Soy isoflavones improves endometrial barrier through tight junction gene expression

Abstract

Contamination with bacterial endotoxin causes the disruption of the tight junction (TJ) barrier. We investigated the ameliorative effect of dietary flavonoids genistein (Ge) and daidzein (Di) in normal or lipopolysaccharide (LPS)-induced disruption of epithelial barrier function of the endometrium. Using the immortalized porcine glandular endometrial epithelial cells (PEG), transepithelial electrical resistance (TER) and FITC-dextran flux (FD-4) across the monolayer were measured. The mRNA expression of TJ proteins, zona occludens-1 (ZO1), and claudin-1, -3, -4, -7 and -8 was evaluated by real-time RT-PCR for coinciding effect of Ge or Di occurred at the gene transcription level. The results revealed that Ge and Di altered the TER, depending on times and concentrations. Low concentration (10 K10 M) of both compounds decreased the TER, whereas higher concentrations (10 K8 and 10 K6 M) increased the TER which was not related to the FD-4 flux. The increased TER by Ge or Di was parallel to the induction of claudin-3 and -4 or -8 mRNA expression respectively. With LPS inoculation, all isoflavone treatments inhibited the decreased TER induced by LPS, but only Ge (10 K8 or 10 K6 M) or Di (10 K10 or 10 K6 M) was coincidence with the decreased FD-4 flux. Under this LPS-stimulated condition, some or all examined TJ gene expressions appeared to be promoted by specific concentration of Ge or Di respectively. Our findings suggest that the soy isoflavones treatment could promote and restore the impaired endometrial barrier function caused by LPS contamination.

Frontline Science: Two flavonoid compounds attenuate allergic asthma by regulating epithelial barrier via G protein-coupled estrogen receptor: Probing a possible target for allergic inflammation

“…It had been reported that estrogen possessed protective effects on the blood‐brain barrier function in mice and improved the TJs damage 15,35 . Similarly, soy isoflavones had good effects on promoting and restoring LPS‐induced damage to the endometrial barrier function 36 . The role of estrogen signaling in immune regulation had historically been studied in terms of the classical estrogen receptors ERα and ERβ 37 .…”

Kaempherol and Luteolin Decrease Claudin-2 Expression Mediated by Inhibition of STAT3 in Lung Adenocarcinoma A549 Cells

“…Quercetin enhances intestinal barrier in Caco-2 cells through the elevation of claudin-4 [34] and through the assembly of zonula occludens-2, occludin, and claudin-1 [35]. Genistein and daidzein protect lipopolysaccharide-induced disruption of barrier function in glandular endometrial epithelial cells through the elevation of claudin-3, -4, and -8 [36]. In contrast, the effects of flavonoids on the pathophysiological function and expression of claudins in cancer cells have not been examined well.…”

Dietary soy isoflavones alleviate dextran sulfate sodium-induced inflammation and oxidative stress in mice

“…This indicated a beneficial role in intestinal morphologic structure and barrier function. Kiatprasert et al (31) reported that treatment with soy isoflavones may promote and restore the impaired endometrial barrier function by increasing the gene expression of tight junction-associated genes in lipopolysaccharide-induced inflammation.…”