Expression of fos immunoreactivity in some catecholaminergic brainstem neurons in rats following high-altitude exposure

Kaur, Charanjit; You, Y.; Singh, Jeffrey M.; Peng, Caixia; Ling, Eng‐Ang

doi:10.1002/1097-4547(20010101)63:1<54::aid-jnr7>3.0.co;2-x

Cited by 11 publications

(7 citation statements)

References 40 publications

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“…This circumventricular organ is considered to be the chemosensitive portion of the dorsal vagal complex (Saper, 1995) and its connections in rats (Shapiro and Miselis, 1985;Larsen et al, 1991) are evidence of its involvement in the regulation of cardiovascular functions (Ling and Wong, 1987). Our data are consistent with previous studies that detected increased expression of c-fos in this region after exposure to hypobaric hypoxia (Kaur et al, 2001).…”

Section: Discussionsupporting

confidence: 91%

A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

Takada

Sampaio

Allemandi

et al. 2011

Journal of Neuroscience Methods

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Section: Discussionsupporting

confidence: 91%

A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

Takada

Sampaio

Allemandi

et al. 2011

Journal of Neuroscience Methods

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“…Previous data about the catecholaminergic character of c-FOS-positive hypoxia-activated cells in these structures made in adult mammals have showed that no, or very few, c-FOS hypoxia-activated neurons in the NTS are also catecholaminergic, whereas a large portion of c-FOS hypoxia-activated neurons in the VLM are also immunoreactive for TH (Erickson and Millhorn, 1994; Soulier et al, 1997; Teppema et al, 1997). The only data from the literature concerning this issue in newborn animals is the study of c-FOS expression in newborn rats under high-altitude conditions showing that an altitude of 8000 m induces an increase in the number of c-FOS positive cells in the NTS and VLM with a large portion of them in the VLM also immunoreactive for TH (Kaur et al, 2001). Taking into account these data, our results suggest that the effect of hypoxia on the VLM differed in one-day-old mice from other studied newborn or adult mammals.…”

Section: Discussionmentioning

confidence: 99%

Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants

et al. 2016

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We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the nucleus of the solitary tract, ventral reticular nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus nucleus, lateral, and medial parabrachial nucleus, and dorsal subcoeruleus nucleus. In contrast, the hypoglossal nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas ~45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labeled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labeled for serotonin in the raphe magnus nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labeled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by (i) the activation of catecholaminergic cells of the dorsal subcoeruleus nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, (ii) the weak activation of catecholaminergic cells of the ventral reticular nucleus of the medulla, an area involved in hypoxic hyperventilation, and (iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modeling the breathing network of premature infants.

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“…From the neuronal phenotypes studied we detected mainly TH immunoreactive cells which were activated by both types of LIRIs. Previous studies have shown that NE neurons in NTS are activated by visceral and subcutaneous pains (Han et al 2003), abdominal surgery (Bonaz and Taché 1997), highaltitude exposures connected with hypoxia (Kaur et al 2001), immune challenges (Bonnet et al 2009), and osmotic stress, e.g. hypertonic saline injection (Pirnik et al 2004a, b).…”

Section: Discussionmentioning

confidence: 94%

Activation of Different Neuronal Phenotypes in the Rat Brain Induced by Liver Ischemia–Reperfusion Injury: Dual Fos/Neuropeptide Immunohistochemistry

et al. 2010

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The aim of the present study was to reveal the effect of liver ischemia–reperfusion injury (LIRI) on the activity of selected neuronal phenotypes in rat brain by applying dual Fos-oxytocin (OXY), vasopressin (AVP), tyrosine hydroxylase (TH), phenylethanolamine N-methyltransferase (PNMT), corticoliberine (CRH), and neuropeptide Y (NPY) immunohistochemistry. Two liver ischemia–reperfusion models were investigated: (i) single ligation of the hepatic artery (LIRIa) for 30 min and (ii) combined ligation of the portal triad (the common hepatic artery, portal vein, and common bile duct) (LIRIb) for 15 min. The animals were killed 90 min, 5 h, and 24 h after reperfusion. Intact and sham operated rats served as controls. As indicated by semiquantitative estimation, increases in the number of Fos-positive cells mainly occurred 90 min after both liver reperfusion injuries, including activation of AVP and OXY perikarya in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, and TH, NPY, and PNMT perikarya in the catecholaminergic ventrolateral medullar A1/C1 area. Moreover, only PNMT perikarya located in the A1/C1 cell group exhibited increased Fos expression 5 h after LIRIb reperfusion. No or very low Fos expression was found 24 h after reperfusion in neuronal phenotypes studied. Our results show that both models of the LIRI activate, almost by the same effectiveness, a number of different neuronal phenotypes which stimulation may be associated with a complex of physiological responses induced by (1) surgery (NPY, TH, PNMT), (2) hemodynamic changes (AVP, OXY, TH, PNMT), (3) inflammation evoked by ischemia and subsequent reperfusion (TH), and (4) glucoprivation induced by fasting (NPY, PNMT, TH). All these events may contribute by different strength to the development of pathological alterations occurring during the liver ischemia–reperfusion injury.

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Expression of fos immunoreactivity in some catecholaminergic brainstem neurons in rats following high-altitude exposure

Cited by 11 publications

References 40 publications

A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants

Activation of Different Neuronal Phenotypes in the Rat Brain Induced by Liver Ischemia–Reperfusion Injury: Dual Fos/Neuropeptide Immunohistochemistry

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