An Anti-Peptide Antibody that Recognized Unexpected Protein. A Case Report.

Matsuzaki, Toshiyuki; Suzuki, Takeshi; Tanaka, Shigeyasu; Tanaka, Kuniaki

doi:10.1267/ahc.33.361

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Fluorescent Staining Techniques Using the Fluorescent-labele1

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“…1 ) [ 15 , 29 , 32 ]. Special caution should be paid to the possible cross-reactivity of antibodies to the unintended molecules in the tissue cells [ 14 ].…”

Section: Fluorescent Staining Techniques Using the Fluorescent-labelementioning

confidence: 99%

Recent Advances in Fluorescent Labeling Techniques for Fluorescence Microscopy

Suzuki

Matsuzaki

Hagiwara

et al. 2007

Acta Histochem. Cytochem.

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AHCTremendous progress in recent computer-controlled systems for fluorescence and laserconfocal microscopy has provided us with powerful tools to visualize and analyze molecular events in the cells. Various fluorescent staining and labeling techniques have also been developed to be used with these powerful instruments. Fluorescent proteins such as green fluorescent protein (GFP) allow us to directly label particular proteins of interest in living cells. This technique has been extended over a large area of cell biology, and a variety of fluorescent protein-derived techniques have been developed to visualize the functions and conditions of the molecules within living cells. In this review, we summarize the techniques for fluorescent staining and labeling for recent fluorescence microscopy.

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“…1 ) [ 15 , 29 , 32 ]. Special caution should be paid to the possible cross-reactivity of antibodies to the unintended molecules in the tissue cells [ 14 ].…”

Section: Fluorescent Staining Techniques Using the Fluorescent-labelementioning

confidence: 99%