Expression of glucosamine trisaccharides on the rat uterine surface is altered by clomiphene citrate. – III. Relationship with implantation regimes and pregnancy

Hosie, Margot J.; Terry, Vera; Murphy, Christopher R.

doi:10.1078/0065-1281-00732

Cited by 7 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was found to be dose-specific, and the 15 mg/ kg anastrozole dose superovulated and achieved pregnancy the most; therefore, this dose was used in the subsequent experiments in this study. The CC dose was adopted from earlier studies (Hosie et al, 2003) and used as a comparison.…”

Section: Rationale For the Dosesmentioning

confidence: 99%

See 1 more Smart Citation

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

et al. 2020

View full text Add to dashboard Cite

An alternative hyper-ovulator inducer to replace clomiphene citrate (CC) is needed as it is unsuitable for women with polycystic ovarian syndrome and is associated with low pregnancy rates. Anastrozole is an effective hyper-ovulator inducer, but has not been well researched. In order to determine the effectiveness of anastrozole as a hyper-ovulator inducer and to an extent compare it with CC in similar situations, this study ascertained the effects of these drugs on the expression of the focal adhesion proteins, paxillin and FAK, which are uterine receptivity markers in the surface luminal uterine epithelial cells of day 1 and day 6 pregnant Wistar rats. The results show that paxillin is localized in focal adhesions at the base of the uterine epithelial cells at day 1 of pregnancy whereas at day 6, paxillin disassembles from the basal focal adhesions and localizes and increases its expression apically. FAK is faintly expressed at the basal aspect of the uterine epithelial cells while moderately expressed at the cell-to-cell contact at day 1 in all groups from where it disassembles and relocates apically and becomes more intensely expressed at day 6 of pregnancy in untreated and anastrozole treated rats. Although paxillin is localized apically at day 6, its expression is significantly down-regulated with CC treatment suggesting its interference with the implantation process. These findings seem to suggest that anastrozole could favor implantation.

show abstract

Section: Rationale For the Dosesmentioning

confidence: 99%

“…All injections were intraperitoneal. The 15 mg/kg body weight anastrozole dose was found to be the optimal dose for the preliminary dose response study whereas the 1.25 mg/kg of body weight, CC was used which was adopted from previous studies (Hosie et al, 2003).…”

Section: Drug Preparation and Administrationmentioning

confidence: 99%

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Dose rates in the preliminary work were started at 1 mg/kg. The chlomiphene citrate dose of 1.25 mg/kg (Sigma-Aldrich Co., St. Louis, MO, USA) was adopted from earlier studies (Hosie et al, 2003) and used as a comparison.…”

Section: Methodsmentioning

confidence: 99%

“…Disassembly of the basal focal adhesions along the uterine epithelial cells makes cells less adherent to the underlying basement membrane, and this facilitates their removal, enabling embryonic trophoblast cells to invade the endometrial decidual cells beneath. Since previous studies demonstrated that focal adhesion proteins are more likely regulated in uterine epithelia to change cell behavior (Kaneko et al, 2009), and that super-ovulatory drugs can alter expression of key molecules in the uterine epithelial cells during implantation (Hosie et al, 2003), anastrozole may act on focal adhesion proteins during implantation. To date, anastrozole regulation of focal adhesion proteins in the uterine epithelial cells during implantation has not been established.…”

Section: Introductionmentioning

confidence: 99%

“…Anastrozole is clinically effective in ovulation induction, but it has not been well researched (Tredway & Schertz, 2011). Investigating and understanding the effects of anastrozole on the endometrium at the time of implantation in vivo will enable manipulation of uterine receptivity to control fertility and to improve the outcome of assisted reproductive procedures (Paria et al, 2001;Hosie et al, 2003;Karaer et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Focal Adhesion Proteins, Vinculin and Integrin β5, During Early Pregnancy in Rat Uterine Epithelial Cells: Anastrozole Favors their Normal Distribution

