Expression of growth genes in response to glycerol use in Japanese quail diets

Gasparino, Eliane; Neto, Adhemar Rodrigues Oliveira; Vesco, Ana Paula Del; Pires, Alexandre Vaz; Batista, Eliany; Voltolini, Débora Marques; Souza, K.R.S.

doi:10.4238/2012.august.31.3

Cited by 6 publications

(4 citation statements)

References 11 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, where Δ Ct = Ct (detected genes) -Ct (β-actin) and Ct represents threshold cycle number (Gasparino et al, 2012).…”

Section: Rna Extraction and Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation

Wang¹,

Wang²,

Jiang³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Pancreatic adenocarcinoma is a lethal disease because it is inoperable at the time of diagnosis. Therefore, the search for new therapeutic approaches is critical. The abnormal expression of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) pathway and alteration in epigenetic modification (DNA methylation and acetylation of histones) is a common feature in the majority of human pancreatic adenocarcinomas. Because DNA methyltransferase levels are regulated by the MEK pathway, we examined the effects of an MEK inhibitor, PD98059, on the action of DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-aza-dC), the epigenetic agent in the pancreatic cell line CFPAC1. Our results showed that PD98059 significantly potentiated the capability of 5-azadC to induce a cessation of cell proliferation concomitant with cell cycle arrest. We also observed an increase in tumor suppressor gene expression associated with the efficacy of treatment with PD98059 and 5-aza-dC. Further studies explored the molecular mechanisms by which . We found that 5-aza-dC induced acetylation of histone H3 on the p21 WAF1 gene promoter and demethylation status on the p21 WAF1 gene promoter region. These effects were strikingly enhanced by the concomitant blockade of the MEK pathway. Furthermore, knockdown of p21 WAF1 by small interfering RNA rescued human pancreatic cancer cells from 5-aza-dC-mediated growth inhibition. Taken together, our results show that the MEK inhibitor enhanced the effects of 5-aza-dC in human pancreatic cancer cells. These results suggest that the MEK signal pathway may be a potential target for pancreatic cancer therapy.

show abstract

“…, where Δ Ct = Ct (detected genes) -Ct (β-actin) and Ct represents threshold cycle number (Gasparino et al, 2012).…”

Section: Rna Extraction and Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation

Wang¹,

Wang²,

Jiang³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Such is observed when there is malnutrition, fasting or deficient absorption of nutrients, where GHR mRNA expression is reduced (Straus and Takemoto, 1990). Considering quail performance and the expression of the genes for GH and IGF-I, Gasparino et al, 2012b considered that 4% glycerol could be used in quail feed without any harmful effects. On the other hand, we found that birds fed 8% glycerol had higher GHR mRNA expression relative to those fed 12% glycerol, suggesting that it is possible to include 8% glycerol in Japanese quail diets with no reduction in GHR mRNA expression.…”

Section: Discussionmentioning

confidence: 99%

Effect of glycerol on GHR and IGF-1 gene expression in breast muscle and on growth of Japanese meat quails

Silva

Gasparino²,

Voltolini³

et al. 2013

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. We evaluated messenger RNA (mRNA) expression of the growth-hormone (GHR) and insulin-like growth factor (IGF-1) genes in 28-day-old Japanese meat quails fed diets containing 0, 8, or 12% dietary glycerol in substitution of corn. Total RNA was extracted from the breast muscle and the DNA was amplified with specific primers using real-time PCR. Feed conversion ratio and feed intake were evaluated. The birds fed 8 and 12% glycerol presented higher IGF-1 mRNA expression [0.059 and 0.049 arbitrary units (AU), respectively] relative to those not fed with glycerol (0.029 AU), while 12% glycerol reduced GHR mRNA expression (0.022 AU). Dietary inclusion of 8% glycerol promoted similar performance results (feed conversion) as the diet with no glycerol. We conclude that inclusion of glycerol in the diet affects GHR and IGF-1 gene expression in Japanese meat quails. However, considering the performance results 3857©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (3): 3856-3861 (2013) Effect of glycerol on GHR and IGF-1 gene expression and the expression of the GHR and IGF-1 genes, 8% glycerol may be safely included in the diet of meat quails.

show abstract

“…Our results are in line with the findings of Erol et al (2009). Similarly, Gasparino et al (2012) demonstrated that glycerine had no statistical effect on Japanese quail BWG. Cerrate et al (2006) assured that glycerol had no significant effect on broiler BWG.…”

Section: Growth Performancementioning

confidence: 99%

Impact of increasing crude glycerine levels in diet on growth, carcass traits, body measurements and blood cholesterol in growing Japanese quails

Arif¹,

Hayat²,

Sohail³

et al. 2017

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Expression of growth genes in response to glycerol use in Japanese quail diets

Cited by 6 publications

References 11 publications

Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation

Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation

Effect of glycerol on GHR and IGF-1 gene expression in breast muscle and on growth of Japanese meat quails

Impact of increasing crude glycerine levels in diet on growth, carcass traits, body measurements and blood cholesterol in growing Japanese quails

Contact Info

Product

Resources

About