Expression of high affinity iron uptake systems by clinical isolates of Klebsiella

Williams, Phyllis H.

doi:10.1016/0378-1097(87)90356-9

Cited by 10 publications

(10 citation statements)

References 8 publications

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“…Moreover, because enterobactin is produced by important pathogens, such as E. coli (35) and especially K. pneumoniae (43), which often colonize the same sites as P. aeruginosa (e.g., respiratory tract, urinary tract, and burns) (4,29,31), there is the chance that enterobactin may be present at the site of a P. aeruginosa infection, where it may stimulate growth and, ultimately, assist pathogenesis. Furthermore, although its effectiveness as a supplier of iron appears diminished in serum (25), enterobactin may well be more effective in the locales where serum proteins should be reduced.…”

Section: Discussionmentioning

confidence: 99%

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Enterobactin-mediated iron transport in Pseudomonas aeruginosa

1990

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A pyoverdine-deficient strain of Pseudomonas aeruginosa was unable to grow in an iron-deficient minimal medium in the presence of the nonmetabolizable iron chelator ethylene diamine-di(omega-hydroxyphenol acetic acid) (EDDHA), although addition of enterobactin to EDDHA-containing minimal media did restore growth of the pyoverdine-deficient P. aeruginosa. Consistent with the apparent ability of enterobactin to provide iron to P. aeruginosa, enterobactin-dependent 55Fe3+ uptake was observed in cells of P. aeruginosa previously grown in an iron-deficient medium containing enterobactin (or enterobactin-containing Escherichia coli culture supernatant). This uptake was energy dependent, was observable at low concentrations (60 nM) of FeCl3, and was absent in cells cultured without enterobactin. A novel protein with a molecular weight of approximately 80,000 was identified in the outer membranes of cells grown in iron-deficient minimal medium containing enterobactin, concomitant with the induction of enterobactin-dependent iron uptake. A Tn501 insertion mutant lacking this protein was isolated and shown to be deficient in enterobactin-mediated iron transport at 60 nM FeCl3, although it still exhibited enterobactin-dependent growth in iron-deficient medium containing EDDHA. It was subsequently observed that the mutant was, however, capable of enterobactin-mediated iron transport at much higher concentrations (600 nM) of FeCl3. Indeed, enterobactin-dependent iron uptake at this concentration of iron was observed in both the mutant and parent strains irrespective of whether they had been cultured in the presence of enterobactin.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

“…[33], Klebsiella spp. [43], and Escherichia coli [41]), for example, synthesize both enterobactin (enterochelin) and aerobactin and can transport iron complexed to either of these siderophores. E. coli, Salmonella typhimurium, and Klebsiella pneumoniae are also capable of utilizing siderophores produced by other microorganisms.…”

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confidence: 99%

Enterobactin-mediated iron transport in Pseudomonas aeruginosa

1990

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“…Outer membrane proteins were separated on 11.5% sodium dodecyl sulfate (SDS)-polyacrylamide gels, transferred by electroblotting onto nitrocellulose membranes, and probed with a polyclonal rabbit antibody raised against the purified 81-kDa FepA protein of E. coli (53). Blots were developed with protein A-alkaline phosphatase (Calbiochem) and visualized with the BCIP/NBT liquid substrate system (Sigma).…”

Section: Synthesis Of Ahls and Cyclic Dipeptides N-(3-oxododecanoyl)mentioning

confidence: 99%

“…However, the data provided here provide an alternative explanation. Almost all of the organisms shown to respond to NE or the E. coli AI are known to be capable of making and/or transporting ferric enterobactin or related catechol siderophores (6,8,10,18,35,47,53,54). While gram-positive bacteria such as Listeria monocytogenes do not produce enterobactin, some are capable of using enterobactin and NE as exogenous siderophores (6).…”

Section: Figmentioning

confidence: 99%

The Growth Response of Escherichia coli to Neurotransmitters and Related Catecholamine Drugs Requires a Functional Enterobactin Biosynthesis and Uptake System

