in the induction of pro-inflammatory cytokines by macrophages infected with the three serotypes. S. typhimurium and S. dublin, but not S. choleraesuis, damaged porcine macrophages, and the mechanism of damage did not resemble apoptosis. In conclusion, the virulence of Salmonella serotypes in pigs did not directly correlate with their interaction with porcine macrophages in vitro. The interaction of Salmonella and macrophages in vitro may not accurately model their interaction in vivo, and this will form the basis of further study.

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Section: Discussioncontrasting

confidence: 43%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Interaction of Salmonella serotypes with porcine macrophages in vitro does not correlate with virulence

et al. 2000

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“…Several lines of evidence suggest this hypothesis for Typhi human-restriction. Vladoianu and colleagues [200] demonstrated that Typhi strains persisted in human monocyte-derived macrophages, but not in murine macrophages, confirming that the macrophage control over salmonella infection could influence host specificity. On the other hand, Typhimurium, which causes typhoid in mice and diffuse enteritis and, less frequently, bacteraemia in humans, is able to survive in mouse macrophages and in human macrophages [200].…”

Section: Extraintestinal Infection : Interaction With the Reticulo-enmentioning

confidence: 96%

“…Vladoianu and colleagues [200] demonstrated that Typhi strains persisted in human monocyte-derived macrophages, but not in murine macrophages, confirming that the macrophage control over salmonella infection could influence host specificity. On the other hand, Typhimurium, which causes typhoid in mice and diffuse enteritis and, less frequently, bacteraemia in humans, is able to survive in mouse macrophages and in human macrophages [200]. Furthermore, Typhi induces ruffling and micropinocitosis in murine macrophages, but internalization is followed by formation of fewer numbers of SP than Typhimurium, an indicator of reduced capability to survive within macrophages that is essential in pathogenesis [201,202].…”

Section: Extraintestinal Infection : Interaction With the Reticulo-enmentioning

confidence: 96%

Host adapted serotypes of Salmonella enterica

et al. 2000

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Salmonella constitutes a genus of zoonotic bacteria of worldwide economic and health importance. The current view of salmonella taxonomy assigns the members of this genus to two species: S. enterica and S. bongori. S. enterica itself is divided into six subspecies, enterica, salamae, arizonae, diarizonae, indica, and houtenae, also known as subspecies I, II, IIIa, IIIb, IV, and VI, respectively [1]. Members of Salmonella enterica subspecies enterica are mainly associated with warm-blooded vertebrates and are usually transmitted by ingestion of food or water contaminated by infected faeces. The pathogenicity of most of the distinct serotypes remains undefined, and even within the most common serotypes, many questions remain to be answered regarding the interactions between the organism and the infected host.Salmonellosis manifests itself in three major forms: enteritis, septicaemia, and abortion, each of which may be present singly or in combination, depending on both the serotype and the host involved. Although currently over 2300 serovars of Salmonella are recognized, only about 50 serotypes are isolated in any significant numbers as human or animal pathogens [2, 3] and they all belong to subspecies enterica. Of these, most cause acute gastroenteritis characterized by a short incubation period and a severe systemic disease in man or animals, characterized by septicaemia, fever and/or abortion, and such serotypes are often associated with one or few host species [4–6].It is the intention of this review to present a summary of current knowledge of these host-adapted serotypes of S. enterica. The taxonomic relationships between the serotypes will be discussed together with a comparison of the pathology and pathogenesis of the disease that they cause in their natural host(s). Since much of our knowledge on salmonellosis is based on the results of work on Typhimurium, this serotype will often be used as the baseline in discussion. It is hoped that an appreciation of the differences that exist in the way these serotypes interact with the host will lead to a greater understanding of the complex host–parasite relationship that characterizes salmonella infections.

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