Expression of Heat Shock Protein 70 and Heat Shock Cognate 70 Messenger RNAs in Rat Cortex and Cerebellum After Heat Shock or Amphetamine Treatment

Abstract

The expression of strictly inducible hsp70 mRNAs and constitutively expressed hsc70 mRNAs was compared in cerebellum and cerebral cortex of control rats, heat-shocked rats, and rats made hyperthermic with amphetamine. An hsc70-specific oligonucleotide probe identified a 2.55-kb mRNA in cerebellum and cerebral cortex of all rats. An hsp70-specific oligonucleotide probe identified a 3.05-kb mRNA and a 3.53-kb mRNA in cerebellum and cerebral cortex of heat-shocked and amphetamine-treated rats, but not in control rats. Quantitation demonstrated that both hsp70 and hsc70 mRNA levels, relative to 18S rRNA levels, were increased following each treatment. The relative levels of both mRNAs were higher in cerebellum than in cerebral cortex. In amphetamine-treated rats, hsc70 mRNA relative levels increased at body temperatures greater than 39 degrees C, whereas hsp70 mRNA synthesis was induced at temperatures greater than 40 degrees C. Total thermal response values and relative levels of both mRNAs were compared. The results suggested that both the transcription and turnover of hsp70 mRNAs differed between cerebellum and cerebral cortex. At equivalent total thermal response values, amphetamine-treated rats had higher relative levels of hsp70 mRNAs than heat-shocked rats, suggesting that amphetamine enhanced the induction of hsp70 mRNAs.

Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses

“…A similar case was also observed in flesh fly Sarcophaga crassipalpis, where Hsp70 was significantly up-regulated under heat shock in nondiapausing flies, while the cognate Hsc70 was not affected (Rinehart et al 2000). However, there are also reports to show that the transcription of Hsc70 in the rat cortex and cerebellum was up-regulated after heat shock, but the extent of variation is much lower than that of Hsp70 (Miller et al 1991). The rapid reaction of FcHsp70 might protect shrimp from acute stresses, during which a large number of denatured proteins are necessary to be chaperoned with Hsps in the cells, and then to reduce the overload of unfolded proteins in the cells .…”

Heat Shock Protein 70 Suppresses Astroglial-inducible Nitric-oxide Synthase Expression by Decreasing NFκB Activation

“…However, HSP expression can be induced in these cells in vitro (40,41) as well as in vivo (42), and HSP expression can be protective in several neuropathologies (30). We have therefore tested the possibility that the HS response can reduce and/or prevent astroglial NOS-2 expression.…”

Prolonged Expression of hsp70 mRNA following Transient Focal Cerebral Ischemia in Transgenic Mice Overexpressing CuZn-Superoxide Dismutase

“…The slides were hy bridized at 37°C for 18 h with 1 x 106 cpm of a 35S-labeled synthetic oligonucleotide (30-mer) probe that corre sponds to highly conserved amino acids 122-129 near the 5'-end of the human hsp70 coding sequence (Hunt and Morimoto, 1985). This sequence has been reported to be specific for the inducible hsp70 gene (Miller et al, 1991) and has been used to show that hsp70 mRNA is not ex pressed at detectable levels in most normal rodent cells (Marini et al, 1990;Nowak and Bond, 1990). After hy bridization, the sections were rinsed in 1 x standard sa line citrate (SSC) (150 mM sodium chloride, 15 mM so dium citrate, pH 7.4) at 52°C for 60 min with several changes of 1 x SSC, dehydrated, and exposed to Kodak SB-5 film for a week.…”