Expression of Gap Junctional Protein Connexins in Human Nasal Epithelium, and Its Contribution to Intercellular Calcium Signalling.

Hama, Takemitsu; Oyamada, Masahito; Dejima, Kenji; Takenaka, Hiroshi; Takamatsu, Tetsuro

doi:10.1267/ahc.33.23

Cited by 3 publications

(4 citation statements)

References 28 publications

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“…Recently, the application of CLSM combined with computer image analysis in the field of cell biology has been expanded. CLSM now enables one to observe the subcellular localization of various biologically active substances utilizing routinely processed light microscopic specimens [1-3, 8,13,16,26,33].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of DAB and Methyl Green Signals on Apoptotic Nuclei by Confocal Laser Scanning Microscopy

Itoh¹,

Umemura

Hasegawa³

et al. 2003

Acta Histochem. Cytochem

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Observations of the fine structures of subcellular organelles and their pathophysiological changes have been made by many investigators by utilizing the many advantageous functions of confocal laser scanning microscopy (CLSM). However, reports of CLSM observations of fine inner structural changes of nuclei are rather rare. The usefulness and value of counterstaining in histochemical staining is widely appreciated. While observing methyl green (MG) nuclear-counterstained sections by CLSM, we found that fluorescence emitted through MG was clearly detected by CLSM. It is generally accepted that MG reacts specifically to double stranded (dbs)-DNA, as was proved by Umemura [30] in our laboratory.In the present study, MG-stained nuclei of the absorptive cells (terminal differentiating cells) of rat intestinal villi were observed by CLSM, and changes in the intensity and distribution of MG staining were examined. The small intestinal epithelial cells were also found to be slowly undergoing apoptosis. The apoptosis is thought to occur during the process of terminal differentiation, which takes about 48 hr. To examine those apoptotic changes, a TUNEL method, in which the 3'ends of fragmented dbs-DNAs are labeled with histochemically detectable dUTP, was employed. The interrelationship between cell functions, which can be detected by various histochemical observations of cytoplasmic substances, and cellular differentiation state, can be elucidated by using MG in CLSM.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of DAB and Methyl Green Signals on Apoptotic Nuclei by Confocal Laser Scanning Microscopy

Itoh¹,

Umemura

Hasegawa³

et al. 2003

Acta Histochem. Cytochem

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“…Since this microscopy combined with computed image analysis was introduced, its applications have expanded at an astonishing rate. CLSM has enabled variable regions of routinely processed light microscopic specimens to be observed not only at the microscopic but also at the subcellular level [18][19][20], because the optical sectioning minimizes flaring and optimizes the fluorescence signals, and images taken by CLSM can be digitized and made available for manipulation and computer-assisted 3D reconstruction [10,13,20,30,40,43,44,53,55].…”

Section: Confocal Laser Scanning Microscopy (Clsm) Configurationmentioning

confidence: 99%

“…Probes for non-confocal laser scanning microscopy (N-CLSM) Non-fluorescent as well as fluorescent signals are detectable by CLSM [1,2,5,13,17,37,52,54].…”

Section: Transmittance and Reflectance Confocal Imagesmentioning

confidence: 99%

“…The application of CLSM to computer-assisted 3D image analysis with immunohistochemistry (IHC) is very exciting because IHC signals taken by CLSM can be digitized, and made available for manipulation and computer-assisted 3D reconstruction. In early experiments, only fluorescent signals were detectable by CLSM [1,5,13,17,29,51,52,54], however, recent innovations have enabled visualization of non-fluorescent probes such as those labeled with peroxidase, which produces a brown, insoluble reaction product with hydrogen peroxide and diaminobenzidine (DAB) [19,46].…”

Section: Introductionmentioning

confidence: 99%

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Confocal Laser Scanning Microscopic Imaging of Subcellular Organelles, mRNA, Protein Products, and the Microvessel Environment.

Itoh

Matsuno

Yamamoto³

et al. 2001

Acta Histochem. Cytochem

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The confocal laser scanning microscope (CLSM) was employed to visualize highly contrasted images of histochemically stained subcellular organelles. In early studies, only fluorescent signals were detectable by CLSM, however, recent innovations have enabled the visualization of non-fluorescent probes such as horseradish peroxidase (HRP)-diaminobenzidine (DAB). The non-fluorescent signals are permanently preserved and allow repeated or retrospective examinations. We applied CLSM to specimens prepared for light microscopy, and visualized subcellular organelles and pituitary hormone mRNA. The images were comparable to those obtained by electron microscopy (EM). We also applied this technique to the observation of tumor angiogenesis and the microvessel environment of hormone-secreting cells. Both the visualization of subcellular organelles, mRNA and protein products, and 3D images of the microvessel environment of hormone-secreting cells are discussed in this review.

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An Anti-Peptide Antibody that Recognized Unexpected Protein. A Case Report.

Matsuzaki

Suzuki

Tanaka

et al. 2000

Acta Histochem. Cytochem

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Expression of Gap Junctional Protein Connexins in Human Nasal Epithelium, and Its Contribution to Intercellular Calcium Signalling.

Cited by 3 publications

References 28 publications

Simultaneous Detection of DAB and Methyl Green Signals on Apoptotic Nuclei by Confocal Laser Scanning Microscopy

Simultaneous Detection of DAB and Methyl Green Signals on Apoptotic Nuclei by Confocal Laser Scanning Microscopy

Confocal Laser Scanning Microscopic Imaging of Subcellular Organelles, mRNA, Protein Products, and the Microvessel Environment.

An Anti-Peptide Antibody that Recognized Unexpected Protein. A Case Report.

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