Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility

Herath, Shan; Lilly, Sonia T.; Santos, N.R.; Gilbert, R.O.; Goetze, Leopold; Bryant, Clare E.; White, John O.; Cronin, James G.; Sheldon, I. Martin

doi:10.1186/1477-7827-7-55

Cited by 157 publications

(142 citation statements)

References 34 publications

(72 reference statements)

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“…calcium-binding proteins S100A8, S100A9 and S100A12 and receptors TLR4 and IL1R (Gabler et al 2009, Herath et al 2009b, Wathes et al 2009. Furthermore, there are temporal changes in the expression of transcripts for IL1B, IL6, CXCL8, TNF, CXCL5, HP and PTGS2 during the postpartum period, with peak expression on day 17 after parturition .…”

Section: R45mentioning

confidence: 94%

See 1 more Smart Citation

Innate immunity and inflammation of the bovine female reproductive tract in health and disease

Sheldon¹,

Cronin²,

Healey³

et al. 2014

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127

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Mammalian reproductive physiology and the development of viviparity co-evolved with inflammation and immunity over millennia. Many inflammatory mediators contribute to paracrine and endocrine signalling, and the maintenance of tissue homeostasis in the female reproductive tract. However, inflammation is also a feature of microbial infections of the reproductive tract. Bacteria and viruses commonly cause endometritis, perturb ovarian follicle development and suppress the endocrine activity of the hypothalamus and pituitary in cattle. Innate immunity is an evolutionary ancient system that orchestrates host cell inflammatory responses aimed at eliminating pathogens and repairing damaged tissue. Pattern recognition receptors on host cells bind pathogen-associated molecular patterns and damage-associated molecular patterns, leading to the activation of intracellular MAPK and NFkB signalling pathways and the release of inflammatory mediators. Inflammatory mediators typically include the interleukin cytokines IL1b and IL6, chemokines such as IL8, interferons and prostaglandins. This review outlines the mechanisms of inflammation and innate immunity in the bovine female reproductive tract during health and disease condition.

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Section: R45mentioning

confidence: 94%

“…Endometrial biopsies collected from healthy and diseased postpartum dairy cows express mRNA for all ten TLRs (Herath et al 2009b). In addition, ex vivo organ cultures of bovine endometrium accumulate IL1b, IL6, IL8, and prostaglandin E 2 when challenged with E. coli or T. pyogenes (Borges et al 2012, Amos et al 2014.…”

Section: R45mentioning

confidence: 99%

Innate immunity and inflammation of the bovine female reproductive tract in health and disease

Sheldon¹,

Cronin²,

Healey³

et al. 2014

Reproduction

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127

100

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“…In addition, systemic factors such as elevated concentrations of serum progesterone (Forde et al 2009a), disease (Gabler et al 2009;Herath et al 2009) as well as dietary status (Coyne et al 2008;Wathes et al 2009) all alter gene expression in the uterine endometrium. These studies indicate that alterations in endometrial gene expression are required to provide an optimum uterine environment for the developing embryo/conceptus.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Beltman

Forde

Furney

et al. 2010

Reprod. Fertil. Dev.

