Expression of Flt3 and c-kit during growth and maturation of human CD34+CD38− cells

Flk-2 is a marker in hematopoietic stem cell differentiation: A simple method to isolate long-term stem cells

“…The Thy-1.1 Ϫ Flk-2 ϩ population yielded similar multilineage reconstitution at low (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) cell numbers (Table 2), although burst size was small. Because the burst size is minimal from the Thy-1.1 Ϫ Flk-2 ϩ population, recipient mice were killed at 2-3 weeks.…”

“…Flk-2 was cloned initially from a highly purified fetal liver HSC library and shows a high degree of homology to the c-kit and c-fms receptors (9,10). Flk-2 stimulated by the flt3 ligand has been implicated as both a survival (11) and a proliferative signal (12) of primitive hematopoietic progenitors, acting in synergy with the receptors for steel factor, IL-6, IL-11, granulocyte colony-stimulating factor, or thrombopoietin in enhancing the expansion of both murine (11,(13)(14)(15)(16) and human (17)(18)(19)(20) primitive progenitors.…”

Identification of Long-Term Repopulating Potential of Human Cord Blood-Derived CD34−flt3− Severe Combined Immunodeficiency-Repopulating Cells by Intra-Bone Marrow Injection

“…It is well documented that the tyrosine kinase receptors c-kit and flt3 are expressed and function in early mouse [1][2][3][4][5][6][7][8][9][10] and human hematopoiesis [1,[11][12][13][14][15][16][17][18][19][20][21]. Moreover, their respective ligands, stem cell factor (SCF) and flt3 ligand (FL), synergistically act with each other and play an important role in the regulation (generation, maintenance, proliferation, differentiation, and expansion) of early stages of murine and human candidate hematopoietic stem cells (HSCs) .…”

Flt3‐ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)‐4‐ and VLA‐5‐dependent mechanism

“…Flt3‐ligand (FL), the ligand for the tyrosine kinase receptor flt3, is a regulator of early haematopoiesis. The expression of flt3 is limited to the CD34 + cell compartment and is rapidly lost upon HPC differentiation (Xiao et al , 1999). Restricted expression of flt3 on immature HPC correlates with the action of FL on the early progenitor compartment (Lyman & Jacobsen, 1998).…”