Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia

Джалилова, Д. Ш.; Diatroptov, M. E.; Цветков, И.С.; Makarova, Olga; Кузнецов, С. Л.

doi:10.1007/s10517-018-4264-x

Cited by 26 publications

(21 citation statements)

References 13 publications

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“…It has been demonstrated that in rats susceptible to hypoxia, under normal conditions, the level of HIF-1α in the neocortex is 1.7 times higher than in tolerant rats 27. Earlier, we demonstrated that at different periods after acute hypoxic exposure, rats tolerant and susceptible to hypoxia are characterized by the variability in expression of Nf-kb and TGF-β cytokine content, modulating inflammatory responses 32. This may cause the distinctive features of the development of inflammation.…”

Section: Introductionmentioning

confidence: 71%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

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The aim of the study was to characterize the severity of the systemic inflammatory response induced by lipopolysaccharide (LPS) in animals with different resistance levels to hypoxia. Materials and methods: Two to three months old male Wistar rats (220-240 g) were divided according to hypoxia tolerance in a hypobaric chamber. After a month, they were injected intraperitoneally with Escherichia coli LPS at a dose of 1.5 mg/kg. After 3, 6 and 24 hours of LPS injection, we studied the levels of IL-1β, C-reactive protein (CRP) and TGF-β in the serum, the expression of Hif-1α and Nf-kb in the liver, morphological disorders in the lung and ex vivo production of IL-10 by splenic cells activated by ConA. Results: In the early periods after the injection of LPS, increase in Nf-kb expression in the liver was observed only in the rats susceptible to hypoxia. After 6 hours of LPS injection, the number of neutrophils in the interalveolar septa of the lungs of rats susceptible to hypoxia was higher than in tolerant rats. This points to the development of more pronounced LPS-induced inflammation in the rats susceptible to hypoxia and is accompanied by increased expression of Hif-1α in the liver after 6 hours of LPS administration, serum IL-1β level after 3 hours and CRP level after 24 hours. The production of the anti-inflammatory cytokine IL-10 by the spleen was significantly decreased after 6 hours of LPS injection only in the animals tolerant to hypoxia. After 24 hours of LPS injection, a significant decrease in serum TGF-β level occurred in the rats tolerant to hypoxia in comparison with the control group, which improved the survival rates of the animals. Conclusion: We have demonstrated the differences in the severity of the LPS-induced inflammatory response in male Wistar rats with different resistance levels to hypoxia. Rats susceptible to hypoxia are characterized by a more pronounced inflammatory response induced by LPS.

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Section: Introductionmentioning

confidence: 71%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

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“…Usually for experimental animals, extreme altitudes, corresponding to respiratory disturbances and signs of asphyxia, are used. For outbred rats and Wistar rats, 11000–11500 m altitudes are used; for Sprague–Dawley rats, 9250–10668 m [ 9 , 11 , 12 , 14 , 18 , 19 , 126 , 192 , 193 , 194 ]. Another method for determining individual tolerance to hypoxia was proposed: a stepwise “ascent” of animals in a decompression chamber to platforms, corresponding to different altitudes until the recording of agonal breathing [ 195 ].…”

Section: Physiological Biochemical and Molecular-biological Charmentioning

confidence: 99%

“…The ratio may vary, depending on many factors (season, time of day, etc.). As practice shows, a significant ratio is accounted for normal animals (40–58%), the ratio of tolerant animals varies from 20 to 42%, and susceptible animals from 18 to 40% [ 9 , 11 , 12 , 126 , 194 ].…”

Section: Physiological Biochemical and Molecular-biological Charmentioning

confidence: 99%

“…In the heart of tolerant-to-hypoxia Sprague–Dawley rats, immediately after three consecutive measurements of hypoxia tolerance, the level of HIF-1α protein synthesis increased two times, while in susceptible rats the increase was not so significant [ 11 ]. We also showed an increase in the level of HIF-1 expression in tolerant-to-hypoxia Wistar rats after a single measurement of the resistance to oxygen deficiency, accompanied by an increase in VEGF and erythropoietin content [ 194 ]. An increase in the level of HIF-1 expression after hypoxic exposure in tolerant animals, apparently, contributes to their rapid and more effective acute adaptation to oxygen deficiency [ 208 , 210 , 223 ].…”

Section: Physiological Biochemical and Molecular-biological Charmentioning

confidence: 99%

“…A low level of carbonylated proteins in blood plasma after three exposures to hypoxia at an extreme altitude with an interval of a week has been performed to be used as a marker of tolerance to hypoxia [ 127 ]. We also demonstrated that, 90 min after a single hypoxic exposure at extreme altitude, the level of the oxidative stress marker 8-isoprostane increased in the blood serum only in susceptible-to-hypoxia rats [ 194 ].…”

Section: Physiological Biochemical and Molecular-biological Charmentioning

confidence: 99%

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Differences in Tolerance to Hypoxia: Physiological, Biochemical, and Molecular-Biological Characteristics

Джалилова

Makarova

2020

Biomedicines

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Hypoxia plays an important role in the development of many infectious, inflammatory, and tumor diseases. The predisposition to such disorders is mostly provided by differences in basic tolerance to oxygen deficiency, which we discuss in this review. Except the direct exposure of different-severity hypoxia in decompression chambers or in highland conditions, there are no alternative methods for determining organism tolerance. Due to the variability of the detection methods, differences in many parameters between tolerant and susceptible organisms are still not well-characterized, but some of them can serve as biomarkers of susceptibility to hypoxia. At the moment, several potential biomarkers in conditions after hypoxic exposure have been identified both in experimental animals and humans. The main potential biomarkers are Hypoxia-Inducible Factor (HIF)-1, Heat-Shock Protein 70 (HSP70), and NO. Due to the different mechanisms of various high-altitude diseases, biomarkers may not be highly specific and universal. Therefore, it is extremely important to conduct research on hypoxia susceptibility biomarkers. Moreover, it is important to develop a method for the evaluation of organisms’ basic hypoxia tolerance without the necessity of any oxygen deficiency exposure. This can contribute to new personalized medicine approaches’ development for diagnostics and the treatment of inflammatory and tumor diseases, taking into account hypoxia tolerance differences.

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Specific Behavioral Patterns Enhancing Hypoxia Resistance in Wistar Rats Subjected to Moderate Swimming Exercise

Kondashevskaya

2020

Bull Exp Biol Med

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Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia

Cited by 26 publications

References 13 publications

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Differences in Tolerance to Hypoxia: Physiological, Biochemical, and Molecular-Biological Characteristics

Specific Behavioral Patterns Enhancing Hypoxia Resistance in Wistar Rats Subjected to Moderate Swimming Exercise

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