et al. 2018

View full text Add to dashboard Cite

SUMMARY:An alternative superovulator to replace clomiphene citrate is needed as clomiphene citrate is associated with low pregnancy rates. Anastrozole is an effective superovulator, but it has not been well researched. In order to determine the effectiveness of anastrozole as a superovulator and to compare it with clomiphene citrate in similar situations, this study ascertained the effects of these drugs on the expression of the focal adhesion proteins, vinculin and integrin β5, which are uterine receptivity markers, in the uterine epithelial cells of day 1 and day 6 pregnant Wistar rats. The results show that vinculin and integrin β5 are co-localized at the base of the uterine epithelium at day 1 of pregnancy whereas at day 6, they disassemble from the basal focal adhesions and co-localize and significantly increase their expression apically (p≤0.0001). Moreover, there is a significant difference in the protein expression levels of vinculin and integrin β5 in uterine luminal epithelial cells between untreated (control) and chlomiphene citrate treated rats (p≤0.0001), anastrozole and chlomiphene citrate treated rats at day 6 (p≤0.0001) suggesting the interpretation that anastrozole seems to enhance their expression in order to perhaps assist in the implantation process of the blastocyst. The immunofluorescence experiments agree with the vinculin and integrin β5 gene expression findings in which at day 6 of pregnancy, vinculin and integrin β5 gene expression are significantly upregulated in uterine luminal epithelial cells in the anastrozole treated group relative to the calibrator sample (p≤0.0001). These findings suggest that anastrozole is implantation friendly.

show abstract

Anastrozole is a dose-specific superovulator and favors implantation in rats: a prospective study

Mwakikunga

Hosie

2015

Cell Tissue Res

View full text Add to dashboard Cite

An alternative superovulator to replace clomiphene citrate (CC) is needed as it is unsuitable for women with polycystic ovarian syndrome and is associated with low pregnancy rates. Anastrozole is an effective superovulator, but has not been well researched. The aim of this study was to determine the optimal dose of anastrozole as a superovulator and ascertain its effects on implantation and uterine ultrastructure during early pregnancy in Wistar rats using scanning electron microscopy. The uterine morphological characteristics which were studied in day 1 and 6 pregnant rats include microvilli density, length, surface "beads", surface glycocalyx, cell borders and apices, uterine surface fording and large surface protrusions. A significant increase in implantation sites is seen in the 15 mg/kg anastrozole group, compared to control. Day 1 and 6 anastrozole groups have similar morphology to the control and different to the CC group. At day 6, large surface protrusions are mostly noted but not limited to anastrozole-treated rats; anastrozole also appears to retain glycocalyx to some extent. The increased number of implantation sites in the 15 mg/kg anastrozole group suggests that this dose superovulates and favors implantation. Anastrozole is probably dose-/species-specific and additionally the surface uterine morphology suggests that anastrozole is implantation friendly.

show abstract

Expression of glucosamine trisaccharides on the rat uterine surface is altered by clomiphene citrate. – III. Relationship with implantation regimes and pregnancy

Cited by 7 publications

References 42 publications

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

Focal Adhesion Proteins, Vinculin and Integrin β5, During Early Pregnancy in Rat Uterine Epithelial Cells: Anastrozole Favors their Normal Distribution

Anastrozole is a dose-specific superovulator and favors implantation in rats: a prospective study

Contact Info

Product

Resources

About

Expression of glucosamine trisaccharides on the rat uterine surface is altered by clomiphene citrate. – III. Relationship with implantation regimes and pregnancy

Cited by 7 publications

References 42 publications

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

Anastrozole Promotes Implantation by Altering the Expression of Paxillin and FAK in Rat Luminal Uterine Epithelium

Focal Adhesion Proteins, Vinculin and Integrin &#946;5, During Early Pregnancy in Rat Uterine Epithelial Cells: Anastrozole Favors their Normal Distribution

Anastrozole is a dose-specific superovulator and favors implantation in rats: a prospective study

Contact Info

Product

Resources

About

Focal Adhesion Proteins, Vinculin and Integrin β5, During Early Pregnancy in Rat Uterine Epithelial Cells: Anastrozole Favors their Normal Distribution