Expression of HIF-1α, ANXA3, CD133 and their associations with clinicopathological parameters in human colon carcinoma

Chai, Xiaoyan; Xiao, Wei; Ren, Jiahui; Du, Kunli; Wu, Xingye; Feng, Fan; Zheng, Jun

doi:10.21037/tcr-22-1277

Cited by 3 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total score of 0-9 was obtained by multiplying the percentage of stained cells by the staining intensity. A total score of 0 was taken as negative, 1 and 2 as weak, 3 to 6 as moderate, and 7 to 9 as strongly positive [ 15 ]. Negative staining and weak positives (total score ≤2) were taken as low expressions (Figures 1 - 2 ).…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical Expression of the Stem Cell Marker CD133 in Colorectal Carcinoma

Suman¹,

Hota²,

Misra³

et al. 2023

Cureus

View full text Add to dashboard Cite

Background Colorectal carcinoma (CRC) is the second-leading cause of cancer-related death. Despite the combined (surgery, chemotherapy, radiotherapy, and immunotherapy) modalities of treatment, the prognosis remains poor, mostly because of recurrence and distant metastasis. Cancer stem cells (CSC) are thought to be responsible for the development and spread of tumors. Hence, targeted therapy against these cells hopes to reduce the chance of recurrence and metastasis and improve the prognosis. Many immune markers have been identified to detect CSC in CRC. Here, we tried to assess the immunohistochemical expression of the stem cell marker CD133 in colorectal carcinoma and its correlation with various pathological parameters. Methodology A total of 51 cases of CRC were analyzed. Immunohistochemistry for CD133 was done after standardization in our laboratory. Expression status was decided based on the total score obtained by multiplying the intensity score by the percentage score. CD133 expression was correlated with the age and gender of the patient, tumor location, histological grade, extent of invasion, lymphovascular invasion (LVI), perineural invasion (PNI), and nodal status. Results High CD133 expression was seen in 21 (41.17%) cases. There was no significant association between CD133 expression and the pathological parameters except the tumor site. CD133 expression was significantly higher as we moved from the proximal colon to the rectum. Conclusions CD133 expression was significantly higher in the distal part of the large intestine as compared to the proximal part. But there was no linear correlation between CD133 expression and histological grade, extent of invasion, or nodal status.

show abstract

Section: Methodsmentioning

confidence: 99%

Immunohistochemical Expression of the Stem Cell Marker CD133 in Colorectal Carcinoma

Suman¹,

Hota²,

Misra³

et al. 2023

Cureus

View full text Add to dashboard Cite

show abstract

“…HIF-1α is a nuclear protein that still has transcriptional activity under hypoxia. It will be overexpressed when the tumor cells proliferate rapidly [7]. Normally, HIF-1α experienced rapid degradation mediated by proteasome.…”

Section: Introductionmentioning

confidence: 99%

Expression of Trx-1, HIF-1α and their associations with clinicopathological parameters in gastric cancer

Niu

2023

Preprint

View full text Add to dashboard Cite

Background Although it is only a small redox protein, Thioredoxin-1 (Trx-1) can have a significant impact in many biological processes. In recent years, studies on the increased Trx-1 expression in different solid tumors have gradually increased, but its clinical and diagnostic significance in gastric cancer (GC) have not been confirmed. HIF-1α (Hypoxia-inducible factor-1α) plays an active role in many tumors’ metabolism, while the relevance between its expression levels and clinical value in GC remains to be investigated. Methods: Western blot (WB) and quantitative PCR (qPCR) were used to detect HIF-1α and Trx-1 mRNA and protein level in 20 freshly frozen GC and corresponding normal-tissues. In addition, the protein level of Trx-1 and HIF-1α in 162 GC were determined by immunohistochemistry (IHC) test. The relevance between HIF-1α and Trx-1 expression and the clinicopathological characteristics in GC was assessed as well. And Cox proportional risk model was applied to determine independent prognostic indicators. Results: When HIF-1α level increased, the Trx-1 mRNA and protein levels increased obviously in GC tissues, and they both were related to a bad prognosis (P < 0.001). There was an obvious positive relevance (r = 0.578, P = 0.000) between Trx-1 and HIF-1α expressions. Through multivariate analysis, for GC prognosis, Borrmann type, Trx-1 and HIF-1α were independent predictors (P < 0.001). Conclusion For GC patients, both Trx-1 and HIF-1α may be prospective prognostic indicators and therapeutic target.

show abstract

Relationship between gut microbiota and prognosis, clinical characteristics of colorectal cancer based on 16S rRNA sequencing

Huang

Wang

Yuan

et al. 2022

Preprint

View full text Add to dashboard Cite

Purpose Colorectal cancer is a high incidence tumor, immunotherapy has become a hot topic of research in recent years, at the same time, the dysregulation of intestinal microbiota is also gaining attention because it can lead to changes in body immunity. So, we want to explore the relationship between gut microbiota and colorectal cancer. Methods We performed 16S rRNA high-throughput sequencing of tumors and paraneoplastic tissues from 30 patients, followed by at least 5 years of follow-up. Microbiome analysis, survival analysis, and then immunohistochemistry and its correlation with microbiota were performed in 19 of these patients. Results OTU13530 and OTU17086, both representing g_Sediminibacterium, showed significant differences in normalized abundance in tumor and paraneoplastic tissues (FDR < 0.05 and log2FC > 1). Both of them correlated with T-stage (p = 0.016, p = 0.024) and Her-2 expression (p = 0.034, p = 0.001). The former correlated with HIF expression (p = 0.017), and the latter with EGFR expression (p = 0.003). OTU3504, representing o_Myxococcales, had a correlation with patient survival (p = 0.015, HR = 28.5(1.9–425.0)), its abundance correlated with tumor neural invasion (p = 0.005) and vascular invasion (p = 0.005). Conclusion There is no statistical difference in the diversity and homogeneity of the microbiota between tumor and paraneoplastic tissues. But the abundance of some specific microbiota differed significantly between tumor and paraneoplastic tissues, and these microbiota correlated with patient survival, Her-2, HIF and other proteins expression, T-stage, neurovascular invasion and other clinical traits.

show abstract

Expression of HIF-1α, ANXA3, CD133 and their associations with clinicopathological parameters in human colon carcinoma

Cited by 3 publications

References 41 publications

Immunohistochemical Expression of the Stem Cell Marker CD133 in Colorectal Carcinoma

Immunohistochemical Expression of the Stem Cell Marker CD133 in Colorectal Carcinoma

Expression of Trx-1, HIF-1α and their associations with clinicopathological parameters in gastric cancer

Relationship between gut microbiota and prognosis, clinical characteristics of colorectal cancer based on 16S rRNA sequencing

Contact Info

Product

Resources

About