et al. 2002

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The neurotransmitter norepinephrine (NE) stimulates the growth of low inocula of Escherichia coli in a minimal medium (SAPI) supplemented with serum (SAPI؉serum) and induces the production of an "autoinducer" (AI) which, in turn, promotes E. coli growth in the absence of NE. Given the importance of NE, epinephrine, and their corresponding adrenergic agonists and antagonists in clinical medicine, we sought to investigate the molecular basis for these observations. Using a variety of NE precursors, metabolites, and therapeutic agents, we demonstrated that their ability to stimulate E. coli growth in SAPI؉serum is dependent on the presence of a catechol (1,2-dihydroxybenzene) moiety with maximal activity requiring a two-carbon substituent incorporating a terminal primary amine. Serum contains the iron-binding glycoprotein, transferrin, and when SAPI؉serum was supplemented with sufficient Fe 3؉ to saturate transferrin, growth inhibition was relieved. Other metal cations, including Mg 2؉ , Ca 2؉ , and Zn 2؉ , had no effect. These data suggested that the stimulation of E. coli growth by NE in SAPI؉serum may involve the catecholate siderophore, enterobactin, a cyclic triester of 2,3-dihydroxybenzoylserine. Consistent with this hypothesis, E. coli strains with mutations in ferrienterobactin transport (fepA or tonB) or enterobactin biosynthesis (entA) did not respond to NE. Furthermore, NE induced expression of the ferrienterobactin receptor, FepA, during growth in SAPI؉serum. The enterobactin degradation product, 2,3-dihydroxybenzoylserine (DBS) was as effective as NE in stimulating the growth of E. coli and mutations in fepA or tonB abolished the DBS-dependent growth stimulation. In contrast to NE, however, DBS stimulated the growth of the entA mutant. Moreover, after growth in an ironlimited M9 medium in the absence of NE, ethyl acetate extracts of the E. coli entA ؉ parent but not of the entA mutant contained AI, i.e., stimulated the growth of E. coli in SAPI؉serum. Taken together, these data show that when low numbers of E. coli are inoculated into SAPI؉serum, NE, DBS, and related catecholamines induce the enterobactin iron uptake system. This, in turn, facilitates iron sequestration from transferrin and indicates that the AI present in NE-conditioned SAPI؉serum medium is enterobactin and its DBS breakdown products. Bacterial cells undergo a wide variety of physiological and morphological adaptations in response to chemical and physical changes in their environment. From the prokaryotic perspective, the successful interaction of bacterial cells with mammalian host tissues depends not only on a coordinated response to environmental cues such as nutrient availability, population density, temperature, osmolarity and pH (37, 56) but also on diverse host cell effector molecules. In general, the influence of host signaling molecules on bacteria has received relatively little attention (25). However, hormones such as epinephrine (12) and insulin (57), the neurotransmitter norepinephrine (NE [34]), and cytokines such as int...

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“…Enterochelin is synthesised by Salmonella, Klebsiella and Shigella species [19,130,133,[161][162][163], while the aerobactin system is widely distributed among strains of Enterobacter, Salmonella, Klebsiella, Shigella, Citrobacter, Proteus, Morganella, Yersinia, Serratia and Hafnia [162,[164][165][166]. Some members of the Enterobacteriaceae, such as Erwinia, Pantoea, Enterobacter and Hafnia spp., synthesise ferrioxamine-type siderophores [167,168] while a-keto acids and c~-hydroxycarboxylic acids produced by enzymic amino acid deamination have been shown to have siderophore activity and to support the growth of Proteus, Prot~idencia and Morganella spp.…”

Section: Siderophore Systems In Other Bacteriamentioning

confidence: 99%

Iron uptake mechanisms of pathogenic bacteria

Wooldridge

1993

FEMS Microbiology Reviews

105

127

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Most of the iron in a mammalian body is complexed with various proteins. Moreover, in response to infection, iron availability is reduced in both extracellular and intracellular compartments. Bacteria need iron for growth and successful bacterial pathogens have therefore evolved to compete successfully for iron in the highly iron-stressed environment of the host's tissues and body fluids. Several strategies have been identified among pathogenic bacteria, including reduction of ferric to ferrous iron, occupation of intracellular niches, utilisation of host iron compounds, and production of siderophores. While direct evidence that high affinity mechanisms for iron acquisition function as bacterial virulence determinants has been provided in only a small number of cases, it is likely that many if not all such systems play a central role in the pathogenesis of infection.

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Expression of high affinity iron uptake systems by clinical isolates of Klebsiella

Cited by 10 publications

References 8 publications

Enterobactin-mediated iron transport in Pseudomonas aeruginosa

Enterobactin-mediated iron transport in Pseudomonas aeruginosa

The Growth Response of Escherichia coli to Neurotransmitters and Related Catecholamine Drugs Requires a Functional Enterobactin Biosynthesis and Uptake System

Iron uptake mechanisms of pathogenic bacteria

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