Expression of HGF/c-Met Is Dynamically Regulated in the Dorsal Root Ganglions and Spinal Cord of Adult Rats following Sciatic Nerve Ligation

Abstract

HGF/c-Met system is spatiotemporally regulated by a unique pattern of signaling pathways induced by peripheral nerve injury, and these pathways have a role in promoting the survival of injured neurons, especially adult DRG sensory neurons.

Slit–Robo GTPase‐Activating Proteins are Differentially Expressed in Murine Dorsal Root Ganglia: Modulation by Peripheral Nerve Injury

“…The following pairs of double immunofluorescence were carried out: (1) srGAP1 and neurofilament 200, (2) srGAP3 and S100, (3) srGAP3 and glial fibrillary acidic protein (GFAP), (4) srGAP3 and NF200, and (5) srGAP3 and Robo2. Final dilutions of these primary antibodies were: mouse anti‐srGAP1 (1:300) (Abcam, Hong Kong), rabbit anti‐srGAP3 (1:400) (Abcam, Hong Kong), rat anti‐srGAP3 (Abcam, Hong Kong), mouse anti‐GFAP (1:400) (Millipore USA), chicken anti‐GFAP (1:400) (Millipore), rat anti‐NF200 (1:400; Sigma), mouse anti‐Robo2 (1:400) (Abcam, Hong Kong), rabbit anti‐srGAP3 (1:400), and mouse anti‐S100 (1:400) (Millipore) antibodies (Zheng et al,2008,2010). Secondary antibodies included fluorescein isothiocyanate (FITC)‐goat anti‐mouse IgG (1:200), FITC‐goat anti‐chicken IgG (1:200), Cy3‐goat anti‐rabbit IgG (1:200) (Boster, China), FITC‐goat anti‐rat IgG (1:200) (Boster, China).…”

In vivo targeted peripheral nerve imaging with a nerve-specific nanoscale magnetic resonance probe

“…All of these changes induced Wallerian degeneration, leading to the lysis and elimination of the distal part of the axons and myelin debris; however, the proximal part of the myelin from the injury site remained intact. During the regeneration after PNI, the regenerated myelin in the distal part of injury site was re-assembled with P0 and PMP22 [33][34][35]42,43]. Therefore, P0 and PMP22 kDa are peripheral nerve-specific proteins that have a differential expression after PNI.…”

“…After PNI, the injury signal induced a series of molecular and cellular changes at the injury site [33][34][35]. In our previous study, we found that there are different pathological/ molecular changes in the proximal and distal sites after PNI.…”

“…In our previous study, we found that there are different pathological/ molecular changes in the proximal and distal sites after PNI. These specific proteins can serve as an ideal target for designing special imaging probes [33,34]. Additionally, an appropriate signal-tonoise ratio (SNR) and resolution image was crucial to a molecular MRI strategy.…”

Up-regulation of Robo1 in dorsal root ganglia after sciatic nerve transection in rats

“…Following additional rinses, sections were mounted on gelatin subbed slides, air dried, dehydrated in ethanol, cleared in xylene, and coverslipped with DPX (Fluka Chemie AG, Switzerland). As a negative control, sections were incubated without primary antibody and processed as described above (Zheng et al, 2008(Zheng et al, , 2010.…”