Expression of heat shock protein 70 in nasopharyngeal carcinomas: different expression patterns correlate with distinct clinical prognosis

Abstract

BackgroundHeat shock protein 70, a stress protein, has been implicated in tumor progression. However, its role in nasopharyngeal carcinoma (NPC) progression has not yet been clearly investigated.MethodsImmunohistochemistry (IHC) was employed to examine the expression patterns of Hsp70, human leukocyte antigen –A (HLA-A) in NPC tissue samples.ResultsThe expression of Hsp70 exhibited different spatial patterns among nuclear, membrane and cytoplasm in 507 NPC tumor tissues. Kaplan-Meier survival analysis demonstrated that different Hsp70 expression patterns are correlated with different patient outcomes. High membranal and cytoplasmic levels of Hsp70 predicted good survival of patients. In contrast, high nuclear abundance of Hsp70 correlated with poor survival. Moreover, the membranal and cytoplasmic levels of Hsp70 were positively correlated with levels of the MHC I molecule HLA-A.ConclusionsDifferent Hsp70 expression patterns had distinct predictive values. The different spatial abundance of Hsp70 may imply its important role in NPC development and provide insight for the development of novel therapeutic strategies involving immunotherapy for NPC.

Correlation Between Intracellular Heat Shock Protein 70 Expression and Cervical Lymph Nodes Enlargement in Nasopharyngeal Carcinoma

“…Recent data indicate that the intracellular Hsp70 is regulated in nasopharyngeal carcinoma who had metastases and its expression showed metastasis of cancer. The study by Cai et al (2012) suggest that Hsp70 intranuclear positively correlated with the classification T, N, recurrence, metastasis, clinical stage, and the type of histopathology. Intracellular extracellular Hsp70 is known as a facilitator of the immune response that can interact with receptors on the cell antigens that cause a reaction Hsp70, peptides and antigens.…”

“…Research in China by Cai et al (2012) states that there are differences in the expression of Hsp70 intracellular with staging in patients with NPC, which is at stage III + IV expression of Hsp70 in intracellular larger 6.034 compared to stage I + II .…”

“…Intracellular Hsp70 has the role of anti-apoptosis in caspase independent pathway by binding to AIF is released from mitochondria, therefore restricting translocation into the nucleus (Arya et al 2007). Intracellular Hsp70 can occur translocation from the cytoplasm to the nucleus or nucleolus under conditions of heat stress and serves as a protective barrier against stress-mediated apoptosis and DNA damage, resulting in increased survival of tumor cells (Cai et al 2012).…”

“…In head and neck malignancies in Indonesia, NPC ranked first with a frequency of about 60% (Adham et al 2012). Research in China states that there are differences in the expression of Hsp70 with the staging in patients with NPC, which is at stage III + IV expression of Hsp70 6.034 greater than stage I + II (Cai et al 2012). Another study states that there are differences in the expression of Hsp70 in stage I: II: III: IV is 3: 4: 6: 9 .…”

Molecular classification and molecular targeted therapy of cancer

“…We also detected cancer stem cells in NPC and proposed that genomic instability and DNA damage are among the main causes of cancer stem cell formation [2,3]. In fact, the expressions of many important genes, including p63, RASSF1A, VEGF, CCND1, and Hsp70, significantly differ in NPC tissue obtained from different patients, which we believe will affect the biological behaviors of tumors [4][5][6][7][8]. We further found that immune cells remarkably differ among NPC patients and that the expressions of various immune-related genes in tumor tissue are correlated with disease prognosis [9][10][11].…”