Expression of heterologous genes in plant systems: New possibilities

Vyacheslavova, A. O.; Berdichevets, I. N.; Tyurin, A. A.; Shimshilashvili, Kh. R.; Mustafaev, O. N.; Goldenkova-Pavlova, I. V.

doi:10.1134/s1022795412110130

Cited by 17 publications

(21 citation statements)

References 60 publications

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“…Rhizobium radiobacter ( Agrobacterium tumefaciens ) mediated methods or particle bombardment are the preferred DNA transfer techniques [10]. To achieve high frequencies of plant regeneration, Cheng et al .…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of the GmESR1 gene associated with soybean regeneration

Zhang

et al. 2017

PLoS ONE

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Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated genes. Sequence analysis showed that GmESR1 contained a conserved 57 amino acid APETALA 2 (AP2)/ETHYLENE RESPONSE FACTOR (ERF) DNA-binding domain. The relative expression level of GmESR1 was highest in young embryos, flowers and stems in the soybean cultivar ‘Dongnong 50’. To examine the function of GmESR1, transgenic Arabidopsis (Arabidopsis thaliana) and soybean plants overexpressing GmESR1 were generated. In Arabidopsis, overexpression of GmESR1 resulted in accelerated seed germination, and seedling shoot and root elongation. In soybean overexpression of GmESR1 also led to faster seed germination, and shoot and root elongation. GmESR1 specifically bound to the GCC-box. The results provide a foundation for the establishment of an efficient and stable transformation system for soybean.

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Section: Introductionmentioning

confidence: 99%

Functional analysis of the GmESR1 gene associated with soybean regeneration

Zhang

et al. 2017

PLoS ONE

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“…A lot of ways to increase the level of foreign gene expression in transgenic plants is developed; they are very fully discussed in the reviews [17,30,31]. Among them are the optimization of the codon composition of the target sequence, the usage of strong promoters, the addition of introns or regions of binding with the nuclear matrix (SAR), and others.…”

Section: Transgenic Plantsmentioning

confidence: 99%

State of research in the field of the creation of plant vaccines for veterinary use

Permyakova

Уварова

Дейнеко

2015

Russ J Plant Physiol

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Transgenic plants as an alternative of costly systems of recombinant immunogenic protein expres sion are the source for the production of cheap and highly efficient biotherapeuticals of new generation, including plant vaccines. In the present review, possibilities of plant system application for the production of recombinant proteins for veterinary use are considered, the history of the "edible vaccine" concept is briefly summarized, advantages and disadvantages of various plant systems for the expression of recombinant immu nogenic proteins are discussed. The list of recombinant plant vaccines for veterinary use, which are at differ ent stages of clinical trials, is presented.Abbreviations: TMV-tobacco mosaic virus; TSP-total soluble protein.

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“…For example, a full understanding of the physiological role of a gene involved in a certain biological process requires in vivo genetic, molecular, and physiological–biochemical analyses. Before the advent of genome-wide sequencing, the functions of genes were analyzed using a “direct” genetics approach, namely, by comparison of the phenotypic or physiological effects of the natural or induced mutations in the sequence of a target gene [ 5 ]. Recently, the availability of genomic and transcriptomic data together with the designed efficient technologies of genetic modification of almost any plant species allows the gene function to be studied using the so-called “inverse” genetics: from the gene to the phenotype.…”

Section: Introductionmentioning

confidence: 99%

“…This approach implies that the function of a gene in plants is studied either by varying its expression followed by analysis of the corresponding phenotype or by complementing the mutation, as a rule, using the models of T-DNA of induced mutants. The expression of a target gene can be varied using two main approaches: (i) by a complete or a partial “switch-off” of its expression, for example, using T-DNA mutations or RNA interference (RNAi) or (ii) via overexpression by producing transgenic plant lines that constitutively express the target gene at a very high level [ 5 ]. According to experimental data, such variations in gene expression can give abnormal phenotypes allowing for identification of more specific or finer effects in the function of the target gene [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The use of plant genetic engineering technologies, i.e., construction of transgenic plants, has made it possible to get unique experimental models of transgenic plants the manifold molecular and physiological–biochemical analysis of which gave unprecedented experimental confirmations for the functional roles of numerous plant genes [ 3 , 4 , 5 ]. Nevertheless, the main limitations of using the technology for obtaining transgenic plants are: (i) dependence of the transformation efficiency on the plant genotype; and (ii) considerable material and time expenditures when constructing plant transgenic lines, including selection of true transgenic individuals among a tremendous number of primary transformants, confirmation of integration of the transferred sequences and of the events of integration of a single copy into the plant genome, determination of the expression level for both mRNA and protein product, and so on ( Figure 1 I).…”

Section: Introductionmentioning

confidence: 99%

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Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions

Tyurin

Suhorukova

Кабардаева

et al. 2020

Plants

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A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and concurrently effective approaches allowing for the insight into the physiological role of gene products. Numerous studies have convincingly demonstrated the efficacy of transient expression strategy for characterization of the plant gene functions. The review goals are (i) to consider the advantages and limitations of different plant systems and methods of transient expression used to find out the role of gene products; (ii) to summarize the current data on the use of the transient expression approaches for the insight into fine mechanisms underlying the gene function; and (iii) to outline the accomplishments in efficient transient expression of plant genes. In general, the review discusses the main and critical steps in each of the methods of transient gene expression in plants; areas of their application; main results obtained using plant objects; their contribution to our knowledge about the fine mechanisms of the plant gene functions underlying plant growth and development; and clarification of the mechanisms regulating complex metabolic pathways.

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Expression of heterologous genes in plant systems: New possibilities

Cited by 17 publications

References 60 publications

Functional analysis of the GmESR1 gene associated with soybean regeneration

Functional analysis of the GmESR1 gene associated with soybean regeneration

State of research in the field of the creation of plant vaccines for veterinary use

Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions

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