Expression of green fluorescent protein in the ureteric bud of transgenic mice: A new tool for the analysis of ureteric bud morphogenesis

Srinivas, Shankar; Goldberg, Michael R.; Watanabe, Tomoko; D’Agati, Vivette D.; Al‐Awqati, Qais; Costantini, Frank

doi:10.1002/(sici)1520-6408(1999)24:3/4<241::aid-dvg7>3.0.co;2-r

Cited by 212 publications

(158 citation statements)

References 27 publications

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“…b: Time-lapse imaging of ureteric bud branching morphogenesis in kidneys expressing a GFP gene controlled by the Hoxb7 promoter, which results in specific expression throughout the Wolffian duct and ureteric bud epithelium. (73) Top row, a kidney cultured in normal medium; bottom row, a kidney cultured in the presence of an inhibitor of the Erk MAP kinase pathway. (57) The Erk inhibitor reduced the rate of branching, resulting in longer segments and fewer tips.…”

Section: The Role Of Localized Gdnf/ret Signaling In Ureter Formationmentioning

confidence: 99%

“…Wnt7b and collagen XVIII). (59,71) These are not two entirely separate lineages, as shown by studies using chimeric kidneys, in which some cells carried a Hoxb7/GFP marker gene, which is expressed only in ureteric bud cells (73) and allows their fates to be followed by time lapse imaging of organ cultures. (74) Cells that were initially located in the tip of an elongating branch gave rise to daughter cells, some of which remain at the tip, and others of which were left behind in the forming trunk (Fig.…”

Section: The Role Of Localized Gdnf/ret Signaling In Ureter Formationmentioning

confidence: 99%

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GDNF/Ret signaling and the development of the kidney

2006

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SummarySignaling by GDNF through the Ret receptor is required for normal growth of the ureteric bud during kidney development. However, the precise role of GDNF/Ret signaling in renal branching morphogenesis and the specific responses of ureteric bud cells to GDNF remain unclear. Recent studies have provided new insight into these issues. The localized expression of GDNF by the metanephric mesenchyme, together with several types of negative regulation, is important to elicit and correctly position the initial budding event from the Wolffian duct. GDNF also promotes the continued branching of the ureteric bud. However, it does not provide the positional information required to specify the pattern of ureteric bud growth and branching, as its site of synthesis can be drastically altered with minimal effects on kidney development. Cells that lack Ret are unable to contribute to the tip of the ureteric bud, apparently because GDNF-driven proliferation is required for the formation and growth of this specialized epithelial domain.

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Section: The Role Of Localized Gdnf/ret Signaling In Ureter Formationmentioning

confidence: 99%

Section: The Role Of Localized Gdnf/ret Signaling In Ureter Formationmentioning

confidence: 99%

GDNF/Ret signaling and the development of the kidney

2006

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“…Among these is the production of therapeutic specific proteins in milk or urine (Chalfie et al, 1994;Chiocchetti et al, 1997;Zhou et al, 1997;Baumann et al, 1998) and the labelling of some specific tissues such as the ureteric bud (Srinivas et al, 1999). GFP can also be used as a biomarker for selection of transfected cells (Chan et al, 1999;Arat et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The use of green ﬂuorescent protein (GFP) to select bovine embryos

Duszewska¹,

Козикова²,

Szydlik³

et al. 2003

J. Anim. Feed Sci.

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A major factor limiting the transgenesis in domestic animals is the inefficiency of maintaining large numbers of recipients carrying nontransgenic foetuses. The objectives of this study were: 1. to determine the influence of green fluorescent protein (GFP) construct injection on the development of bovine embryos, 2. to identify and select the GFP positive bovine embryos, and 3. to determine the rate of mosaicism in transgenic embryos. Cattle oocytes were matured and fertilised in vitro and zygotes were microinjected with pCX-EGFP construct consisting of CMV-IE enhancer, chicken β-actin promoter, cDNA of GFP (EGFP-732 bp) and rabbit β-globin polyadenylation sequences. Embryos from control (64) and microinjected (198) groups were cultured in vitro. After 168 h of culture, morula and blastocysts were observed in 39.06% of control and in 23.23% of injected group. We obtained three GFP positive embryos (1.51% of injected zygotes and 6.52% of morulae/blastocysts). One of them was 100, second 75 and third 25% GFP positive (66.7% of mosaicism). Use of gfp gene reporter to select bovine embryos is useful method to increase transgenic offspring, because GFP marker allows to choice only transgenic embryos and transfer them to recipients.

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“…Epithelial cells in the ureteric bud can be labeled with GFP using the HoxB7 promoter, creating a nice model for studying branching patterns (Srinivas et al, 1999). Recently, a role for the glial-derived neurotrophic factor (GDNF)/Ret pathway in branching has been reported (Shakya et al, 2005).…”

Section: Imaging Dynamic Changes In Cell Behavior In Explanted Tissuesmentioning

confidence: 99%

Multimodal imaging of mouse development: Tools for the postgenomic era

Dickinson

2006

Developmental Dynamics

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With the sequence of the mouse genome known, it is now possible to create or identify mutations in every gene to determine the molecules necessary for normal development. Consequently, there is a growing need for advanced phenotyping tools to best understand defects produced by altering gene function. Perhaps nothing is more satisfying than to directly observe a process in action; to disturb it and see for ourselves how the process changes before our very eyes. No doubt, this desire is what drove the invention of the very first microscopes and continues to this day to fuel progress in the field of biological imaging. Because mouse embryos are small and develop embedded within many tissue layers within the nurturing environment of the mother, directly observing the dynamic, micro-and nanoscopic events of early mammalian development has proven to be one of the greater challenges for imaging scientists. Here, I will review some of the imaging methods being used to study mouse development, highlighting the results obtained from imaging.

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Expression of green fluorescent protein in the ureteric bud of transgenic mice: A new tool for the analysis of ureteric bud morphogenesis

Cited by 212 publications

References 27 publications

GDNF/Ret signaling and the development of the kidney

GDNF/Ret signaling and the development of the kidney

The use of green ﬂuorescent protein (GFP) to select bovine embryos

Multimodal imaging of mouse development: Tools for the postgenomic era

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