A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

Takada, Silvia Honda; Sampaio, Carla; Allemandi, W.; Ito, P.H.; Takase, Luiz Fernando; Nogueira, Maria Inês

doi:10.1016/j.jneumeth.2011.03.009

Cited by 37 publications

(47 citation statements)

References 57 publications

(76 reference statements)

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“…A PE cannula (PE-50, Intramedic Clay Adams, Franklin Lakes, NJ) was next inserted into the carotid artery, allowing for the collection of a 3-ml blood sample. A blood gas and electrolyte automatic analyzer (Cobas b 121, Roche) was used for measurement of pH, PCO2, PO2, base excess, O2 saturation, HCO 3 Ϫ concentration, and hematocrit (36).…”

Section: ϫ5mentioning

confidence: 99%

Aortocaval fistula delays gastric emptying of liquid test meal in awake rats

Silva

Palheta

Oliveira

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Silva MT, Palheta RC Jr, Oliveira FG, de Lima JB, AntunesRodrigues J, Oliveira RB, Magalhães PJ, Santos AA. Aortocaval fistula delays gastric emptying of liquid test meal in awake rats. Am J Physiol Heart Circ Physiol 304: H1397-H1405, 2013. First published March 15, 2013 doi:10.1152/ajpheart.00827.2012.-Arteriovenous anastomoses disrupt cardiovascular and renal homeostasis, eliciting hemodynamic adjustments, resetting the humoral pattern, and inducing cardiac hypertrophy. Because acute circulatory imbalance alters gut motor behavior, we studied the effects of arteriovenous fistula placement on the gastric emptying (GE) of a liquid meal in awake rats. After laparotomy, we created an aortocaval fistula (ACF) by aorta and cava wall puncture with a 21-, 23-, or 26-gauge needle. The ACF was not created in the control group, which underwent sham operation. After 12, 24, or 48 h, mean arterial pressure, heart rate, and central venous pressure were continuously recorded, and cardiac output was estimated by thermal dilution. The rats were then gavage fed a test meal (i.e., phenol red in glucose solution), and fractional dye retention was determined 10, 20, or 30 min later. The effect of prior bleeding on ACF-induced GE delay, the role of neuroautonomic pathways, and changes in plasma hormone levels (i.e., angiotensin II, arginine vasopressin, atrial natriuretic peptide, corticosterone, and oxytocin) were evaluated. When compared with the sham-operated group, ACF rats exhibited arterial hypotension, higher (P Ͻ 0.05) heart rate, central venous pressure, and cardiac output values and increased (P Ͻ 0.05) gastric dye retention, a phenomenon prevented by bilateral subdiaphragmatic vagotomy and hexamethonium treatment. Pirenzepine also impaired the occurrence of gastric delay in subjects with ACF. In addition to causing hyperkinetic circulation, ACF placement delayed the GE of liquid in awake rats, an effect that likely involves a parasympathetic pathway. arteriovenous fistula; gastrointestinal motility; hyperkinetic circulation; intestinal transit; natriuretic hormone HYPERKINETIC CIRCULATION IS a systemic condition that may originate from an arteriovenous (AV) fistula, either as a congenital malformation or acquired after invasive procedures (e.g., percutaneous renal biopsy), surgeries (e.g., nephrectomy), or trauma (e.g., stab or gunshot wounds) (11,24). Large AV fistulas are considered a simple and reliable model to elicit congestive heart failure (13). Laboratory animals with an aortocaval fistula (ACF) present hemodynamic and neurohumoral changes that resemble those seen in patients with heart failure, including cardiac hypertrophy (22). These animals have high levels of cardiac output (CO) and mean circulatory filling pressure, in addition to tachycardia, low resistance to blood flow in circulatory vessels, and decreased mean arterial pressure (MAP) (17).As a consequence of inducing such changes, the placement of an AV fistula activates complex neurohumoral mechanisms that may restore hemodynamic homeostasis. During ...

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Section: ϫ5mentioning

confidence: 99%

Aortocaval fistula delays gastric emptying of liquid test meal in awake rats

Silva

Palheta

Oliveira

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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“…Aos animais que foram submetidos a procedimentos anóxicos (nitrogênio gasoso 100%), foi utilizada uma câmara semi-hermética de policarbonato com 31,0  14,0  19,5 cm com uma entrada e saída de gás, acoplada ao manômetro, fluxômetro e ao cilindro de nitrogênio gasoso (TAKADA et al, 2011).…”

Section: Condições Experimentaisunclassified

“…condições anóxicas é descrito como igual a 12,5% (TAKADA et al, 2011;IUVONE et al, 1996;DELL'ANNA et al, 1995;1991).…”

Section: Condições Experimentaisunclassified

“…O modelo experimental escolhido para provocar anóxia em neonatos, foi adaptado da literatura (ROGALSKA et al, 2006;TANG e NAKAZAWA, 2005;CAPUTA et al, 2005;LAVIOLA et al, 2004;DELL'ANNA et al, 1997;IUVONE et al, 1996;BUWALDA et al, 1995), e validado por nosso grupo de pesquisa (TAKADA et al, 2011). Entretanto, dentro de um mesmo grupo experimental a espessura foi constante.…”

Section: Condições Experimentaisunclassified

“…Nessa condição, são relatados ainda distúrbios neuro-comportamentais, tais como prejuízo no aprendizado e na memória espacial (DELL'ANNA et al, 1997;IUVONE et al, 1996), que persistem por toda vida (CAPUTA et al, 2005;BUWALDA et al, 1995). Nosso modelo validado indicou que essa situação é verdadeira para o NTS, quando o neonato é exposto a 25 minutos de privação de oxigênio (TAKADA et al, 2011).…”

Section: Irunclassified

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Estudo da imunorreatividade da proteína S100b no Hipocampo e Núcleo do Trato Solitário de ratos neonatos submetido à anóxia.

Allemandi¹

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Sensorimotor development of male and female rats subjected to neonatal anoxia

Kumar

Helou

Petrucelli

et al. 2022

Developmental Psychobiology

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Neonatal hypoxic‐ischemic encephalopathy (HIE) is one of the most important reasons for morbidity and mortality in term‐born infants. HIE impacts early somatic, neurological, and motor development including social. To illustrate the damages in the sensorimotor system, an adapted and validated model of neonatal anoxia is used. This study evaluated the sex differences in Wistar rats, neurological reflex, and motor development at the suckling period. Short‐ and long‐term impairments associated with sex differences were observed. In general, anoxic males were more affected in comparison to their control group and to anoxic females. Long‐lasting effects of the injury in adolescent rats predominately affected males. Similar to previous studies, we also found a decrease in the number of the substantia nigra cells in both sexes, compared to their control. So far, the results indicate that HIE caused neurobehavioral alterations and asymmetrical motor behavior with brain damage, possibly related to cognitive impairments previously observed at adolescence. These alterations may represent a useful endpoint for studying the efficacy of potential strategies that may improve the developmental consequences of a perinatal asphyxia insult in humans.

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A modified rat model of neonatal anoxia: Development and evaluation by pulseoximetry, arterial gasometry and Fos immunoreactivity

Cited by 37 publications

References 57 publications

Aortocaval fistula delays gastric emptying of liquid test meal in awake rats

Aortocaval fistula delays gastric emptying of liquid test meal in awake rats

Estudo da imunorreatividade da proteína S100b no Hipocampo e Núcleo do Trato Solitário de ratos neonatos submetido à anóxia.

Sensorimotor development of male and female rats subjected to neonatal anoxia

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