Expression of Heat Shock Proteins by Heat Stress in Soybean

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Heat stress is one of the factors disturb productivity and growth of plants. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean.

Transcriptome analysis reveals plasticity in gene regulation due to environmental cues in Primula sikkimensis, a high altitude plant species

“…However, our result revealed that HSP20 and HSP70 were down regulated by heat stress at BA site. As HSPs have been shown to be expressed more under heat stress over short time periods [48, 49] it seemed that in our study HSP20 and HSP70 genes might had responded for short time period after transplanting plants under heat stress at BA site but decreased with time.…”

Effect of Heat Stress on Seed Protein Composition and Ultrastructure of Protein Storage Vacuoles in the Cotyledonary Parenchyma Cells of Soybean Genotypes That Are Either Tolerant or Sensitive to Elevated Temperatures

“…Earlier studies that focused on transcriptome profiling indicated that heat shock proteins may play an important role in improving the tolerance of soybean subjected to heat stress [ 21 , 22 ]. To examine if different growing temperatures had any effect on heat shock protein (HSP) accumulation, we conducted western blot analysis using antibodies specific for HSP70 and HSP17.6.…”

“…Earlier studies that focused on transcriptome profiling indicated that heat shock proteins may play an important role in improving the tolerance of soybean subjected to heat stress [21,22]. To examine if different growing temperatures had any effect on heat shock protein (HSP) accumulation, The superimposed color images generated by Delta2D software facilitates the identification of differences between the two treatments where yellow demonstrates similar protein quantities in both growing temperatures.…”

Improvement of heat stress tolerance in soybean (Glycine max L), by using conventional and molecular tools

“…As a result, small HSP families were found to be upregulated and expressed in prolonged heat stress conditions. These findings provided valuable information about heat tolerance in soybean ( Song et al, 2017 ). Heat shock TFs play a key role in helping soybean plants in extreme heat stress episodes.…”

“…Soybean plants with enhanced tolerance to heat stress showed overexpression of GmEF8 in hairy roots and had a high proline level compared to plants with control hairy roots ( Zhang et al, 2022a ). Earlier, Song et al (2017) performed an RNA-seq analysis using two soybean varieties under extreme stress. They analyzed the transcripts and obtained 2,458 differentially expressed genes.…”