Expression of glycoprotein gp43 in stage-specific forms and during dimorphic differentiation ofParacoccidioides brasiliensis

Abstract

Expression of the 43 kDa glycoprotein (gp43) was analysed in several Paracoccidioides brasiliensis isolates. Using one-and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. In two strains, in both yeast and mycelium cells, gp43 was present, whereas expression was restricted to the yeast phase of two other strains. The clinical implications of this phase-specific gp43 expression are uncertain.

Morphological, Biochemical and Molecular Approaches for Comparing Typical and Atypical Paracoccidioides brasiliensis Strains

“…We do not discard the possibility that the atypical strain Pb IOC 1059 has an impairment in the secretion of the intracellular GP43, as we did not analyze the cellular content of GP43. According to Mattar-Filho et al (1997), the maximum expression of GP43 occurred within 12 days of cellular differentiation. Therefore, a lower production of GP43 could last for lesser time, such that by day 12 no more glycoprotein would be available for detection.…”

Characterization of Paracoccidioides brasiliensis by FT-IR spectroscopy and nanotechnology

“…This gene encodes a glycosilated protein and a major antigen that react with 100% of sera from patients with PCM [22]. GP43 glycoprotein binds to murine laminin to entail an increased invasiveness and destruction of tissues infected [23], and is abundantly detected in yeast phase [24].…”