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Abridged title: Endometrial gene expression and metabolic parameters in subfertile beef heifers. AbstractThe objective of this study was to compare the hormonal, metabolic characteristics and endometrial gene expression profiles in beef heifers yielding either a viable or a degenerate embryo on Day 7 after insemination as a means to explain differences in embryo survival.Oestrus was synchronised in cross bred beef heifers (n=145) using a CIDR-prostaglandin protocol and heifers (n=102) detected in standing oestrus (within 24-48 h post CIDR removal) were inseminated 12-18 h after detection of oestrus (Day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Blood samples were collected from Day 4 to Day 7 post oestrus to measure progesterone (on Days 4, 5 and 7), insulin and IGF-I (on Days 4 and 6) and urea concentrations (on Day 7). All animals were slaughtered on Day 7. Uterine pH was also determined. Animals from which an embryo was recovered were classified as either having a viable embryo (morula/blastocyst stage); n=32, or a retarded embryo (arrested at 2-to 16-cell stage); n=19. The overall recovery rate was 64%. There was no significant difference in blood parameters measured or uterine pH at slaughter between the heifers with either a viable or retarded embryo. The relative abundance of 9 transcripts out of 53 tested from the endometrial tissue was different between heifers with a viable embryo or retarded embryo: MOGAT1, PFKB2, LYZ2, SVS8, UHRF1, PTGES, AGPAT4, DGKA and HGPD. Both LYZ2 and UHRF1 are associated with regulation of the immune system. PFKFB2 is a mediator in glycolysis and MOGAT, AGPAT4 and DGKA belong to the triglyceride synthesis pathway. PTGES and HGPD belong to the prostaglandin pathway.Both of these metabolic pathways are important for early embryonic development. In conclusion, retarded embryo development in this study was not related to serum progesterone, IGF-I, insulin or urea concentrations nor to uterine pH at slaughter. However, altered expression of genes involved in the prostaglandin and triglyceride pathways, as well as 2 genes that are closely associated with the regulation of immunity in the endometrium may indicate a uterine component to the retardation of the embryos in these beef heifers.

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“…Therefore, the protective effect of Ge and Di on the decreased endometrial barrier function was continuously examined by challenging PEG cells with bacterial endotoxin O111:B4 E. coli LPSs following 48 h pretreatment with Ge, Di, or vehicle. The bovine endometrial epithelial and stromal cells have been reported to express TLR4 and MyD88 protein, which binds to LPS and stimulates the cellular release of prostaglandins, cytokines, and chemokines that coordinate the immune and physiological responses including alteration of tissue permeability (Davies et al 2008, Herath et al 2009). The expression of toll-like receptors has been identified in the porcine endometrium, although the regulation of TJ barrier function by microorganism has never been indicated (Croy et al 2009(Croy et al , Østrup et al 2010.…”

Section: Discussionmentioning

confidence: 99%

Soy isoflavones improves endometrial barrier through tight junction gene expression

Kiatprasert¹,

Deachapunya²,

Benjanirat³

et al. 2015

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Contamination with bacterial endotoxin causes the disruption of the tight junction (TJ) barrier. We investigated the ameliorative effect of dietary flavonoids genistein (Ge) and daidzein (Di) in normal or lipopolysaccharide (LPS)-induced disruption of epithelial barrier function of the endometrium. Using the immortalized porcine glandular endometrial epithelial cells (PEG), transepithelial electrical resistance (TER) and FITC-dextran flux (FD-4) across the monolayer were measured. The mRNA expression of TJ proteins, zona occludens-1 (ZO1), and claudin-1, -3, -4, -7 and -8 was evaluated by real-time RT-PCR for coinciding effect of Ge or Di occurred at the gene transcription level. The results revealed that Ge and Di altered the TER, depending on times and concentrations. Low concentration (10 K10 M) of both compounds decreased the TER, whereas higher concentrations (10 K8 and 10 K6 M) increased the TER which was not related to the FD-4 flux. The increased TER by Ge or Di was parallel to the induction of claudin-3 and -4 or -8 mRNA expression respectively. With LPS inoculation, all isoflavone treatments inhibited the decreased TER induced by LPS, but only Ge (10 K8 or 10 K6 M) or Di (10 K10 or 10 K6 M) was coincidence with the decreased FD-4 flux. Under this LPS-stimulated condition, some or all examined TJ gene expressions appeared to be promoted by specific concentration of Ge or Di respectively. Our findings suggest that the soy isoflavones treatment could promote and restore the impaired endometrial barrier function caused by LPS contamination.

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Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility

Cited by 157 publications

References 34 publications

Innate immunity and inflammation of the bovine female reproductive tract in health and disease

Innate immunity and inflammation of the bovine female reproductive tract in health and disease

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Soy isoflavones improves endometrial barrier through tight junction gene expression

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